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LG Cao and YL Wang
Cell Biology Group, Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, USA.
The interaction between the mitotic spindle and the cellular cortex is thought to play a critical role in stimulating cell cleavage. However, little is understood about the nature of such interactions, particularly in tissue culture cells. We have investigated the role of the spindle midzone in signaling cytokinesis by creating a barrier in cultured epithelial cells with a blunted needle, to block signals that may emanate from this region. When the barrier was created during metaphase or early anaphase, cleavage took place only on the sides of the cortex facing the mitotic spindle. Microtubules on the cleaving side showed organization typical of that in normal dividing cells. On the noncleaving side, most microtubules passed from one side of the equator into the other without any apparent organization, and actin filaments failed to organize in the equatorial region. When the barrier was created after the first minute of anaphase, cells showed successful cytokinesis, with normal organization of microtubules and actin filaments on both sides of the barrier. Our study suggests that transient signals from the midzone of early anaphase spindles are required for equatorial contraction in cultured cells and that such signaling may involve the organization of microtubules near the equator.
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|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
W. B. Raich, A. N. Moran, J. H. Rothman, and J. Hardin Cytokinesis and Midzone Microtubule Organization in Caenorhabditis elegans Require the Kinesin-like Protein ZEN-4 Mol. Biol. Cell, August 1, 1998; 9(8): 2037 - 2049. [Abstract] [Full Text]
|
|
|
|
|
|
S. P. Wheatley, C. B. O'Connell, and Y.-l. Wang Inhibition of Chromosomal Separation Provides Insights into Cleavage Furrow Stimulation in Cultured Epithelial Cells Mol. Biol. Cell, August 1, 1998; 9(8): 2173 - 2184. [Abstract] [Full Text]
|
|
|
|
|
|
R. R. Adams, A. A.M. Tavares, A. Salzberg, H. J. Bellen, and D. M. Glover pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis Genes & Dev., May 15, 1998; 12(10): 1483 - 1494. [Abstract] [Full Text]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
R Neujahr, R Albrecht, J Kohler, M Matzner, J. Schwartz, M Westphal, and G Gerisch Microtubule-mediated centrosome motility and the positioning of cleavage furrows in multinucleate myosin II-null cells J. Cell Sci., January 5, 1998; 111(9): 1227 - 1240. [Abstract] [PDF]
|
|
|
|
|
|
K. Oegema and T. J. Mitchison Rappaport rules: Cleavage furrow induction in animal cells PNAS, May 13, 1997; 94(10): 4817 - 4820. [Full Text] [PDF]
|
|
|
|
|
|
C. L. Rieder, A. Khodjakov, L. V. Paliulis, T. M. Fortier, R. W. Cole, and G. Sluder Mitosis in vertebrate somatic cells with two spindles: Implications for the metaphase/anaphase transition checkpoint and cleavage PNAS, May 13, 1997; 94(10): 5107 - 5112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Eckley, A. M. Ainsztein, A. M. Mackay, I. G. Goldberg, and W. C. Earnshaw Chromosomal Proteins and Cytokinesis: Patterns of Cleavage Furrow Formation and Inner Centromere Protein Positioning in Mitotic Heterokaryons and Mid-anaphase Cells J. Cell Biol., March 24, 1997; 136(6): 1169 - 1183. [Abstract] [Full Text] [PDF]
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G. Walker, C. Shuster, and D. Burgess Microtubule-entrained kinase activities associated with the cortical cytoskeleton during cytokinesis J. Cell Sci., January 6, 1997; 110(12): 1373 - 1386. [Abstract] [PDF]
|
|
|
|
|
|
H Ohkura, T Torok, G Tick, J Hoheisel, I Kiss, and D. Glover Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis J. Cell Sci., January 4, 1997; 110(8): 945 - 954. [Abstract] [PDF]
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A Paoletti, M Moudjou, M Paintrand, J. Salisbury, and M Bornens Most of centrin in animal cells is not centrosome-associated and centrosomal centrin is confined to the distal lumen of centrioles J. Cell Sci., January 12, 1996; 109(13): 3089 - 3102. [Abstract] [PDF]
|
|
|
|
|
|
D. Fishkind, J. Silverman, and Y. Wang Function of spindle microtubules in directing cortical movement and actin filament organization in dividing cultured cells J. Cell Sci., January 8, 1996; 109(8): 2041 - 2051. [Abstract] [PDF]
|
|
|
|
 |
|
 M.-C. Chen, Y. Zhou, and H. W. Detrich III Zebrafish mitotic kinesin-like protein 1 (Mklp1) functions in embryonic cytokinesis Physiol Genomics, February 11, 2002; 8(1): 51 - 66. [Abstract] [Full Text] [PDF]
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