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SE Rieder, LM Banta, K Kohrer, JM McCaffery and SD Emr
Department of Biology, Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, La Jolla 92093-0668, USA.
In the yeast Saccharomyces cerevisiae, vacuolar proteins such as carboxypeptidase Y transit from the Golgi to the lysosome-like vacuole via an endosome-like intermediate compartment. The vacuolar protein sorting (vps) mutant vps28, a member of the "class E" vps mutants, accumulates vacuolar, endocytic, and late Golgi markers in an aberrant endosome-like class E compartment. Sequence analysis of VPS28 revealed an open reading frame predicted to encode a hydrophilic protein of 242 amino acids. Consistent with this, polyclonal antiserum raised against Vps28p recognized a cytoplasmic protein of 28 kDa. Disruption of VPS28 resulted in moderate defects in both biosynthetic traffic and endocytic traffic destined for the vacuole. The transport of soluble vacuolar hydrolases to the vacuole was impaired in vps28 null mutant cells (approximately 40-50% carboxypeptidase Y missorted). Internalization of the endocytic marker FM 4-64, a vital lipophilic dye, resulted in intense staining of a small intracellular compartment adjacent to an enlarged vacuole in delta vps28 cells. Furthermore, the vacuolar H+- ATPase accumulated in the perivacuolar class E compartment in delta vps28 cells, as did a-factor receptor Ste3p that was internalized from the plasma membrane. Electron microscopic analysis revealed the presence of a novel compartment consisting of stacks of curved membrane cisternae. Immunolocalization studies demonstrated that the vacuolar H+- ATPase is associated with this cupped cisternal structure, indicating that it corresponds to the class E compartment observed by fluorescence microscopy. Our data indicate that kinetic defects in both anterograde and retrograde transport out of the prevacuolar compartment in vps28 mutants result in the accumulation of protein and membrane in an exaggerated multilamellar endosomal compartment. We propose that Vps28p, as well as other class E Vps proteins, may facilitate (possibly as coat proteins) the formation of transport intermediates required for efficient transport out of the prevacuolar endosome.
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A. Wiederkehr, S. Avaro, C. Prescianotto-Baschong, R. Haguenauer-Tsapis, and H. Riezman The F-Box Protein Rcy1p Is Involved in Endocytic Membrane Traffic and Recycling Out of an Early Endosome in Saccharomyces cerevisiae J. Cell Biol., April 17, 2000; 149(2): 397 - 410. [Abstract] [Full Text] [PDF]
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S. R. Gerrard, N. J. Bryant, and T. H. Stevens VPS21 Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment Mol. Biol. Cell, February 1, 2000; 11(2): 613 - 626. [Abstract] [Full Text]
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M. J. Lewis, B. J. Nichols, C. Prescianotto-Baschong, H. Riezman, and H. R. B. Pelham Specific Retrieval of the Exocytic SNARE Snc1p from Early Yeast Endosomes Mol. Biol. Cell, January 1, 2000; 11(1): 23 - 38. [Abstract] [Full Text]
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N. Bishop and P. Woodman ATPase-defective Mammalian VPS4 Localizes to Aberrant Endosomes and Impairs Cholesterol Trafficking Mol. Biol. Cell, January 1, 2000; 11(1): 227 - 239. [Abstract] [Full Text]
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C.-Y. Chen, M. F. Ingram, P. H. Rosal, and T. R. Graham Role for Drs2p, a P-Type Atpase and Potential Aminophospholipid Translocase, in Yeast Late Golgi Function J. Cell Biol., December 13, 1999; 147(6): 1223 - 1236. [Abstract] [Full Text] [PDF]
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R. G. Spelbrink and S. F. Nothwehr The Yeast GRD20 Gene Is Required for Protein Sorting in the trans-Golgi Network/Endosomal System and for Polarization of the Actin Cytoskeleton Mol. Biol. Cell, December 1, 1999; 10(12): 4263 - 4281. [Abstract] [Full Text]
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Y. Li, T. Kane, C. Tipper, P. Spatrick, and D. D. Jenness Yeast Mutants Affecting Possible Quality Control of Plasma Membrane Proteins Mol. Cell. Biol., May 1, 1999; 19(5): 3588 - 3599. [Abstract] [Full Text] [PDF]
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O. W. Rossanese, J. Soderholm, B. J. Bevis, I. B. Sears, J. O'Connor, E. K. Williamson, and B. S. Glick Golgi Structure Correlates with Transitional Endoplasmic Reticulum Organization in Pichia pastoris and Saccharomyces cerevisiae J. Cell Biol., April 5, 1999; 145(1): 69 - 81. [Abstract] [Full Text] [PDF]
