![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
BF Horazdovsky, BA Davies, MN Seaman, SA McLaughlin, S Yoon and SD Emr
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9038, USA.
A number of the Saccharomyces cerevisiae vacuolar protein-sorting (vps) mutants exhibit an altered vacuolar morphology. Unlike wild-type cells that contain 1-3 large vacuolar structures, the class B vps5 and vps17 mutant cells contain 10-20 smaller vacuole-like compartments. To explore the role of these VPS gene products in vacuole biogenesis, we cloned and sequenced VPS5 and characterized its protein products. The VPS5 gene is predicted to encode a very hydrophilic protein of 675 amino acids that shows significant sequence homology with mammalian sorting nexin-1. Polyclonal antiserum directed against the VPS5 gene product detects a single, cytoplasmic protein that is phosphorylated specifically on a serine residue(s). Subcellular fractionation studies indicate that Vps5p is associated peripherally with a dense membrane fraction distinct from Golgi, endosomal, and vacuolar membranes. This association was found to be dependent on the presence of another class B VPS gene product, Vps17p. Biochemical cross-linking studies demonstrated that Vps5p and Vps17p physically interact. Gene disruption experiments show that the VPS5 genes product is not essential for cell viability; however, cells carrying the null allele contain fragmented vacuoles and exhibit defects in vacuolar protein-sorting similar to vps17 null mutants. More than 95% of carboxypeptidase Y is secreted from these cells in its Golgi-modified p2 precursor form. Additionally, the Vps10p vacuolar protein-sorting receptor is mislocalized to the vacuole in vps5 mutant cells. On the basis of these and other observations, we propose that the Vps17p protein complex may participate in the intracellular trafficking of the Vps10p-sorting receptor, as well as other later-Golgi proteins.
This article has been cited by other articles:
|
|
 |
|
  N. Q. Phan, S.-J. Kim, and D. C. Bassham Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole Mol Plant, October 3, 2008; (2008) ssn057v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hara, E. Kiyokawa, S.-i. Iemura, T. Natsume, T. Wassmer, P. J. Cullen, H. Hiai, and M. Matsuda The DHR1 Domain of DOCK180 Binds to SNX5 and Regulates Cation-independent Mannose 6-phosphate Receptor Transport Mol. Biol. Cell, September 1, 2008; 19(9): 3823 - 3835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Onishi, M. Iida, T. Koga, S. Yamada, A. Hirata, T. Iwaki, K. Takegawa, Y. Fukui, and H. Tachikawa Schizosaccharomyces pombe Sst4p, a Conserved Vps27/Hrs Homolog, Functions Downstream of Phosphatidylinositol 3-Kinase Pik3p To Mediate Proper Spore Formation Eukaryot. Cell, December 1, 2007; 6(12): 2343 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. I. Strochlic, T. G. Setty, A. Sitaram, and C. G. Burd Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling J. Cell Biol., April 9, 2007; 177(1): 115 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Rojas, S. Kametaka, C. R. Haft, and J. S. Bonifacino Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors Mol. Cell. Biol., February 1, 2007; 27(3): 1112 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Qin, M. He, X. Chen, and D. Pei Sorting Nexin 10 Induces Giant Vacuoles in Mammalian Cells J. Biol. Chem., December 1, 2006; 281(48): 36891 - 36896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shimada, Y. Koumoto, L. Li, M. Yamazaki, M. Kondo, M. Nishimura, and I. Hara-Nishimura AtVPS29, a Putative Component of a Retromer Complex, is Required for the Efficient Sorting of Seed Storage Proteins Plant Cell Physiol., September 1, 2006; 47(9): 1187 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Y. M. Coudreuse, G. Roel, M. C. Betist, O. Destree, and H. C. Korswagen Wnt Gradient Formation Requires Retromer Function in Wnt-Producing Cells Science, May 12, 2006; 312(5775): 921 