![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
R Verma, RM Feldman and RJ Deshaies
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Traversal from G1 to S-phase in cycling cells of budding yeast is dependent on the destruction of the S-phase cyclin/CDK inhibitor SIC1. Genetic data suggest that SIC1 proteolysis is mediated by the ubiquitin pathway and requires the action of CDC34, CDC4, CDC53, SKP1, and CLN/CDC28. As a first step in defining the functions of the corresponding gene products, we have reconstituted SIC1 multiubiquitination in DEAE-fractionated yeast extract. Multiubiquitination depends on cyclin/CDC28 protein kinase and the CDC34 ubiquitin-conjugating enzyme. Ubiquitin chain formation is abrogated in cdc4ts mutant extracts and assembly restored by the addition of exogenous CDC4, suggesting a direct role for this protein in SIC1 multiubiquitination. Deletion analysis of SIC1 indicates that the N-terminal 160 residues are both necessary and sufficient to serve as substrate for CDC34-dependent ubiquitination. The complementary C- terminal segment of SIC1 binds to the S-phase cyclin CLB5, indicating a modular structure for SIC1.
This article has been cited by other articles:
|
|
 |
|
  W. Jonkers and M. Rep Lessons from Fungal F-Box Proteins Eukaryot. Cell, May 1, 2009; 8(5): 677 - 695. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mittag, S. Orlicky, W.-Y. Choy, X. Tang, H. Lin, F. Sicheri, L. E. Kay, M. Tyers, and J. D. Forman-Kay Dynamic equilibrium engagement of a polyvalent ligand with a single-site receptor PNAS, November 18, 2008; 105(46): 17772 - 17777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. R. Cross, L. Schroeder, and J. M. Bean Phosphorylation of the Sic1 Inhibitor of B-Type Cyclins in Saccharomyces cerevisiae Is Not Essential but Contributes to Cell Cycle Robustness Genetics, July 1, 2007; 176(3): 1541 - 1555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Honey and B. Futcher Roles of the CDK Phosphorylation Sites of Yeast Cdc6 in Chromatin Binding and Rereplication Mol. Biol. Cell, April 1, 2007; 18(4): 1324 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Pal, N. C. Chan, L. Helfenbaum, K. Tan, W. P. Tansey, and M.-J. Gething SCFCdc4-mediated Degradation of the Hac1p Transcription Factor Regulates the Unfolded Protein Response in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2007; 18(2): 426 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Catlett and K. B. Kaplan Sgt1p Is a Unique Co-chaperone That Acts as a Client Adaptor to Link Hsp90 to Skp1p J. Biol. Chem., November 3, 2006; 281(44): 33739 - 33748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. P. Jackson, S. I. Reed, and S. B. Haase Distinct mechanisms control the stability of the related s-phase cyclins clb5 and clb6. Mol. Cell. Biol., March 1, 2006; 26(6): 2456 - 2466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Santangelo Glucose Signaling in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 253 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-C. Chuang, X.-N. Zhu, C. R. Herrera, H.-M. Tseng, C. M. Pfleger, K. Block, and P. R. Yew The C-terminal Domain of the Xenopus Cyclin-dependent Kinase Inhibitor, p27Xic1, Is Both Necessary and Sufficient for Phosphorylation-independent Proteolysis J. Biol. Chem., October 21, 2005; 280(42): 35290 - 35298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, M. Spirek, J. Thornton, and R. A. Butow A Novel Degron-mediated Degradation of the RTG Pathway Regulator, Mks1p, by SCFGrr1 Mol. Biol. Cell, October 1, 2005; 16(10): 4893 - 4904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Atir-Lande, T. Gildor, and D. Kornitzer Role for the SCFCDC4 Ubiquitin Ligase in Candida albicans Morphogenesis Mol. Biol. Cell, June 1, 2005; 16(6): 2772 - 