![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
JL Cereghino, EG Marcusson and SD Emr
Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California, School of Medicine, San Diego, La Jolla 92093-0668, USA.
VPS10 of Saccharomyces cerevisiae encodes a type I transmembrane receptor protein required for the sorting of the soluble vacuolar hydrolase carboxypeptidase Y (CPY). To characterize the essential structural features and intercompartmental transport itinerary of the CPY receptor, we have constructed mutant forms of Vps10p that alter the carboxyterminal cytoplasmic tail of the protein. In addition, we have analyzed the effect these mutations as well as mutations in several VPS genes have on the function, stability, and localization of Vps10p. Although wild-type Vps10p is very stable over a 3-h chase period, overproduction of Vps10p results in PEP4-dependent degradation of the receptor. Immunofluorescence studies indicate that overexpressed receptor is delivered to the vacuole. A mutant form of Vps10p, in which 157 residues of the 164-residue cytoplasmic tail domain have been deleted, missorts CPY and is degraded rapidly. Additional mutations in the carboxy-terminus of Vps10p, including a deletion of a putative retention/recycling signal (FYVF), also result in CPY missorting and PEP4-dependent receptor instability. Because the cytoplasmic tail domain may interact with other factors, possibly VPS gene products, Vps10p stability was examined in a number of vps mutants. As was observed with the late Golgi protein Kex2p, Vps10p is unstable in a vps1 mutant. However, instability of Vps10p is even more severe in the class E vps mutants. Double mutant analyses demonstrate that this rapid degradation is dependent upon vacuolar proteases and a functional vacuolar ATPase. Fractionation studies of Vps10p in class E vps mutant strains indicate that the turnover of Vps10p occurs in a compartment other than the vacuole. These data are consistent with a model in which the cytoplasmic tail of Vps10p directs cycling of the receptor between a late Golgi sorting compartment and a prevacuolar endosome-like compartment, an exaggerated form of which is present in the vps class E mutants.
This article has been cited by other articles:
|
|
 |
|
  S. Codlin and S. E. Mole S. pombe btn1, the orthologue of the Batten disease gene CLN3, is required for vacuole protein sorting of Cpy1p and Golgi exit of Vps10p J. Cell Sci., April 15, 2009; 122(8): 1163 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Abazeed and R. S. Fuller Yeast Golgi-localized, {gamma}-Ear-containing, ADP-Ribosylation Factor-binding Proteins Are but Adaptor Protein-1 Is Not Required for Cell-free Transport of Membrane Proteins from the Trans-Golgi Network to the Prevacuolar Compartment Mol. Biol. Cell, November 1, 2008; 19(11): 4826 - 4836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Morishita and J. Engebrecht Sorting Signals within the Saccharomyces cerevisiae Sporulation-Specific Dityrosine Transporter, Dtr1p, C Terminus Promote Golgi-to-Prospore Membrane Transport Eukaryot. Cell, October 1, 2008; 7(10): 1674 - 1684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schluter, K. K.Y. Lam, J. Brumm, B. W. Wu, M. Saunders, T. H. Stevens, J. Bryan, and E. Conibear Global Analysis of Yeast Endosomal Transport Identifies the Vps55/68 Sorting Complex Mol. Biol. Cell, April 1, 2008; 19(4): 1282 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chidambaram, J. Zimmermann, and G. F. von Mollard ENTH domain proteins are cargo adaptors for multiple SNARE proteins at the TGN endosome J. Cell Sci., February 1, 2008; 121(3): 329 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. Strochlic, T. G. Setty, A. Sitaram, and C. G. Burd Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling J. Cell Biol., April 9, 2007; 177(1): 115 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Liu, Z. Hua, J. A. Nepute, and