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Iwaki, A. Hosomi, S. Tokudomi, Y. Kusunoki, Y. Fujita, Y. Giga-Hama, N. Tanaka, and K. Takegawa Vacuolar protein sorting receptor in Schizosaccharomyces pombe. Microbiology, May 1, 2006; 152(Pt 5): 1523 - 1532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Oliviusson, O. Heinzerling, S. Hillmer, G. Hinz, Y. C. Tse, L. Jiang, and D. G. Robinson Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors PLANT CELL, May 1, 2006; 18(5): 1239 - 1252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gullapalli, B. L. Wolfe, C. T. Griffin, T. Magnuson, and J. Trejo An Essential Role for SNX1 in Lysosomal Sorting of Protease-activated Receptor-1: Evidence for Retromer-, Hrs-, and Tsg101-independent Functions of Sorting Nexins Mol. Biol. Cell, March 1, 2006; 17(3): 1228 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Carlton, M. V. Bujny, B. J. Peter, V. M. J. Oorschot, A. Rutherford, R. S. Arkell, J. Klumperman, H. T. McMahon, and P. J. Cullen Sorting nexin-2 is associated with tubular elements of the early endosome, but is not essential for retromer-mediated endosome-to-TGN transport J. Cell Sci., October 1, 2005; 118(19): 4527 - 4539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wang, M. Guo, Z. Liang, J. Fan, Z. Zhu, J. Zang, Z. Zhu, X. Li, M. Teng, L. Niu, et al. Crystal Structure of Human Vacuolar Protein Sorting Protein 29 Reveals a Phosphodiesterase/Nuclease-like Fold and Two Protein-Protein Interaction Sites J. Biol. Chem., June 17, 2005; 280(24): 22962 - 22967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bruinsma, R. G. Spelbrink, and S. F. Nothwehr Retrograde Transport of the Mannosyltransferase Och1p to the Early Golgi Requires a Component of the COG Transport Complex J. Biol. Chem., September 17, 2004; 279(38): 39814 - 39823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Choi, W.-P. Hong, M. J. Kim, J. H. Kim, S. H. Ryu, and P.-G. Suh Sorting nexin 16 regulates EGF receptor trafficking by phosphatidylinositol-3-phosphate interaction with the Phox domain J. Cell Sci., August 15, 2004; 117(18): 4209 - 4218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. R. Parrish, C. J. Stefan, and S. D. Emr Essential Role for the Myotubularin-related Phosphatase Ymr1p and the Synaptojanin-like Phosphatases Sjl2p and Sjl3p in Regulation of Phosphatidylinositol 3-Phosphate in Yeast Mol. Biol. Cell, August 1, 2004; 15(8): 3567 - 3579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Roth Phosphoinositides in Constitutive Membrane Traffic Physiol Rev, July 1, 2004; 84(3): 699 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gullapalli, T. A. Garrett, M. M. Paing, C. T. Griffin, Y. Yang, and J. Trejo A Role for Sorting Nexin 2 in Epidermal Growth Factor Receptor Down-regulation: Evidence for Distinct Functions of Sorting Nexin 1 and 2 in Protein Trafficking Mol. Biol. Cell, May 1, 2004; 15(5): 2143 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Burden, X.-M. Sun, A. B. G. Garcia, and A. K. Soutar Sorting Motifs in the Intracellular Domain of the Low Density Lipoprotein Receptor Interact with a Novel Domain of Sorting Nexin-17 J. Biol. Chem., April 16, 2004; 279(16): 16237 - 16245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. N. Arighi, L. M. Hartnell, R. C. Aguilar, C. R. Haft, and J. S. Bonifacino Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor J. Cell Biol., April 12, 2004; 165(1): 123 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pons, F. Hullin-Matsuda, M. Nauze, R. Barbaras, C. Peres, X. Collet, B. Perret, H. Chap, and A. Gassama-Diagne Enterophilin-1, a New Partner of Sorting Nexin 1, Decreases Cell Surface Epidermal Growth Factor Receptor J. Biol. Chem., May 30, 2003; 278(23): 21155 - 21161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kato and W. Wickner Vam10p defines a Sec18p-independent step of priming that allows yeast vacuole tethering PNAS, May 27, 2003; 100(11): 6398 - 6403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leprince, E. Le Scolan, B. Meunier, V. Fraisier, N. Brandon, J. De Gunzburg, and