![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 8, Issue 11, 2307-2327, November 1997
Department of Biology, Division of Cellular and Molecular Medicine,
Howard Hughes Medical Institute, University of California, San Diego,
School of Medicine, La Jolla, California 92093-0668
Protein transport to the lysosome-like vacuole in yeast is mediated
by multiple pathways, including the biosynthetic routes for vacuolar
hydrolases, the endocytic pathway, and autophagy. Among the more than
40 genes required for vacuolar protein sorting (VPS) in
Saccharomyces cerevisiae, mutations in the four class C
VPS genes result in the most severe vacuolar protein
sorting and morphology defects. Herein, we provide complementary
genetic and biochemical evidence that the class C VPS
gene products (Vps18p, Vps11p, Vps16p, and Vps33p) physically and
functionally interact to mediate a late step in protein transport to
the vacuole. Chemical cross-linking experiments demonstrated that
Vps11p and Vps18p, which both contain RING finger zinc-binding domains,
are components of a hetero-oligomeric protein complex that includes
Vps16p and the Sec1p homologue Vps33p. The class C Vps protein complex
colocalized with vacuolar membranes and a distinct dense membrane
fraction. Analysis of cells harboring a temperature-conditional
vps18 allele (vps18tsf) indicated
that Vps18p function is required for the biosynthetic, endocytic, and
autophagic protein transport pathways to the vacuole. In addition,
vps18tsf cells accumulated multivesicular
bodies, autophagosomes, and other membrane compartments that appear to
represent blocked transport intermediates. Overproduction of either
Vps16p or the vacuolar syntaxin homologue Vam3p suppressed defects
associated with vps18tsf mutant cells,
indicating that the class C Vps proteins and Vam3p may functionally
interact. Thus we propose that the class C Vps proteins are components
of a hetero-oligomeric protein complex that mediates the delivery of
multiple transport intermediates to the vacuole.
This article has been cited by other articles:
|
|
 |
|
  H. B. Schonthaler, V. C. Fleisch, O. Biehlmaier, Y. Makhankov, O. Rinner, R. Bahadori, R. Geisler, H. Schwarz, S. C. F. Neuhauss, and R. Dahm The zebrafish mutant lbk/vam6 resembles human multisystemic disorders caused by aberrant trafficking of endosomal vesicles Development, January 15, 2008; 135(2): 387 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shestakova, E. Suvorova, O. Pavliv, G. Khaidakova, and V. Lupashin Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability J. Cell Biol., December 17, 2007; 179(6): 1179 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Filimonenko, S. Stuffers, C. Raiborg, A. Yamamoto, L. Malerod, E. M.C. Fisher, A. Isaacs, A. Brech, H. Stenmark, and A. Simonsen Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease J. Cell Biol., November 5, 2007; 179(3): 485 - 500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Efe, R. J. Botelho, and S. D. Emr Atg18 Regulates Organelle Morphology and Fab1 Kinase Activity Independent of Its Membrane Recruitment by Phosphatidylinositol 3,5-Bisphosphate Mol. Biol. Cell, November 1, 2007; 18(11): 4232 - 4244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Zurita-Martinez, R. Puria, X. Pan, J. D. Boeke, and M. E. Cardenas Efficient Tor Signaling Requires a Functional Class C Vps Protein Complex in Saccharomyces cerevisiae Genetics, August 1, 2007; 176(4): 2139 - 2150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Simonsen, R. C. Cumming, K. Lindmo, V. Galaviz, S. Cheng, T. E. Rusten, and K. D. Finley Genetic Modifiers of the Drosophila Blue Cheese Gene Link Defects in Lysosomal Transport With Decreased Life Span and Altered Ubiquitinated-Protein Profiles Genetics, June 1, 2007; 176(2): 1283 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ohnuki, S. Nogami, H. Kanai, D. Hirata, Y. Nakatani, S. Morishita, and Y. Ohya Diversity of Ca2+-Induced Morphology Revealed by Morphological Phenotyping of Ca2+-Sensitive Mutants of Saccharomyces cerevisiae Eukaryot. Cell, May 1, 2007; 6(5): 817 - 830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Togneri, Y.