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Vol. 8, Issue 10, 1829-1844, October 1997
Department of Biology, Yale University, New Haven, Connecticut
06520-8103
Rom2p is a GDP/GTP exchange factor for Rho1p and Rho2p GTPases; Rho
proteins have been implicated in control of actin cytoskeletal rearrangements. ROM2 and RHO2 were
identified in a screen for high-copy number suppressors of
cik1
, a mutant defective in microtubule-based processes in Saccharomyces cerevisiae. A
Rom2p::3XHA fusion protein localizes to sites of polarized
cell growth, including incipient bud sites, tips of small buds, and
tips of mating projections. Disruption of ROM2 results
in temperature-sensitive growth defects at 11°C and 37°C.
rom2 cells exhibit morphological defects. At permissive temperatures, rom2 cells often form
elongated buds and fail to form normal mating projections after
exposure to pheromone; at the restrictive temperature, small budded
cells accumulate. High-copy number plasmids containing either
ROM2 or RHO2 suppress the
temperature-sensitive growth defects of cik1 and
kar3 strains. KAR3 encodes a
kinesin-related protein that interacts with Cik1p. Furthermore,
rom2 strains exhibit increased sensitivity to the microtubule depolymerizing drug benomyl. These results suggest a role
for Rom2p in both polarized morphogenesis and functions of the
microtubule cytoskeleton.
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