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Vol. 8, Issue 11, 2145-2155, November 1997
Departments of Chemistry and Biochemistry, University of Nebraska,
Lincoln, Nebraska 68588
We have used the ciliate Euplotes to study the role
of DNA polymerase in telomeric C strand synthesis.
Euplotes provides a unique opportunity to study C strand
synthesis without the complication of simultaneous DNA replication
because millions of new telomeres are made at a stage in the life cycle
when no general DNA replication takes place. Previously we showed that
the C-strands of newly synthesized telomeres have a precisely
controlled length while the G-strands are more heterogeneous. This
finding suggested that, although synthesis of the G-strand (by
telomerase) is the first step in telomere addition, a major regulatory
step occurs during subsequent C strand synthesis. We have now examined
whether G- and C strand synthesis might be regulated coordinately
rather than by two independent mechanisms. We accomplished this by
determining what happens to G- and C strand length if C strand
synthesis is partially inhibited by aphidicolin. Aphidicolin treatment
caused a general lengthening of the G-strands and a large increase in C
strand heterogeneity. This concomitant change in both the G- and C
strand length indicates that synthesis of the two strands is
coordinated. Since aphidicolin is a very specific inhibitor of DNA
pol
and pol
, our results suggest that this coordinate length
regulation is mediated by DNA polymerase.
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