T. R. Graham Yeast P4-ATPases Drs2p and Dnf1p Are Essential Cargos of the NPFXD/Sla1p Endocytic Pathway Mol. Biol. Cell, February 1, 2007; 18(2): 487 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Curtiss, C. Jones, and M. Babst Efficient Cargo Sorting by ESCRT-I and the Subsequent Release of ESCRT-I from Multivesicular Bodies Requires the Subunit Mvb12 Mol. Biol. Cell, February 1, 2007; 18(2): 636 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Furuta, K. Fujimura-Kamada, K. Saito, T. Yamamoto, and K. Tanaka Endocytic Recycling in Yeast Is Regulated by Putative Phospholipid Translocases and the Ypt31p/32p-Rcy1p Pathway Mol. Biol. Cell, January 1, 2007; 18(1): 295 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L.P. daSilva, O. Foresti, and J. Denecke Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail PLANT CELL, June 1, 2006; 18(6): 1477 - 1497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Iwaki, A. Hosomi, S. Tokudomi, Y. Kusunoki, Y. Fujita, Y. Giga-Hama, N. Tanaka, and K. Takegawa Vacuolar protein sorting receptor in Schizosaccharomyces pombe. Microbiology, May 1, 2006; 152(Pt 5): 1523 - 1532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-W. Shin, H. Kobayashi, M. Kitamura, S. Waguri, T. Suganuma, Y. Uchiyama, and K. Nakayama Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking J. Cell Sci., September 1, 2005; 118(17): 4039 - 4048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dennes, C. Cromme, K. Suresh, N. S. Kumar, J. A. Eble, A. Hahnenkamp, and R. Pohlmann The Novel Drosophila Lysosomal Enzyme Receptor Protein Mediates Lysosomal Sorting in Mammalian Cells and Binds Mammalian and Drosophila GGA Adaptors J. Biol. Chem., April 1, 2005; 280(13): 12849 - 12857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Johnston, C. Foote, A. Santeford, and S. F. Nothwehr Golgi-to-Late Endosome Trafficking of the Yeast Pheromone Processing Enzyme Ste13p Is Regulated by a Phosphorylation Site in its Cytosolic Domain Mol. Biol. Cell, March 1, 2005; 16(3): 1456 - 1468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Abazeed, J. M. Blanchette, and R. S. Fuller Cell-free Transport from the trans-Golgi Network to Late Endosome Requires Factors Involved in Formation and Consumption of Clathrin-coated Vesicles J. Biol. Chem., February 11, 2005; 280(6): 4442 - 4450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Blanchette, M. E. Abazeed, and R. S. Fuller Cell-free Reconstitution of Transport from the trans-Golgi Network to the Late Endosome/Prevacuolar Compartment J. Biol. Chem., November 19, 2004; 279(47): 48767 - 48773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Luhtala and G. Odorizzi Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes J. Cell Biol., August 30, 2004; 166(5): 717 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Parrish, C. J. Stefan, and S. D. Emr Essential Role for the Myotubularin-related Phosphatase Ymr1p and the Synaptojanin-like Phosphatases Sjl2p and Sjl3p in Regulation of Phosphatidylinositol 3-Phosphate in Yeast Mol. Biol. Cell, August 1, 2004; 15(8): 3567 - 3579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sipos, J. H. Brickner, E.J. Brace, L. Chen, A. Rambourg, F. Kepes, and R. S. Fuller Soi3p/Rav1p Functions at the Early Endosome to Regulate Endocytic Trafficking to the Vacuole and Localization of Trans-Golgi Network Transmembrane Proteins Mol. Biol. Cell, July 1, 2004; 15(7): 3196 - 3209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Katzmann, S. Sarkar, T. Chu, A. Audhya, and S. D. Emr Multivesicular Body Sorting: Ubiquitin Ligase Rsp5 Is Required for the Modification and Sorting of Carboxypeptidase S Mol. Biol. Cell, February 1, 2004; 15(2): 468 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. L. Yeo, L. Xu, J. Ren, V. J. Boulton, M. D. Wagle, C. Liu, G. Ren, P. Wong, R. Zahn, P. Sasajala, et al. Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae J. Cell Sci., October 1, 2003; 116(19): 3957 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Odorizzi, D. J. Katzmann, M. Babst, A. Audhya, and S. D. Emr Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae J. Cell Sci., May 15, 2003; 116(10): 1893 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Bache, C. Raiborg, A. Mehlum, and H. Stenmark STAM and Hrs Are Subunits of a Multivalent Ubiquitin-binding Complex on Early Endosomes J. Biol. Chem., March 28, 2003; 278(14): 12513 - 12521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Burda, S. M. Padilla, S. Sarkar, and S. D. Emr Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase J. Cell Sci., October 15, 2002; 115(20): 3889 - 3900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-B. Li, S. W. Rogers, Y. C. Tse, S. W. Lo, S. S. M. Sun, G.-Y. Jauh, and L. Jiang BP-80 and Homologs are Concentrated on Post-Golgi, Probable Lytic Prevacuolar Compartments Plant Cell Physiol., July 15, 2002; 43(7): 726 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Belgareh-Touze, S. Avaro, Y. Rouille, B. Hoflack, and R. Haguenauer-Tsapis Yeast Vps55p, a Functional Homolog of Human Obesity Receptor Gene-related Protein, Is Involved in Late Endosome to Vacuole Trafficking Mol. Biol. Cell, May 1, 2002; 13(5): 1694 - 1708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dennes, P. Madsen, M. S. Nielsen, C. M. Petersen, and R. Pohlmann The Yeast Vps10p Cytoplasmic Tail Mediates Lysosomal Sorting in Mammalian Cells and Interacts with Human GGAs J. Biol. Chem., March 29, 2002; 277(14): 12288 - 12293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Brickner, J. M. Blanchette, G. Sipos, and R. S. Fuller The Tlg SNARE complex is required for TGN homotypic fusion J. Cell Biol., December 10, 2001; 155(6): 969 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kawasaki-Nishi, K. Bowers, T. Nishi, M. Forgac, and T. H. Stevens The Amino-terminal Domain of the Vacuolar Proton-translocating ATPase a Subunit Controls Targeting and in Vivo Dissociation, and the Carboxyl-terminal Domain Affects Coupling of Proton Transport and ATP Hydrolysis J. Biol. Chem., December 7, 2001; 276(50): 47411 - 47420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mullins and J. S. Bonifacino Structural Requirements for Function of Yeast GGAs in Vacuolar Protein Sorting, alpha -Factor Maturation, and Interactions with Clathrin Mol. Cell. Biol., December 1, 2001; 21(23): 7981 - 7994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hirst, M. R. Lindsay, and M. S. Robinson Golgi-localized, gamma-Ear-containing, ADP-Ribosylation Factor-binding Proteins: Roles of the Different Domains and Comparison with AP-1 and Clathrin Mol. Biol. Cell, November 1, 2001; 12(11): 3573 - 3588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-A. Ha, J. T. Bunch, H. Hama, D. B. DeWald, and S. F. Nothwehr A Novel Mechanism for Localizing Membrane Proteins to Yeast Trans-Golgi Network Requires Function of Synaptojanin-like Protein Mol. Biol. Cell, October 1, 2001; 12(10): 3175 - 3190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Reddy and M. N.J. Seaman Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval Mol. Biol. Cell, October 1, 2001; 12(10): 3242 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Costaguta, C. J. Stefan, E. S. Bensen, S. D. Emr, and G. S. Payne Yeast Gga Coat Proteins Function with Clathrin in Golgi to Endosome Transport Mol. Biol. Cell, June 1, 2001; 12(6): 1885 - 1896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Deloche, B. G. Yeung, G. S. Payne, and R. Schekman Vps10p Transport from the trans-Golgi Network to the Endosome Is Mediated by Clathrin-coated Vesicles Mol. Biol. Cell, February 1, 2001; 12(2): 475 - 485. [Abstract] [Full Text]
|
|
|
|
|
|