J. Camonis Sorting nexin 4 and amphiphysin 2, a new partnership between endocytosis and intracellular trafficking J. Cell Sci., May 15, 2003; 116(10): 1937 - 1948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Burda, S. M. Padilla, S. Sarkar, and S. D. Emr Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase J. Cell Sci., October 15, 2002; 115(20): 3889 - 3900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Schwarz, C. T. Griffin, E. A. Schneider, D. Yee, and T. Magnuson Genetic Analysis of Sorting Nexins 1 and 2 Reveals a Redundant and Essential Function in Mice Mol. Biol. Cell, October 1, 2002; 13(10): 3588 - 3600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N.J. Seaman and H. P. Williams Identification of the Functional Domains of Yeast Sorting Nexins Vps5p and Vps17p Mol. Biol. Cell, August 1, 2002; 13(8): 2826 - 2840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, Y. Zhou, K. Szabo, C. R. Haft, and J. Trejo Down-Regulation of Protease-activated Receptor-1 Is Regulated by Sorting Nexin 1 Mol. Biol. Cell, June 1, 2002; 13(6): 1965 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Lin, C. G. Lo, R. A. Cerione, and W. Yang The Cdc42 Target ACK2 Interacts with Sorting Nexin 9 (SH3PX1) to Regulate Epidermal Growth Factor Receptor Degradation J. Biol. Chem., March 15, 2002; 277(12): 10134 - 10138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Sato, M. Overduin, and S. D. Emr Location, Location, Location: Membrane Targeting Directed by PX Domains Science, November 30, 2001; 294(5548): 1881 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Yu and M. A. Lemmon All Phox Homology (PX) Domains from Saccharomyces cerevisiae Specifically Recognize Phosphatidylinositol 3-Phosphate J. Biol. Chem., November 16, 2001; 276(47): 44179 - 44184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Worby, N. Simonson-Leff, J. C. Clemens, R. P. Kruger, M. Muda, and J. E. Dixon The Sorting Nexin, DSH3PX1, Connects the Axonal Guidance Receptor, Dscam, to the Actin Cytoskeleton J. Biol. Chem., November 2, 2001; 276(45): 41782 - 41789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-A. Ha, J. T. Bunch, H. Hama, D. B. DeWald, and S. F. Nothwehr A Novel Mechanism for Localizing Membrane Proteins to Yeast Trans-Golgi Network Requires Function of Synaptojanin-like Protein Mol. Biol. Cell, October 1, 2001; 12(10): 3175 - 3190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Reddy and M. N.J. Seaman Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval Mol. Biol. Cell, October 1, 2001; 12(10): 3242 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, D. Liu, G. Gill, and Z. Songyang Regulation of cytokine-independent survival kinase (CISK) by the Phox homology domain and phosphoinositides J. Cell Biol., August 20, 2001; 154(4): 699 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Borrelly, J.-C. Boyer, B. Touraine, W. Szponarski, M. Rambier, and R. Gibrat The yeast mutant vps5Delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity PNAS, August 1, 2001; (2001) 161215198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kurten, A. Eddington, P Chowdhury, R. Smith, A. Davidson, and B. Shank Self-assembly and binding of a sorting nexin to sorting endosomes J. Cell Sci., January 5, 2001; 114(9): 1743 - 1756. [Abstract] [PDF]
|
|
|
|
|
|
E. S. Bensen, B. G. Yeung, and G. S. Payne Ric1p and the Ypt6p GTPase Function in a Common Pathway Required for Localization of Trans-Golgi Network Membrane Proteins Mol. Biol. Cell, January 1, 2001; 12(1): 13 - 26. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. Haft, M. d. l. L. Sierra, R. Bafford, M. A. Lesniak, V. A. Barr, and S. I. Taylor Human Orthologs of Yeast Vacuolar Protein Sorting Proteins Vps26, 29, and 35: Assembly into Multimeric Complexes Mol. Biol. Cell, December 1, 2000; 11(12): 4105 - 4116. [Abstract] [Full Text]
|
|
|
|
|
|