-S. Cheng, M. Munson, F. M. Hughson, and C. M. Carr Specific SNARE complex binding mode of the Sec1/Munc-18 protein, Sec1p PNAS, November 21, 2006; 103(47): 17730 - 17735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Grosshans, D. Ortiz, and P. Novick Rabs and their effectors: Achieving specificity in membrane traffic PNAS, August 8, 2006; 103(32): 11821 - 11827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Matthews, N. Plumb-Rudewiez, K. Lorent, P. Gissen, C. A. Johnson, F. Lemaigre, and M. Pack Zebrafish vps33b, an ortholog of the gene responsible for human arthrogryposis-renal dysfunction-cholestasis syndrome, regulates biliary development downstream of the onecut transcription factor hnf6 Development, December 1, 2005; 132(23): 5295 - 5306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Palmer, M. N. Kelly, and J. E. Sturtevant The Candida albicans Vacuole Is Required for Differentiation and Efficient Macrophage Killing Eukaryot. Cell, October 1, 2005; 4(10): 1677 - 1686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pulipparacharuvil, M. A. Akbar, S. Ray, E. A. Sevrioukov, A. S. Haberman, J. Rohrer, and H. Kramer Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules J. Cell Sci., August 15, 2005; 118(16): 3663 - 3673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Hermann, L. K. Schroeder, C. A. Hieb, A. M. Kershner, B. M. Rabbitts, P. Fonarev, B. D. Grant, and J. R. Priess Genetic Analysis of Lysosomal Trafficking in Caenorhabditis elegans Mol. Biol. Cell, July 1, 2005; 16(7): 3273 - 3288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gissen, C. A. Johnson, D. Gentle, L. D. Hurst, A. J. Doherty, C. J. O'Kane, D. A. Kelly, and E. R. Maher Comparative evolutionary analysis of VPS33 homologues: genetic and functional insights Hum. Mol. Genet., May 15, 2005; 14(10): 1261 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. LaGrassa and C. Ungermann The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex J. Cell Biol., January 31, 2005; 168(3): 401 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Boyd, T. Hughes, M. Pypaert, and P. Novick Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p J. Cell Biol., December 6, 2004; 167(5): 889 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Meiling-Wesse, H. Barth, C. Voss, E.-L. Eskelinen, U. D. Epple, and M. Thumm Atg21 Is Required for Effective Recruitment of Atg8 to the Preautophagosomal Structure during the Cvt Pathway J. Biol. Chem., September 3, 2004; 279(36): 37741 - 37750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Subramanian, C. A. Woolford, and E. W. Jones The Sec1/Munc18 Protein, Vps33p, Functions at the Endosome and the Vacuole of Saccharomyces cerevisiae Mol. Biol. Cell, June 1, 2004; 15(6): 2593 - 2605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Torroja, N. Gorfinkiel, and I. Guerrero Patched controls the Hedgehog gradient by endocytosis in a dynamin-dependent manner, but this internalization does not play a major role in signal transduction Development, May 15, 2004; 131(10): 2395 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. W. Richardson, S. C. Winistorfer, V. Poupon, J. P. Luzio, and R. C. Piper Mammalian Late Vacuole Protein Sorting Orthologues Participate in Early Endosomal Fusion and Interact with the Cytoskeleton Mol. Biol. Cell, March 1, 2004; 15(3): 1197 - 1210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Poupon, A. Stewart, S. R. Gray, R. C. Piper, and J. P. Luzio The Role of mVps18p in Clustering, Fusion, and Intracellular Localization of Late Endocytic Organelles Mol. Biol. Cell, October 1, 2003; 14(10): 4015 - 4027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Weimer and E. M. Jorgensen Controversies in synaptic vesicle exocytosis J. Cell Sci., September 15, 2003; 116(18): 3661 - 3666. [Full Text] [PDF]
|
|
|
|
|
|
G. E. Palmer, A. Cashmore, and J. Sturtevant Candida albicans VPS11 Is Required for Vacuole Biogenesis and Germ Tube Formation Eukaryot. Cell, June 1, 2003; 2(3): 411 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sriram, K.S. Krishnan, and S. Mayor deep-orange and carnation define distinct stages in late endosomal biogenesis in Drosophila melanogaster J. Cell Biol., May 12, 2003; 161(3): 593 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suzuki, N. Oiso, R. Gautam, E. K. Novak, J.