R Zahn, B. Stevenson, S Schroder-Kohne, B Zanolari, H Riezman, and A. Munn End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae J. Cell Sci., January 5, 2001; 114(10): 1935 - 1947. [Abstract] [PDF]
|
|
|
|
|
|
E. S. Bensen, B. G. Yeung, and G. S. Payne Ric1p and the Ypt6p GTPase Function in a Common Pathway Required for Localization of Trans-Golgi Network Membrane Proteins Mol. Biol. Cell, January 1, 2001; 12(1): 13 - 26. [Abstract] [Full Text]
|
|
|
|
|
|
K. Bowers, B. P. Levi, F. I. Patel, and T. H. Stevens The Sodium/Proton Exchanger Nhx1p Is Required for Endosomal Protein Trafficking in the Yeast Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2000; 11(12): 4277 - 4294. [Abstract] [Full Text]
|
|
|
|
|
|
S. F. Nothwehr, S.-A. Ha, and P. Bruinsma Sorting of Yeast Membrane Proteins into an Endosome-to-Golgi Pathway Involves Direct Interaction of Their Cytosolic Domains with Vps35p J. Cell Biol., October 16, 2000; 151(2): 297 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hirst, W. W.Y. Lui, N. A. Bright, N. Totty, M. N.J. Seaman, and M. S. Robinson A Family of Proteins with {gamma}-Adaptin and Vhs Domains That Facilitate Trafficking between the Trans-Golgi Network and the Vacuole/Lysosome J. Cell Biol., April 3, 2000; 149(1): 67 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Cao, S. W. Rogers, J. Butler, L. Beevers, and J. C. Rogers Structural Requirements for Ligand Binding by a Probable Plant Vacuolar Sorting Receptor PLANT CELL, April 1, 2000; 12(4): 493 - 506. [Abstract] [Full Text]
|
|
|
|
|
|
W.-j. Luo and A. Chang An Endosome-to-Plasma Membrane Pathway Involved in Trafficking of a Mutant Plasma Membrane ATPase in Yeast Mol. Biol. Cell, February 1, 2000; 11(2): 579 - 592. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Gerrard, N. J. Bryant, and T. H. Stevens VPS21 Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment Mol. Biol. Cell, February 1, 2000; 11(2): 613 - 626. [Abstract] [Full Text]
|
|
|
|
|
|
E. Conibear and T. H. Stevens Vps52p, Vps53p, and Vps54p Form a Novel Multisubunit Complex Required for Protein Sorting at the Yeast Late Golgi Mol. Biol. Cell, January 1, 2000; 11(1): 305 - 323. [Abstract] [Full Text]
|
|
|
|
|
|
R. G. Spelbrink and S. F. Nothwehr The Yeast GRD20 Gene Is Required for Protein Sorting in the trans-Golgi Network/Endosomal System and for Polarization of the Actin Cytoskeleton Mol. Biol. Cell, December 1, 1999; 10(12): 4263 - 4281. [Abstract] [Full Text]
|
|
|
|
|
|
S. F. Nothwehr, P. Bruinsma, and L. A. Strawn Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment Mol. Biol. Cell, April 1, 1999; 10(4): 875 - 890. [Abstract] [Full Text]
|
|
|
|
|
|
M. Tsukada, E. Will, and D. Gallwitz Structural and Functional Analysis of a Novel Coiled-Coil Protein Involved in Ypt6 GTPase-regulated Protein Transport in Yeast Mol. Biol. Cell, January 1, 1999; 10(1): 63 - 75. [Abstract] [Full Text]
|
|
|
|
|
|
L. Jiang and J. C. Rogers Integral Membrane Protein Sorting to Vacuoles in Plant Cells: Evidence for Two Pathways J. Cell Biol., November 30, 1998; 143(5): 1183 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Sanderfoot, S. U. Ahmed, D. Marty-Mazars, I. Rapoport, T. Kirchhausen, F. Marty, and N. V. Raikhel A putative vacuolar cargo receptor partially colocalizes with AtPEP12p on a prevacuolar compartment in Arabidopsis roots PNAS, August 18, 1998; 95(17): 9920 - 9925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N.J. Seaman, J. Michael McCaffery, and S. D. Emr A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast J. Cell Biol., August 10, 1998; 142(3): 665 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Bryant and T. H. Stevens Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 230 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Voos and T. H. Stevens Retrieval of Resident Late-Golgi Membrane Proteins from the Prevacuolar Compartment of Saccharomyces cerevisiae Is Dependent on the Function of Grd19p J. Cell Biol., February 9, 1998; 140(3): 577 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. von Mollard and T. H. Stevens A Human Homolog Can Functionally Replace the Yeast Vesicle-associated SNARE Vti1p in Two Vesicle Transport Pathways J. Biol. Chem., January 30, 1998; 273(5): 2624 - 2630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Rieder and S. D. Emr A Novel RING Finger Protein Complex Essential for a Late Step in Protein Transport to the Yeast Vacuole Mol. Biol. Cell, November 1, 1997; 8(11): 2307 - 2327. [Abstract] [Full Text]