2785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Y. Su, K. Flick, and P. Kaiser The F-Box Protein Met30 Is Required for Multiple Steps in the Budding Yeast Cell Cycle Mol. Cell. Biol., May 15, 2005; 25(10): 3875 - 3885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chauhan, T. Hideshima, C. Mitsiades, P. Richardson, and K. C. Anderson Proteasome inhibitor therapy in multiple myeloma Mol. Cancer Ther., April 1, 2005; 4(4): 686 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Arnason, M. G. Pisclevich, M. D. Dash, G. F. Davies, and T. A. A. Harkness Novel Interaction between Apc5p and Rsp5p in an Intracellular Signaling Pathway in Saccharomyces cerevisiae Eukaryot. Cell, January 1, 2005; 4(1): 134 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Chen, L. Calzone, A. Csikasz-Nagy, F. R. Cross, B. Novak, and J. J. Tyson Integrative Analysis of Cell Cycle Control in Budding Yeast Mol. Biol. Cell, August 1, 2004; 15(8): 3841 - 3862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Berset, P. Griac, R. Tempel, J. La Rue, C. Wittenberg, and S. Lanker Transferable Domain in the G1 Cyclin Cln2 Sufficient To Switch Degradation of Sic1 from the E3 Ubiquitin Ligase SCFCdc4 to SCFGrr1 Mol. Cell. Biol., July 1, 2002; 22(13): 4463 - 4476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. You, K. Harvey, L. Kong, and J. Newport Xic1 degradation in Xenopus egg extracts is coupled to initiation of DNA replication Genes & Dev., May 15, 2002; 16(10): 1182 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Sclafani, M. Tecklenburg, and A. Pierce The mcm5-bob1 Bypass of Cdc7p/Dbf4p in DNA Replication Depends on Both Cdk1-Independent and Cdk1-Dependent Steps in Saccharomyces cerevisiae Genetics, May 1, 2002; 161(1): 47 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Furuchi, G.-W. Hwang, and A. Naganuma Overexpression of the Ubiquitin-Conjugating Enzyme Cdc34 Confers Resistance to Methylmercury in Saccharomyces cerevisiae Mol. Pharmacol., April 1, 2002; 61(4): 738 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Cross, V. Archambault, M. Miller, and M. Klovstad Testing a Mathematical Model of the Yeast Cell Cycle Mol. Biol. Cell, January 1, 2002; 13(1): 52 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chi, M. J. Huddleston, X. Zhang, R. A. Young, R. S. Annan, S. A. Carr, and R. J. Deshaies Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase Genes & Dev., May 1, 2001; 15(9): 1078 - 1092. [Abstract] [Full Text]
|
|
|
|
|
|
Y. G. Hsiung, H.-C. Chang, J.-L. Pellequer, R. La Valle, S. Lanker, and C. Wittenberg F-Box Protein Grr1 Interacts with Phosphorylated Targets via the Cationic Surface of Its Leucine-Rich Repeat Mol. Cell. Biol., April 1, 2001; 21(7): 2506 - 2520. [Abstract] [Full Text]
|
|
|
|
|
|
K. Asakawa, S. Yoshida, F. Otake, and A. Toh-e A Novel Functional Domain of Cdc15 Kinase Is Required for Its Interaction With Tem1 GTPase in Saccharomyces cerevisiae Genetics, April 1, 2001; 157(4): 1437 - 1450. [Abstract] [Full Text]
|
|
|
|
|
|
C. Swanson, J. Ross, and P. K. Jackson Nuclear accumulation of cyclin E/Cdk2 triggers a concentration-dependent switch for the destruction of p27Xic1 PNAS, July 5, 2000; 97(14): 7796 - 7801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. R. Cross and M. D. Jacobson Conservation and Function of a Potential Substrate-Binding Domain in the Yeast Clb5 B-Type Cyclin Mol. Cell. Biol., July 1, 2000; 20(13): 4782 - 4790. [Abstract] [Full Text]
|
|
|
|
|
|
S. Irniger, M. Bäumer, and G. H. Braus Glucose and Ras Activity Influence the Ubiquitin Ligases APC/C and SCF in Saccharomyces cerevisiae Genetics, April 1, 2000; 154(4): 1509 - 1521. [Abstract] [Full Text]
|
|
|
|
|
|
