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Vol. 8, Issue 12, 2421-2436, December 1997
Department of Molecular, Cellular, and Developmental Biology,
University of Colorado, Boulder, Colorado 80309-0347
The trithorax gene family contains members
implicated in the control of transcription, development, chromosome
structure, and human leukemia. A feature shared by some family members,
and by other proteins that function in chromatin-mediated
transcriptional regulation, is the presence of a 130- to 140-amino acid
motif dubbed the SET or Tromo domain. Here we present analysis of
SET1, a yeast member of the trithorax
gene family that was identified by sequence inspection to encode a
1080-amino acid protein with a C-terminal SET domain. In addition to
its SET domain, which is 40-50% identical to those previously
characterized, SET1 also shares dispersed but
significant similarity to Drosophila and human
trithorax homologues. To understand SET1
function(s), we created a null mutant. Mutant strains, although viable,
are defective in transcriptional silencing of the silent mating-type
loci and telomeres. The telomeric silencing defect is rescued not only by full-length episomal SET1 but also by the conserved
SET domain of SET1. set1 mutant strains display other
phenotypes including morphological abnormalities, stationary phase
defects, and growth and sporulation defects. Candidate genes that may
interact with SET1 include those with functions in
transcription, growth, and cell cycle control. These data suggest that
yeast SET1, like its SET domain counterparts in other
organisms, functions in diverse biological processes including
transcription and chromatin structure.
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