Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rolls, M. M.
Right arrow Articles by Machamer, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rolls, M. M.
Right arrow Articles by Machamer, C. E.

Vol. 8, Issue 11, 2111-2118, November 1997

Cholesterol-independent Targeting of Golgi Membrane Proteins in Insect Cells

Melissa M. Rolls,*dagger Marianne T. Marquardt,Dagger § Margaret Kielian,Dagger and Carolyn E. Machamer*par

 *Department of Cell Biology and Anatomy, Johns Hopkins Medical School, Baltimore, Maryland 21205; and  Dagger Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461

Distinct lipid compositions of intracellular organelles could provide a physical basis for targeting of membrane proteins, particularly where transmembrane domains have been shown to play a role. We tested the possibility that cholesterol is required for targeting of membrane proteins to the Golgi complex. We used insect cells for our studies because they are cholesterol auxotrophs and can be depleted of cholesterol by growth in delipidated serum. We found that two well-characterized mammalian Golgi proteins were targeted to the Golgi region of Aedes albopictus cells, both in the presence and absence of cellular cholesterol. Our results imply that a cholesterol gradient through the secretory pathway is not required for membrane protein targeting to the Golgi complex, at least in insect cells.

Molecular Biology of the Cell
Vol. 8, 2111-2118, November 1997
Copyright © 1997 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Physiol.Home page
O. Zamir and M. P. Charlton
Cholesterol and synaptic transmitter release at crayfish neuromuscular junctions
J. Physiol., February 15, 2006; 571(1): 83 - 99.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. M. Rolls, D. H. Hall, M. Victor, E. H. K. Stelzer, and T. A. Rapoport
Targeting of Rough Endoplasmic Reticulum Membrane Proteins and Ribosomes in Invertebrate Neurons
Mol. Biol. Cell, May 1, 2002; 13(5): 1778 - 1791.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. K. Chatterjee, M. Vashishtha, and M. Kielian
Biochemical Consequences of a Mutation That Controls the Cholesterol Dependence of Semliki Forest Virus Fusion
J. Virol., February 15, 2000; 74(4): 1623 - 1631.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
N. J. Bryant and T. H. Stevens
Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole
Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 230 - 247.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]