![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
E David, JB McNeil, V Basile and RE Pearlman
Department of Biology, York University, Toronto, Ontario, Canada.
The diploid germinal nucleus of the ciliated protozoan Tetrahymena thermophila is unusual among eukaryotes in that it encodes a single copy of the gene for rRNA allowing identification of cis-acting mutations in rDNA affecting rRNA structure, function, and processing. The generally conserved nucleolar protein fibrillarin has been characterized from a number of systems and is involved in pre-rRNA processing. We have demonstrated that Tetrahymena has fibrillarin and have analyzed the cDNA and the genomic DNA encoding this protein. The derived amino acid sequence of the N-terminal region of Tetrahymena fibrillarin shows little similarity with the generally highly conserved glycine/arginine-rich N-terminal domain of other eukaryotic fibrillarins. The remainder of the amino acid sequence of the molecule is more conserved. Polyclonal antibodies generated against the full- length Tetrahymena fibrillarin expressed in bacteria recognize a protein of M(r) approximately 32,000 in whole-cell or nucleolar preparations. Immunocytochemistry localizes fibrillarin to nucleoli in the somatic macronuclei of vegetative cells. Transformation experiments demonstrate that fibrillarin is an essential protein in Tetrahymena. The Tetrahymena fibrillarin is expressed but does not complement a NOP1 null mutation when transformed into the yeast Saccharomyces cerevisiae, indicating less functional conservation among fibrillarins than previously suggested.
This article has been cited by other articles:
|
|
 |
|
  J. S. Fillingham, J. Garg, N. Tsao, N. Vythilingum, T. Nishikawa, and R. E. Pearlman Molecular Genetic Analysis of an SNF2/brahma-Related Gene in Tetrahymena thermophila Suggests Roles in Growth and Nuclear Development. Eukaryot. Cell, August 1, 2006; 5(8): 1347 - 1359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Russell, Y.-i. Watanabe, J. M. Charette, and M. W. Gray Unusual features of fibrillarin cDNA and gene structure in Euglena gracilis: evolutionary conservation of core proteins and structural predictions for methylation-guide box C/D snoRNPs throughout the domain Eucarya Nucleic Acids Res., May 13, 2005; 33(9): 2781 - 2791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yanagida, T. Hayano, Y. Yamauchi, T. Shinkawa, T. Natsume, T. Isobe, and N. Takahashi Human Fibrillarin Forms a Sub-complex with Splicing Factor 2-associated p32, Protein Arginine Methyltransferases, and Tubulins {alpha}3 and {beta}1 That Is Independent of Its Association with Preribosomal Ribonucleoprotein Complexes J. Biol. Chem., January 16, 2004; 279(3): 1607 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Snaar, Wiesmeijer, A. Jochemsen, H. Tanke, and R. Dirks Mutational Analysis of Fibrillarin and Its Mobility in Living Human Cells J. Cell Biol., October 30, 2000; 151(3): 653 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Pih, M. J. Yi, Y. S. Liang, B. J. Shin, M. J. Cho, I. Hwang, and D. Son Molecular Cloning and Targeting of a Fibrillarin Homolog from Arabidopsis Plant Physiology, May 1, 2000; 123(1): 51 - 58. [Abstract] [Full Text]
|
|
|
|
 |
|
 N Fomproix, J Gebrane-Younes, and D Hernandez-Verdun Effects of anti-fibrillarin antibodies on building of functional nucleoli at the end of mitosis J. Cell Sci., January 2, 1998; 111(3): 359 - 372. [Abstract] [PDF]
|
|
|
|
|
|
D. A. Dunbar, S. Wormsley, T. M. Lowe, and S. J. Baserga Fibrillarin-associated Box C/D Small Nucleolar RNAs in Trypanosoma brucei. SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2'-O-RIBOSE METHYLATION OF rRNA J. Biol. Chem., May 5, 2000; 275(19): 14767 - 14776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Barneche, F. Steinmetz, and M. Echeverria Fibrillarin Genes Encode Both a Conserved Nucleolar Protein and a Novel Small Nucleolar RNA Involved in Ribosomal RNA Methylation in Arabidopsis thaliana J. Biol. Chem., August 25, 2000; 275(35): 27212 - 27220. [Abstract] [Full Text] [PDF]
|
|
