![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
J Wesseling, SW van der Valk and J Hilkens
Department of Tumor Biology, The Netherlands Cancer Institute (Antoni van Leeuwenhoekhuis), Amsterdam.
Episialin (MUC1, PEM, EMA, CA15-3 antigen) is a sialylated, membrane- associated glycoprotein with an extended mucin-like ectodomain. This domain mainly consists of 30-90 homologous 20-amino acid repeats that are rich in O-glycosylation sites (serines and threonines). It is likely that this part forms a polyproline beta-turn helix. As a result, the ectodomain can protrude more than 200 nm above the cell surface, whereas most cell surface molecules do not exceed a length of 35 nm. Normally, episialin is present at the apical side of glandular epithelial cells. On carcinoma cells, however, it can be strongly overexpressed and it is often present over the entire cell surface. We have previously shown that episialin, if it is interspersed between adhesion molecules, nonspecifically reduces cell-cell and cell- extracellular matrix interactions in vitro and in vivo, presumably by steric hindrance caused by the extreme length and high density of the episialin molecules at the cell surface. To analyze the molecular mechanism for this anti-adhesion effect in more detail, we have now deleted an increasing number of repeats in the episialin cDNA and transfected the resulting mutants into murine L929 cells expressing the homophilic adhesion molecule E-cadherin. Here we show that the length of episialin is the dominant factor that determines the inhibition of E- cadherin-mediated cell-cell interactions. For the anti-adhesive effect mediated by the full length episialin, charge repulsion by negatively charged sialylated O-linked glycans is far less important.
This article has been cited by other articles:
|
|
 |
|
  N. Dharmaraj, S. J. Gendler, and D. D. Carson Expression of Human MUC1 During Early Pregnancy in the Human MUC1 Transgenic Mouse Model Biol Reprod, December 1, 2009; 81(6): 1182 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhao, X. Guo, G. B. Nash, P. C. Stone, J. Hilkens, J. M. Rhodes, and L.-G. Yu Circulating Galectin-3 Promotes Metastasis by Modifying MUC1 Localization on Cancer Cell Surface Cancer Res., September 1, 2009; 69(17): 6799 - 6806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aubert, V. Fauquette, B. Hemon, R. Lepoivre, N. Briez, D. Bernard, I. Van Seuningen, X. Leroy, and M. Perrais MUC1, a New Hypoxia Inducible Factor Target Gene, Is an Actor in Clear Renal Cell Carcinoma Tumor Progression Cancer Res., July 15, 2009; 69(14): 5707 - 5715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Patriarca, P. Colombo, A. Pio Taronna, J. Wesseling, G. Franchi, F. Guddo, R. Naspro, R. M. Macchi, P. Giunta, M. Di Pasquale, et al. Cell Discohesion and Multifocality of Carcinoma In situ of the Bladder: New Insight From the Adhesion Molecule Profile (e-Cadherin, Ep-CAM, and MUC1) International Journal of Surgical Pathology, April 1, 2009; 17(2): 99 - 106. [Abstract] [PDF]
|
|
|
|
 |
|
