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Vol. 8, Issue 10, 2089-2099, October 1997
and
*Department of Biology, Fluorescently labeled myosin moved and accumulated
circumferentially in the equatorial region of dividing
Dictyostelium cells within a time course of 4 min,
followed by contraction of the contractile ring. To investigate the
mechanism of this transport process, we have expressed three mutant
myosins that cannot hydrolyze ATP in myosin null cells.
Immunofluorescence staining showed that these mutant myosins were also
correctly transported to the equatorial region, although no contraction
followed. The rates of transport, measured using green fluorescent
protein-fused myosins, were indistinguishable between wild-type and
mutant myosins. These observations demonstrate that myosin is passively
transported toward the equatorial region and incorporated into the
forming contractile ring without its own motor activity.
Bionic Design Group,
Molecular Biology of the Cell
Vol. 8, 2089-2099, October 1997
Copyright © 1997 by The American Society for Cell Biology
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