S. F. Nothwehr, S.-A. Ha, and P. Bruinsma Sorting of Yeast Membrane Proteins into an Endosome-to-Golgi Pathway Involves Direct Interaction of Their Cytosolic Domains with Vps35p J. Cell Biol., October 18, 2000; 151(2): 297 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Conboy and M. S. Cyert Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology. Mol. Biol. Cell, July 1, 2000; 11(7): 2429 - 2443. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. S. Bensen, G. Costaguta, and G. S. Payne Synthetic Genetic Interactions With Temperature-Sensitive Clathrin in Saccharomyces cerevisiae: Roles for Synaptojanin-Like Inp53p and Dynamin-Related Vps1p in Clathrin-Dependent Protein Sorting at the trans-Golgi Network Genetics, January 1, 2000; 154(1): 83 - 97. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Takemoto, M. Furuta, M. Sato, M. Kubo, and Y. Hashimoto Isolation and characterization of a novel HS1 SH3 domain binding protein, HS1BP3 Int. Immunol., December 1, 1999; 11(12): 1957 - 1964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Corson, J. Folmer, J. J. Strain, V. C. Culotta, and D. W. Cleveland Oxidative Stress and Iron Are Implicated in Fragmenting Vacuoles of Saccharomyces cerevisiae Lacking Cu,Zn-Superoxide Dismutase J. Biol. Chem., September 24, 1999; 274(39): 27590 - 27596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hama, G. G. Tall, and B. F. Horazdovsky Vps9p Is a Guanine Nucleotide Exchange Factor Involved in Vesicle-mediated Vacuolar Protein Transport J. Biol. Chem., May 21, 1999; 274(21): 15284 - 15291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Nothwehr, P. Bruinsma, and L. A. Strawn Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment Mol. Biol. Cell, April 1, 1999; 10(4): 875 - 890. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. Haft, M. de la Luz Sierra, V. A. Barr, D. H. Haft, and S. I. Taylor Identification of a Family of Sorting Nexin Molecules and Characterization of Their Association with Receptors Mol. Cell. Biol., December 1, 1998; 18(12): 7278 - 7287. [Abstract] [Full Text]
|
|
|
|
|
|
T. K. Sato, T. Darsow, and S. D. Emr Vam7p, a SNAP-25-Like Molecule, and Vam3p, a Syntaxin Homolog, Function Together in Yeast Vacuolar Protein Trafficking Mol. Cell. Biol., September 1, 1998; 18(9): 5308 - 5319. [Abstract] [Full Text]
|
|
|
|
|
|
M. N.J. Seaman, J. Michael McCaffery, and S. D. Emr A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast J. Cell Biol., August 10, 1998; 142(3): 665 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Voos and T. H. Stevens Retrieval of Resident Late-Golgi Membrane Proteins from the Prevacuolar Compartment of Saccharomyces cerevisiae Is Dependent on the Function of Grd19p J. Cell Biol., February 9, 1998; 140(3): 577 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-S. Chin, M. C. Raynor, X. Wei, H.-Q. Chen, and L. Li Hrs Interacts with Sorting Nexin 1 and Regulates Degradation of Epidermal Growth Factor Receptor J. Biol. Chem., March 2, 2001; 276(10): 7069 - 7078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Phillips, V. A. Barr, D. H. Haft, S. I. Taylor, and C. R. Haft Identification and Characterization of SNX15, a Novel Sorting Nexin Involved in Protein Trafficking J. Biol. Chem., February 9, 2001; 276(7): 5074 - 5084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Parks, D. B. Frank, C. Huff, C. Renfrew Haft, J. Martin, X. Meng, M. P. de Caestecker, J. G. McNally, A. Reddi, S. I. Taylor, et al. Sorting Nexin 6, a Novel SNX, Interacts with the Transforming Growth Factor-beta Family of Receptor Serine-Threonine Kinases J. Biol. Chem., May 25, 2001; 276(22): 19332 - 19339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Borrelly, J.-C. Boyer, B. Touraine, W. Szponarski, M. Rambier, and R. Gibrat The yeast mutant vps5Delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity PNAS, August 14, 2001; 98(17): 9660 - 9665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Jones, S. K. Foreman, B. B. Shank, and R. C. Kurten EGF receptor downregulation depends on a trafficking motif in the distal tyrosine kinase domain Am J Physiol Cell Physiol, March 1, 2002; 282(3): C420 - C433. [Abstract] [Full Text] [PDF]
|
|