 U. Telang, D. A. Brazeau, and M. E. Morris Comparison of the Effects of Phenethyl Isothiocyanate and Sulforaphane on Gene Expression in Breast Cancer and Normal Mammary Epithelial Cells Experimental Biology and Medicine, March 1, 2009; 234(3): 287 - 295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Chauhan, K. Vannatta, M. C. Ebeling, N. Vinayek, A. Watanabe, K. K. Pandey, M. C. Bell, M. D. Koch, H. Aburatani, Y. Lio, et al. Expression and Functions of Transmembrane Mucin MUC13 in Ovarian Cancer Cancer Res., February 1, 2009; 69(3): 765 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Katz, K. Skorecki, and M. Tzukerman Niche-Dependent Tumorigenic Capacity of Malignant Ovarian Ascites-Derived Cancer Cell Subpopulations Clin. Cancer Res., January 1, 2009; 15(1): 70 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wang, J. A Julian, and D. D Carson The MUC1 HMFG1 Glycoform Is a Precursor to the 214D4 Glycoform in the Human Uterine Epithelial Cell Line, HES Biol Reprod, February 1, 2008; 78(2): 290 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sumiyoshi, J. Ricciuto, A. Tisdale, I. K. Gipson, F. Mantelli, and P. Argueso Antiadhesive Character of Mucin O-glycans at the Apical Surface of Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 197 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Swanson, K. M. McDermott, P. K. Singh, J. P. Eggers, P. R. Crocker, and M. A. Hollingsworth MUC1 Is a Counter-Receptor for Myelin-Associated Glycoprotein (Siglec-4a) and Their Interaction Contributes to Adhesion in Pancreatic Cancer Perineural Invasion Cancer Res., November 1, 2007; 67(21): 10222 - 10229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Yang, K. Q. Cai, I. H. Roland, E. R. Smith, and X.-X. Xu Disabled-2 Is an Epithelial Surface Positioning Gene J. Biol. Chem., April 27, 2007; 282(17): 13114 - 13122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-G. Yu, N. Andrews, Q. Zhao, D. McKean, J. F. Williams, L. J. Connor, O. V. Gerasimenko, J. Hilkens, J. Hirabayashi, K. Kasai, et al. Galectin-3 Interaction with Thomsen-Friedenreich Disaccharide on Cancer-associated MUC1 Causes Increased Cancer Cell Endothelial Adhesion J. Biol. Chem., January 5, 2007; 282(1): 773 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Longman, R. Poulsom, A. P. Corfield, B. F. Warren, N. A. Wright, and M. G. Thomas Alterations in the Composition of the Supramucosal Defense Barrier in Relation to Disease Severity of Ulcerative Colitis J. Histochem. Cytochem., December 1, 2006; 54(12): 1335 - 1348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Achache and A. Revel Endometrial receptivity markers, the journey to successful embryo implantation Hum. Reprod. Update, November 1, 2006; 12(6): 731 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Parry, F. G. Hanisch, S.-H. Leir, M. Sutton-Smith, H. R. Morris, A. Dell, and A. Harris N-Glycosylation of the MUC1 mucin in epithelial cells and secretions Glycobiology, July 1, 2006; 16(7): 623 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Kinlough, R. J. McMahan, P. A. Poland, J. B. Bruns, K. L. Harkleroad, R. J. Stremple, O. B. Kashlan, K. M. Weixel, O. A. Weisz, and R. P. Hughey Recycling of MUC1 Is Dependent on Its Palmitoylation J. Biol. Chem., April 28, 2006; 281(17): 12112 - 12122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Julien, E. Adriaenssens, K. Ottenberg, A. Furlan, G. Courtand, A.-S. Vercoutter-Edouart, F.-G. Hanisch, P. Delannoy, and X. Le Bourhis ST6GalNAc I expression in MDA-MB-231 breast cancer cells greatly modifies their O-glycosylation pattern and enhances their tumourigenicity Glycobiology, January 1, 2006; 16(1): 54 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Agrawal and B. M. Longenecker MUC1 mucin-mediated regulation of human T cells Int. Immunol., April 1, 2005; 17(4): 391 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Kinlough, P. A. Poland, J. B. Bruns, K. L. Harkleroad, and R. P. Hughey MUC1 Membrane Trafficking Is Modulated by Multiple Interactions J. Biol. Chem., December 17, 2004; 279(51): 53071 - 