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E. C. Gaynor, G. Mondésert, S. J. Grimme, S. I. Reed, P. Orlean, and S. D. Emr MCD4 Encodes a Conserved Endoplasmic Reticulum Membrane Protein Essential for Glycosylphosphatidylinositol Anchor Synthesis in Yeast Mol. Biol. Cell, March 1, 1999; 10(3): 627 - 648. [Abstract] [Full Text]
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J. Mulholland, J. Konopka, B. Singer-Kruger, M. Zerial, and D. Botstein Visualization of Receptor-mediated Endocytosis in Yeast Mol. Biol. Cell, March 1, 1999; 10(3): 799 - 817. [Abstract] [Full Text]
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C. J. Stefan and K. J. Blumer A Syntaxin Homolog Encoded by VAM3 Mediates Down-regulation of a Yeast G Protein-coupled Receptor J. Biol. Chem., January 15, 1999; 274(3): 1835 - 1841. [Abstract] [Full Text] [PDF]
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T. B. Reynolds, B. Diane Hopkins, M. R. Lyons, and T. R. Graham The High Osmolarity Glycerol Response (HOG) MAP Kinase Pathway Controls Localization of a Yeast Golgi Glycosyltransferase J. Cell Biol., November 16, 1998; 143(4): 935 - 946. [Abstract] [Full Text] [PDF]
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J. D. Gary, A. E. Wurmser, C. J. Bonangelino, L. S. Weisman, and S. D. Emr Fab1p Is Essential for PtdIns(3)P 5-Kinase Activity and the Maintenance of Vacuolar Size and Membrane Homeostasis J. Cell Biol., October 5, 1998; 143(1): 65 - 79. [Abstract] [Full Text] [PDF]
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T. K. Sato, T. Darsow, and S. D. Emr Vam7p, a SNAP-25-Like Molecule, and Vam3p, a Syntaxin Homolog, Function Together in Yeast Vacuolar Protein Trafficking Mol. Cell. Biol., September 1, 1998; 18(9): 5308 - 5319. [Abstract] [Full Text]
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T. Darsow, C. G. Burd, and S. D. Emr Acidic Di-leucine Motif Essential for AP-3-dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE J. Cell Biol., August 24, 1998; 142(4): 913 - 922. [Abstract] [Full Text] [PDF]
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R. Nass and R. Rao Novel Localization of a Na+/H+ Exchanger in a Late Endosomal Compartment of Yeast. IMPLICATIONS FOR VACUOLE BIOGENESIS J. Biol. Chem., August 14, 1998; 273(33): 21054 - 21060. [Abstract] [Full Text] [PDF]
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M. N.J. Seaman, J. Michael McCaffery, and S. D. Emr A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast J. Cell Biol., August 10, 1998; 142(3): 665 - 681. [Abstract] [Full Text] [PDF]
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B. Gerhardt, T. J. Kordas, C. M. Thompson, P. Patel, and T. Vida The Vesicle Transport Protein Vps33p Is an ATP-binding Protein That Localizes to the Cytosol in an Energy-dependent Manner J. Biol. Chem., June 19, 1998; 273(25): 15818 - 15829. [Abstract] [Full Text] [PDF]
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B. Wendland and S. D. Emr Pan1p, Yeast eps15, Functions as a Multivalent Adaptor That Coordinates Protein-Protein Interactions Essential for Endocytosis J. Cell Biol., April 6, 1998; 141(1): 71 - 84. [Abstract] [Full Text] [PDF]
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 N. J. Bryant and T. H. Stevens Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 230 - 247. [Abstract] [Full Text] [PDF]
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E. C. Gaynor, C.-Y. Chen, S. D. Emr, and T. R. Graham ARF Is Required for Maintenance of Yeast Golgi and Endosome Structure and Function Mol. Biol. Cell, March 1, 1998; 9(3): 653 - 670. [Abstract] [Full Text]
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B Singer-Kruger, Y Nemoto, L Daniell, S Ferro-Novick, and P De Camilli Synaptojanin family members are implicated in endocytic membrane traffic in yeast J. Cell Sci., January 11, 1998; 111(22): 3347 - 3356. [Abstract] [PDF]
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Genetic Interaction With vps8-200 Allows Partial Suppression of the Vestigial Vacuole Phenotype Caused by a pep5 Mutation in Saccharomyces cerevisiae Genetics, January 1, 1998; 148(1): 71 - 84.
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J. D. Stepp, K. Huang, and S. K. Lemmon The Yeast Adaptor Protein Complex, AP-3, Is Essential for the Efficient Delivery of Alkaline Phosphatase by the Alternate Pathway to the Vacuole J. Cell Biol., December 29, 1997; 139(7): 1761 - 1774. [Abstract] [Full Text] [PDF]
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S. E. Rieder and S. D. Emr A Novel RING Finger Protein Complex Essential for a Late Step in Protein Transport to the Yeast Vacuole Mol. Biol. Cell, November 1, 1997; 8(11): 2307 - 2327. [Abstract] [Full Text]
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