L. Li, M. Ljungman, and J. E. Dixon The Human Cdc14 Phosphatases Interact with and Dephosphorylate the Tumor Suppressor Protein p53 J. Biol. Chem., January 28, 2000; 275(4): 2410 - 2414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Reymond, C Wirbelauer, and W Krek Association of human ubiquitin-conjugating enzyme CDC34 with the mitotic spindle in anaphase J. Cell Sci., January 5, 2000; 113(10): 1687 - 1694. [Abstract] [PDF]
|
|
|
|
|
|
S. Elsasser, Y. Chi, P. Yang, and J. L. Campbell Phosphorylation Controls Timing of Cdc6p Destruction: A Biochemical Analysis Mol. Biol. Cell, October 1, 1999; 10(10): 3263 - 3277. [Abstract] [Full Text]
|
|
|
|
|
|
X. Li and M. Cai Recovery of the Yeast Cell Cycle from Heat Shock-induced G1 Arrest Involves a Positive Regulation of G1 Cyclin Expression by the S Phase Cyclin Clb5 J. Biol. Chem., August 20, 1999; 274(34): 24220 - 24231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pasero, B. P. Duncker, E. Schwob, and S. M. Gasser A role for the Cdc7 kinase regulatory subunit Dbf4p in the formation of initiation-competent origins of replication Genes & Dev., August 15, 1999; 13(16): 2159 - 2176. [Abstract] [Full Text]
|
|
|
|
|
|
J. H. Seol, R.M. R. Feldman, W. Zachariae, A. Shevchenko, C. C. Correll, S. Lyapina, Y. Chi, M. Galova, J. Claypool, S. Sandmeyer, et al. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34 Genes & Dev., June 15, 1999; 13(12): 1614 - 1626. [Abstract] [Full Text]
|
|
|
|
|
|
T Kishi and F Yamao An essential function of Grr1 for the degradation of Cln2 is to act as a binding core that links Cln2 to Skp1 J. Cell Sci., June 14, 1999; 111(24): 3655 - 3661. [Abstract] [PDF]
|
|
|
|
 |
|
 I. D. Russell, A. S. Grancell, and P. K. Sorger The Unstable F-box Protein p58-Ctf13 Forms the Structural Core of the CBF3 Kinetochore Complex J. Cell Biol., May 31, 1999; 145(5): 933 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kamura, D. M. Koepp, M. N. Conrad, D. Skowyra, R. J. Moreland, O. Iliopoulos, W. S. Lane, W. G. Kaelin Jr., S. J. Elledge, R. C. Conaway, et al. Rbx1, a Component of the VHL Tumor Suppressor Complex and SCF Ubiquitin Ligase Science, April 23, 1999; 284(5414): 657 - 661. [Abstract] [Full Text]
|
|
|
|
|
|
J. T. Winston, P. Strack, P. Beer-Romero, C. Y. Chu, S. J. Elledge, and J. W. Harper The SCFbeta -TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in Ikappa Balpha and beta -catenin and stimulates Ikappa Balpha ubiquitination in vitro Genes & Dev., February 1, 1999; 13(3): 270 - 283. [Abstract] [Full Text]
|
|
|
|
|
|
M. D. Mendenhall and A. E. Hodge Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1191 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishizawa, M. Kawasumi, M. Fujino, and A. Toh-e Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation Mol. Biol. Cell, September 1, 1998; 9(9): 2393 - 2405. [Abstract] [Full Text]
|
|
|
|
|
|
P. Kaiser, R. A.L. Sia, E. G.S. Bardes, D. J. Lew, and S. I. Reed Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1 Genes & Dev., August 15, 1998; 12(16): 2587 - 2597. [Abstract] [Full Text]
|
|
|
|
|
|
D. Lammer, N. Mathias, J. M. Laplaza, W. Jiang, Y. Liu, J. Callis, M. Goebl, and M. Estelle Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCFCdc4 complex Genes & Dev., April 1, 1998; 12(7): 914 - 926. [Abstract] [Full Text]
|
|
|
|
|
|
E. E. Patton, A. R. Willems, D. Sa, L. Kuras, D. Thomas, K. L. Craig, and M. Tyers Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast Genes & Dev., March 1, 1998; 12(5): 692 - 705. [Abstract] [Full Text]
|
|
|
|
|
|
A Sanchez-Diaz, I Gonzalez, M Arellano, and S Moreno The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast J. Cell Sci., January 3, 1998; 111(6): 843 - 851. [Abstract] [PDF]
|
|
|
|
|
|
S. Henchoz, Y. Chi, B. Catarin, I. Herskowitz, R. J. Deshaies, and M. Peter Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast Genes & Dev., November 15, 1997; 11(22): 3046 - 3060. [Abstract] [Full Text] [PDF]
|
|