53077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Herbert, J. F. Grosso, M. Dorsey Jr., T. Fu, I. Keydar, M. A. Cejas, D. H. Wreschner, N. Smorodinski, and D. M. Lopez A Unique Mucin Immunoenhancing Peptide with Antitumor Properties Cancer Res., November 1, 2004; 64(21): 8077 - 8084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Hamada, S Matsukita, M Goto, S Kitajima, S K Batra, T Irimura, K Sueyoshi, K Sugihara, and S Yonezawa Mucin expression in pleomorphic adenoma of salivary gland: a potential role for MUC1 as a marker to predict recurrence J. Clin. Pathol., August 1, 2004; 57(8): 813 - 821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hayashi, W. W.-Y. Kao, N. Kohno, M. Nishihara-Hayashi, A. Shiraishi, T. Uno, M. Yamaguchi, S. Okamoto, M. Maeda, T. Ikeda, et al. Expression Patterns of Sialylated Epitope Recognized by KL-6 Monoclonal Antibody in Ocular Surface Epithelium of Normals and Dry Eye Patients Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2212 - 2217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lapointe, C. Li, J. P. Higgins, M. van de Rijn, E. Bair, K. Montgomery, M. Ferrari, L. Egevad, W. Rayford, U. Bergerheim, et al. Gene expression profiling identifies clinically relevant subtypes of prostate cancer PNAS, January 20, 2004; 101(3): 811 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Singh, N. Moniaux, S. C. Chauhan, J. L. Meza, and S. K. Batra Inhibition of MUC4 Expression Suppresses Pancreatic Tumor Cell Growth and Metastasis Cancer Res., January 15, 2004; 64(2): 622 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tsuiji, S. Takasaki, M. Sakamoto, T. Irimura, and S. Hirohashi Aberrant O-glycosylation inhibits stable expression of dysadherin, a carcinoma-associated antigen, and facilitates cell-cell adhesion Glycobiology, July 1, 2003; 13(7): 521 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Thathiah, C. P. Blobel, and D. D. Carson Tumor Necrosis Factor-alpha Converting Enzyme/ADAM 17 Mediates MUC1 Shedding J. Biol. Chem., January 24, 2003; 278(5): 3386 - 3394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bailly, Z. Zhang, W. Meier, R. Cate, M. Sanicola, and J. V. Bonventre Shedding of Kidney Injury Molecule-1, a Putative Adhesion Protein Involved in Renal Regeneration J. Biol. Chem., October 11, 2002; 277(42): 39739 - 39748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wykes, K. P. A. MacDonald, M. Tran, R. J. Quin, P. X. Xing, S. J. Gendler, D. N. J. Hart, and M. A. McGuckin MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells J. Leukoc. Biol., October 1, 2002; 72(4): 692 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Evangelou, M. Letarte, A. Marks, and T. J. Brown Androgen Modulation of Adhesion and Antiadhesion Molecules in PC-3 Prostate Cancer Cells Expressing Androgen Receptor Endocrinology, October 1, 2002; 143(10): 3897 - 3904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Samulowitz, A. Kuhn, G. Brachtendorf, R. Nawroth, A. Braun, A. Bankfalvi, W. Bocker, and D. Vestweber Human Endomucin : Distribution Pattern, Expression on High Endothelial Venules, and Decoration with the MECA-79 Epitope Am. J. Pathol., May 1, 2002; 160(5): 1669 - 1681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Van Aken, P. Papeleu, Patrick De Potter, E. Bruyneel, J. Philippe, S. Seregard, A. Kvanta, J.