|
|
|
|
|
|
D. S. Yuan, A. Dancis, and R. D. Klausner Restriction of Copper Export in Saccharomyces cerevisiae to a Late Golgi or Post-Golgi Compartment in the Secretory Pathway J. Biol. Chem., October 10, 1997; 272(41): 25787 - 25793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Brickner and R. S. Fuller SOI1 Encodes a Novel, Conserved Protein That Promotes TGN-Endosomal Cycling of Kex2p and Other Membrane Proteins by Modulating the Function of Two TGN Localization Signals J. Cell Biol., October 6, 1997; 139(1): 23 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-j. Luo and A. Chang Novel Genes Involved in Endosomal Traffic in Yeast Revealed by Suppression of a Targeting-defective Plasma Membrane ATPase Mutant J. Cell Biol., August 25, 1997; 138(4): 731 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Piper, N. J. Bryant, and T. H. Stevens The Membrane Protein Alkaline Phosphatase Is Delivered to the Vacuole by a Route That Is Distinct from the VPS-dependent Pathway J. Cell Biol., August 11, 1997; 138(3): 531 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. v. Mollard, S. F. Nothwehr, and T. H. Stevens The Yeast v-SNARE Vti1p Mediates Two Vesicle Transport Pathways through Interactions with the t-SNAREs Sed5p and Pep12p J. Cell Biol., June 30, 1997; 137(7): 1511 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N.J. Seaman, E. G. Marcusson, J. L. Cereghino, and S. D. Emr Endosome to Golgi Retrieval of the Vacuolar Protein Sorting Receptor, Vps10p, Requires the Function of the VPS29, VPS30, and VPS35 Gene Products J. Cell Biol., April 7, 1997; 137(1): 79 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Bryant and T. H. Stevens Two Separate Signals Act Independently to Localize a Yeast Late Golgi Membrane Protein through a Combination of Retrieval and Retention J. Cell Biol., January 27, 1997; 136(2): 287 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nothwehr and A. Hindes The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi J. Cell Sci., January 5, 1997; 110(9): 1063 - 1072. [Abstract] [PDF]
|
|
|
|
|
|
S. Milgram, S. Kho, G. Martin, R. Mains, and B. Eipper Localization of integral membrane peptidylglycine alpha-amidating monooxygenase in neuroendocrine cells J. Cell Sci., January 3, 1997; 110(6): 695 - 706. [Abstract] [PDF]
|
|
|
|
|
|
B. F. Horazdovsky, C. R. Cowles, P. Mustol, M. Holmes, and S. D. Emr A Novel RING Finger Protein, Vps8p, Functionally Interacts with the Small GTPase, Vps21p, to Facilitate Soluble Vacuolar Protein Localization J. Biol. Chem., December 27, 1996; 271(52): 33607 - 33615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Tsukada and D Gallwitz Isolation and characterization of SYS genes from yeast, multicopy suppressors of the functional loss of the transport GTPase Ypt6p J. Cell Sci., January 10, 1996; 109(10): 2471 - 2481. [Abstract] [PDF]
|
|
|
|
 |
|
 J.H. Stack, P.K. Herman, D.B. DeWald, E.G. Marcusson, J. Lin Cereghino, B.F. Horazdovsky, and S.D. Emr Novel Protein Kinase/Phosphatidylinositol 3-Kinase Complex Essential for Receptor-mediated Protein Sorting to the Vacuole in Yeast Cold Spring Harb Symp Quant Biol, January 1, 1995; 60(0): 157 - 170. [Abstract] [PDF]
|
|
|
|
|
|
E. Harsay and R. Schekman A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway J. Cell Biol., January 21, 2002; 156(2): 271 - 286. [Abstract] [Full Text] [PDF]
|
|