-J. Panthier, P. G. Suprabha, T. Vida, R. T. Swank, and R. A. Spritz The mouse organellar biogenesis mutant buff results from a mutation in Vps33a, a homologue of yeast vps33 and Drosophila carnation PNAS, February 4, 2003; 100(3): 1146 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rojo, J. Zouhar, V. Kovaleva, S. Hong, and N. V. Raikhel The AtC-VPS Protein Complex Is Localized to the Tonoplast and the Prevacuolar Compartment in Arabidopsis Mol. Biol. Cell, February 1, 2003; 14(2): 361 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Desmoucelles, B. Pinson, C. Saint-Marc, and B. Daignan-Fornier Screening the Yeast "Disruptome" for Mutants Affecting Resistance to the Immunosuppressive Drug, Mycophenolic Acid J. Biol. Chem., July 19, 2002; 277(30): 27036 - 27044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Koning, L. L. Larson, E. J. Cadera, M. L. Parrish, and R. L. Wright Mutations That Affect Vacuole Biogenesis Inhibit Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae Genetics, April 1, 2002; 160(4): 1335 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Reggiori and D. J. Klionsky Autophagy in the Eukaryotic Cell Eukaryot. Cell, February 1, 2002; 1(1): 11 - 21. [Full Text] [PDF]
|
|
|
|
|
|
J. R. C. Whyte and S. Munro Vesicle tethering complexes in membrane traffic J. Cell Sci., January 7, 2002; 115(13): 2627 - 2637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Laage and C. Ungermann The N-terminal Domain of the t-SNARE Vam3p Coordinates Priming and Docking in Yeast Vacuole Fusion Mol. Biol. Cell, November 1, 2001; 12(11): 3375 - 3385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ishihara, M. Hamasaki, S. Yokota, K. Suzuki, Y. Kamada, A. Kihara, T. Yoshimori, T. Noda, and Y. Ohsumi Autophagosome Requires Specific Early Sec Proteins for Its Formation and NSF/SNARE for Vacuolar Fusion Mol. Biol. Cell, November 1, 2001; 12(11): 3690 - 3702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Borrelly, J.-C. Boyer, B. Touraine, W. Szponarski, M. Rambier, and R. Gibrat The yeast mutant vps5Delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity PNAS, August 1, 2001; (2001) 161215198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ni and M. Snyder A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2001; 12(7): 2147 - 2170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kim, Y. Kamada, P. E. Stromhaug, J. Guan, A. Hefner-Gravink, M. Baba, S. V. Scott, Y. Ohsumi, W. A. Dunn , Jr., and D. J. Klionsky Cvt9/Gsa9 Functions in Sequestering Selective Cytosolic Cargo Destined for the Vacuole J. Cell Biol., April 16, 2001; 153(2): 381 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kucharczyk, A. M. Kierzek, P. P. Slonimski, and J. Rytka The Ccz1 protein interacts with Ypt7 GTPase during fusion of multiple transport intermediates with the vacuole in S. cerevisiae J. Cell Sci., January 9, 2001; 114(17): 3137 - 3145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Darsow, D. J. Katzmann, C. R. Cowles, and S. D. Emr Vps41p Function in the Alkaline Phosphatase Pathway Requires Homo-oligomerization and Interaction with AP-3 through Two Distinct Domains Mol. Biol. Cell, January 1, 2001; 12(1): 37 - 51. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. J. Klionsky and S. D. Emr Autophagy as a Regulated Pathway of Cellular Degradation Science, December 1, 2000; 290(5497): 1717 - 1721. [Abstract] [Full Text]
|
|
|
|
|
|
A. Wurmser, T. Sato, and S. Emr New Component of the Vacuolar Class C-Vps Complex Couples Nucleotide Exchange on the Ypt7 GTPase to SNARE-dependent Docking and Fusion J. Cell Biol., October 30, 2000; 151(3): 551 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Amerik, J. Nowak, S. Swaminathan, and M. Hochstrasser The Doa4 Deubiquitinating Enzyme Is Functionally Linked to the Vacuolar Protein-sorting and Endocytic Pathways Mol. Biol. Cell, October 1, 2000; 11(10): 3365 - 3380. [Abstract] [Full Text]
|
|
|
|
|
|
A. Srivastava, C. A. Woolford, and E. W. Jones Pep3p/Pep5p Complex: A Putative Docking Factor at Multiple Steps of Vesicular Transport to the Vacuole of Saccharomyces cerevisiae Genetics, September 1, 2000; 156(1): 105 - 122. [Abstract] [Full Text]
|
|
|
|
|
|
L. Jiang, T. E. Phillips, S. W. Rogers, and J. C. Rogers Biogenesis of the Protein Storage Vacuole Crystalloid J. Cell Biol., August 21, 2000; 150(4): 755 - 770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Seals, G. Eitzen, N. Margolis, W. T. Wickner, and A. Price A Ypt/Rab effector complex containing the Sec1 homolog Vps33p is required for homotypic vacuole fusion PNAS, August 15, 2000; 97(17): 9402 - 9407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ungermann, A. Price, and W. Wickner A new role for a SNARE protein as a regulator of the Ypt7/Rab-dependent stage of docking PNAS, July 19, 2000; (2000) 160269997. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. C. DELL’ANGELICA, C. MULLINS, S. CAPLAN, and J. S. BONIFACINO Lysosome-related organelles FASEB J, July 1, 2000; 14(10): 1265 - 1278. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Conboy and M. S. Cyert Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology. Mol. Biol. Cell, July 1, 2000; 11(7): 2429 - 2443. [Abstract] [Full Text]