-J. De Laey, and M. M. Mareel Structure and Function of the N-Cadherin/Catenin Complex in Retinoblastoma Invest. Ophthalmol. Vis. Sci., March 1, 2002; 43(3): 595 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R L Ten Berge, F G M Snijdewint, S von Mensdorff-Pouilly, R J J Poort-Keesom, J J Oudejans, J W R Meijer, R Willemze, J Hilgers, and C J L M Meijer MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form J. Clin. Pathol., December 1, 2001; 54(12): 933 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Andrianifahanana, N. Moniaux, B. M. Schmied, J. Ringel, H. Friess, M. A. Hollingsworth, M. W. Buchler, J.-P. Aubert, and S. K. Batra Mucin (MUC) Gene Expression in Human Pancreatic Adenocarcinoma and Chronic Pancreatitis: A Potential Role of MUC4 as a Tumor Marker of Diagnostic Significance Clin. Cancer Res., December 1, 2001; 7(12): 4033 - 4040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Giatromanolaki, M. I. Koukourakis, E. Sivridis, J. Pastorek, C. C. Wykoff, K. C. Gatter, and A. L. Harris Expression of Hypoxia-inducible Carbonic Anhydrase-9 Relates to Angiogenic Pathways and Independently to Poor Outcome in Non-Small Cell Lung Cancer Cancer Res., November 1, 2001; 61(21): 7992 - 7998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Papadopoulos, E. Sivridis, A. Giatromanolaki, and M. I. Koukourakis Tumor Angiogenesis Is Associated with MUC1 Overexpression and Loss of Prostate-specific Antigen Expression in Prostate Cancer Clin. Cancer Res., June 1, 2001; 7(6): 1533 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Guruswamy, S. Lightfoot, M. A. Gold, R. Hassan, K. D. Berlin, R. T. Ivey, and D. M. Benbrook Effects of Retinoids on Cancerous Phenotype and Apoptosis in Organotypic Cultures of Ovarian Carcinoma J Natl Cancer Inst, April 4, 2001; 93(7): 516 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bennett , Jr., T. Järvelä, P. Engelhardt, L. Kostamovaara, P. Sparks, O. Carpén, O. Turunen, and A. Vaheri Mucin MUC1 Is Seen in Cell Surface Protrusions Together with Ezrin in Immunoelectron Tomography and is Concentrated at Tips of Filopodial Protrusions in MCF-7 Breast Carcinoma Cells J. Histochem. Cytochem., January 1, 2001; 49(1): 67 - 78. [Abstract] [Full Text]
|
|
|
|
 |
|
 G.D. Offner and R.F. Troxler Heterogeneity of High-molecular-weight Human Salivary Mucins Advances in Dental Research, December 1, 2000; 14(1): 69 - 75. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Takeda, W. Y. Go, R. A. Orlando, and M. G. Farquhar Expression of Podocalyxin Inhibits Cell-Cell Adhesion and Modifies Junctional Properties in Madin-Darby Canine Kidney Cells Mol. Biol. Cell, September 1, 2000; 11(9): 3219 - 3232. [Abstract] [Full Text]
|
|
|
|
|
|
A. Giatromanolaki, M. I. Koukourakis, E. Sivridis, K. O'Byrne, G. Cox, P. E. Thorpe, K. C. Gatter, and A. L. Harris Coexpression of MUC1 Glycoprotein with Multiple Angiogenic Factors in Non-Small Cell Lung Cancer Suggests Coactivation of Angiogenic and Migration Pathways Clin. Cancer Res., May 1, 2000; 6(5): 1917 - 1921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Chang and E. Szabo Induction of Differentiation and Apoptosis by Ligands of Peroxisome Proliferator-activated Receptor {{gamma}} in Non-Small Cell Lung Cancer Cancer Res., February 1, 2000; 60(4): 1129 - 1138. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Rounds, L. L. Likar, E. O. Harrington, K. C. Kim, A. Smeglin, K. Heins, and N. Parks Nucleotide-induced PMN adhesion to cultured epithelial cells: possible role of MUC1 mucin Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L874 - L880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aono, S. Nakagawa, A. B. Reynolds, and M. Takeichi p120ctn Acts as an Inhibitory Regulator of Cadherin Function in Colon Carcinoma Cells J. Cell Biol., May 3, 1999; 145(3): 551 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ohgami, T. Tsuda, T. Osaki, T. Mitsudomi, Y. Morimoto, T. Higashi, and K. Yasumoto MUC1 mucin mRNA expression in stage I lung adenocarcinoma and its association with early recurrence Ann. Thorac. Surg., March 1, 1999; 67(3): 810 - 814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. CHANEZ, A. M. VIGNOLA, P. VIC, F. GUDDO, G. BONSIGNORE, P. GODARD, and J. BOUSQUET Comparison between Nasal and Bronchial Inflammation in Asthmatic and Control Subjects Am. J. Respir. Crit. Care Med., February 1, 1999; 159(2): 588 - 595. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Scholl, C Gamallo, S Vilar?o, and M Quintanilla Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes J. Cell Sci., January 12, 1999; 112(24): 4601 - 4613. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Li, A. Bharti, D. Chen, J. Gong, and D. Kufe Interaction of Glycogen Synthase Kinase 3beta with the DF3/MUC1 Carcinoma-Associated Antigen and beta -Catenin Mol. Cell. Biol., December 1, 1998; 18(12): 7216 - 7224. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. M. Tempero, M. L. VanLith, K. Morikane, G. J. Rowse, S. J. Gendler, and M. A. Hollingsworth CD4+ Lymphocytes Provide MUC1-Specific Tumor Immunity In Vivo That Is Undetectable In Vitro and Is Absent in MUC1 Transgenic Mice J. Immunol., November 15, 1998; 161(10): 5500 - 5506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Komatsu, C. A. C. Carraway, N. L. Fregien, and K. L. Carraway Reversible Disruption of Cell-Matrix and Cell-Cell Interactions by Overexpression of Sialomucin Complex J. Biol. Chem., December 26, 1997; 272(52): 33245 - 33254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Litvinov, M. Balzar, M. J. Winter, H. A.M. Bakker, I. H. B.-d. Bruijn, F. Prins, G. J. Fleuren, and S. O. Warnaar Epithelial Cell Adhesion Molecule (Ep-CAM) Modulates Cell-Cell Interactions Mediated by Classic Cadherins J. Cell Biol., December 1, 1997; 139(5): 1337 - 1348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Arcasoy, J. Latoche, M. Gondor, S. C. Watkins, R. A. Henderson, R. Hughey, O. J. Finn, and J. M. Pilewski MUC1 and Other Sialoglycoconjugates Inhibit Adenovirus-mediated Gene Transfer to Epithelial Cells Am. J. Respir. Cell Mol. Biol., October 1, 1997; 17(4): 422 - 435. [Abstract] [Full Text]
|
|
|
|
|
|
M. Yamamoto, A. Bharti, Y. Li, and D. Kufe Interaction of the DF3/MUC1 Breast Carcinoma-associated Antigen and beta -Catenin in Cell Adhesion J. Biol. Chem., May 9, 1997; 272(19): 12492 - 12494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Van Itallie and J. Anderson Occludin confers adhesiveness when expressed in fibroblasts J. Cell Sci., January 5, 1997; 110(9): 1113 - 1121. [Abstract] [PDF]
|
|
|
|
|
|
M. Goldberg, C. Peshkovsky, A. Shifteh, and Q. Al-Awqati {micro}-Protocadherin, a Novel Developmentally Regulated Protocadherin with Mucin-like Domains J. Biol. Chem., August 4, 2000; 275(32): 24622 - 24629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Gaemers, H. L. Vos, H. H. Volders, S. W. van der Valk, and J. Hilkens A STAT-responsive Element in the Promoter of the Episialin/MUC1 Gene Is Involved in Its Overexpression in Carcinoma Cells J. Biol. Chem., February 23, 2001; 276(9): 6191 - 6199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Schroeder, M. C. Thompson, M. M. Gardner, and S. J. Gendler Transgenic MUC1 Interacts with Epidermal Growth Factor Receptor and Correlates with Mitogen-activated Protein Kinase Activation in the Mouse Mammary Gland J. Biol. Chem., April 13, 2001; 276(16): 13057 - 13064. [Abstract] [Full Text] [PDF]
|
|