|
|
|
|
|
|
W. B. Snyder, A. Koller, A. J. Choy, and S. Subramani The Peroxin Pex19p Interacts with Multiple, Integral Membrane Proteins at the Peroxisomal Membrane J. Cell Biol., June 12, 2000; 149(6): 1171 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Luzio, B. Rous, N. Bright, P. Pryor, B. Mullock, and R. Piper Lysosome-endosome fusion and lysosome biogenesis J. Cell Sci., January 5, 2000; 113(9): 1515 - 1524. [Abstract] [PDF]
|
|
|
|
|
|
E. Conibear and T. H. Stevens Vps52p, Vps53p, and Vps54p Form a Novel Multisubunit Complex Required for Protein Sorting at the Yeast Late Golgi Mol. Biol. Cell, January 1, 2000; 11(1): 305 - 323. [Abstract] [Full Text]
|
|
|
|
|
|
W. B. Snyder, A. Koller, A. J. Choy, M. A. Johnson, J. M. Cregg, L. Rangell, G. A. Keller, and S. Subramani Pex17p Is Required for Import of Both Peroxisome Membrane and Lumenal Proteins and Interacts with Pex19p and the Peroxisome Targeting Signal-Receptor Docking Complex in Pichia pastoris Mol. Biol. Cell, December 1, 1999; 10(12): 4005 - 4019. [Abstract] [Full Text]
|
|
|
|
|
|
T. Vida and B. Gerhardt A Cell-free Assay Allows Reconstitution of Vps33p-dependent Transport to the Yeast Vacuole/Lysosome J. Cell Biol., July 12, 1999; 146(1): 85 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koller, W. B. Snyder, K. N. Faber, T. J. Wenzel, L. Rangell, G. A. Keller, and S. Subramani Pex22p of Pichia pastoris, Essential for Peroxisomal Matrix Protein Import, Anchors the Ubiquitin-conjugating Enzyme, Pex4p, on the Peroxisomal Membrane J. Cell Biol., July 12, 1999; 146(1): 99 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. von Mollard and T. H. Stevens The Saccharomyces cerevisiae v-SNARE Vti1p Is Required for Multiple Membrane Transport Pathways to the Vacuole Mol. Biol. Cell, June 1, 1999; 10(6): 1719 - 1732. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. Nierras and J. R. Warner Protein Kinase C Enables the Regulatory Circuit That Connects Membrane Synthesis to Ribosome Synthesis in Saccharomyces cerevisiae J. Biol. Chem., May 7, 1999; 274(19): 13235 - 13241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, T. Kane, C. Tipper, P. Spatrick, and D. D. Jenness Yeast Mutants Affecting Possible Quality Control of Plasma Membrane Proteins Mol. Cell. Biol., May 1, 1999; 19(5): 3588 - 3599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Miseta, L. Fu, R. Kellermayer, J. Buckley, and D. M. Bedwell The Golgi Apparatus Plays a Significant Role in the Maintenance of Ca2+ Homeostasis in the vps33Delta Vacuolar Biogenesis Mutant of Saccharomyces cerevisiae J. Biol. Chem., February 26, 1999; 274(9): 5939 - 5947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Simmen, A Schmidt, W Hunziker, and F Beermann The tyrosinase tail mediates sorting to the lysosomal compartment in MDCK cells via a di-leucine and a tyrosine-based signal J. Cell Sci., January 1, 1999; 112(1): 45 - 53. [Abstract] [PDF]
|
|
|
|
|
|
T. K. Sato, T. Darsow, and S. D. Emr Vam7p, a SNAP-25-Like Molecule, and Vam3p, a Syntaxin Homolog, Function Together in Yeast Vacuolar Protein Trafficking Mol. Cell. Biol., September 1, 1998; 18(9): 5308 - 5319. [Abstract] [Full Text]
|
|
|
|
|
|
B. Gerhardt, T. J. Kordas, C. M. Thompson, P. Patel, and T. Vida The Vesicle Transport Protein Vps33p Is an ATP-binding Protein That Localizes to the Cytosol in an Energy-dependent Manner J. Biol. Chem., June 19, 1998; 273(25): 15818 - 15829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Klionsky Nonclassical Protein Sorting to the Yeast Vacuole J. Biol. Chem., May 1, 1998; 273(18): 10807 - 10810. [Full Text] [PDF]
|
|
|
|
|
|
B. Y. Kim, H. Kramer, A. Yamamoto, E. Kominami, S. Kohsaka, and C. Akazawa Molecular Characterization of Mammalian Homologues of Class C Vps Proteins That Interact with Syntaxin-7 J. Biol. Chem., July 27, 2001; 276(31): 29393 - 29402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ungermann, A. Price, and W. Wickner A new role for a SNARE protein as a regulator of the Ypt7/Rab-dependent stage of docking PNAS, August 1, 2000; 97(16): 8889 - 8891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
