![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
N Hui, N Nakamura, B Sonnichsen, DT Shima, T Nilsson and G Warren
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
The early Golgi t-SNARE (target-membrane-associated soluble-N- ethylmaleimide-sensitive factor attachment protein receptor) syntaxin 5 is thought to specify the docking site for both COPI and COPII coated vesicles originating from the endoplasmic reticulum (ER) and COPI vesicles on the retrograde pathway. We now show that there are two forms of syntaxin 5 that appear to be generated from the same mRNA by alternative initiation of translation. The short form (35 kDa) corresponds to the published sequence. The long form (42 kDa) has an N- terminal cytoplasmic extension containing a predicted type II ER retrieval signal. When grafted onto a reporter molecule, this signal localized the construct to the ER. Biochemical fractionation and immunofluorescence microscopy showed that there was less of the long form in the Golgi apparatus and more in peripheral punctate structures, some of which colocalized with markers of the intermediate compartment. The predicted absence of the long form in budding yeast points to a function unique to higher organisms.
This article has been cited by other articles:
|
|
 |
|
  L. Geng, W. Boehmerle, Y. Maeda, D. Y. Okuhara, X. Tian, Z. Yu, C.-u. Choe, G. I. Anyatonwu, B. E. Ehrlich, and S. Somlo Syntaxin 5 regulates the endoplasmic reticulum channel-release properties of polycystin-2 PNAS, October 14, 2008; 105(41): 15920 - 15925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Diao, L. Frost, Y. Morohashi, and M. Lowe Coordination of Golgin Tethering and SNARE Assembly: GM130 BINDS SYNTAXIN 5 IN A p115-REGULATED MANNER J. Biol. Chem., March 14, 2008; 283(11): 6957 - 6967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. T. Mai, M. Nomura, K. Takegawa, E. Asamizu, S. Sato, T. Kato, S. Tabata, and S. Tajima Identification of a Sed5-like SNARE Gene LjSYP32-1 that Contributes to Nodule Tissue Formation of Lotus japonicus Plant Cell Physiol., July 1, 2006; 47(7): 829 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sharma, S. H. Low, S. Misra, B. Pallavi, and T. Weimbs Apical targeting of syntaxin 3 is essential for epithelial cell polarity J. Cell Biol., June 19, 2006; 173(6): 937 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Appenzeller-Herzog and H.-P. Hauri The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function J. Cell Sci., June 1, 2006; 119(11): 2173 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Breuza, R. Halbeisen, P. Jeno, S. Otte, C. Barlowe, W. Hong, and H.-P. Hauri Proteomics of Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC) Membranes from Brefeldin A-treated HepG2 Cells Identifies ERGIC-32, a New Cycling Protein That Interacts with Human Erv46 J. Biol. Chem., November 5, 2004; 279(45): 47242 - 47253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Volchuk, M. Ravazzola, A. Perrelet, W. S. Eng, M. Di Liberto, O. Varlamov, M. Fukasawa, T. Engel, T. H. Sollner, J. E. Rothman, et al. Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack Mol. Biol. Cell, April 1, 2004; 15(4): 1506 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lowe, J. D. Lane, P. G. Woodman, and V. J. Allan Caspase-mediated cleavage of syntaxin 5 and giantin accompanies inhibition of secretory traffic during apoptosis J. Cell Sci., March 1, 2004; 117(7): 1139 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Woodman p97, a protein coping with multiple identities J. Cell Sci., November 1, 2003; 116(21): 4283 - 4290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uchiyama, E. Jokitalo, M. Lindman, M. Jackman, F. Kano, M. Murata, X. Zhang, and H. Kondo The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle J. Cell Biol., June 23, 2003; 161(6): 1067 - 1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uchiyama, E. Jokitalo, F. Kano, M. Murata, X. Zhang, B. Canas, R. Newman, C. Rabouille, D. Pappin, P. Freemont, et al. VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo J. Cell Biol., December 9, 2002; 159(5): 855 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Rancour, C. E. Dickey, S. Park, and S. Y. Bednarek Characterization of AtCDC48. Evidence for Multiple Membrane Fusion Mechanisms at the Plane of Cell Division in Plants Plant Physiology, November 1, 2002; 130(3): 1241 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Watson and J. E. Pessin Transmembrane domain length determines intracellular membrane compartment localization of syntaxins 3, 4, and 5 Am J Physiol Cell Physiol, July 1, 2001; 281(1): C215 - C223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kasai and K. Akagawa Roles of the cytoplasmic and transmembrane domains of syntaxins in intracellular localization and trafficking J. Cell Sci., January 9, 2001; 114(17): 3115 - 3124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Roy, J. J.M. Bergeron, C. Lavoie, R. Hendriks, J. Gushue, A. Fazel, A. Pelletier, D. J. Morré, V. N. Subramaniam, W. Hong, et al. Role of p97 and Syntaxin 5 in the Assembly of Transitional Endoplasmic Reticulum Mol. Biol. Cell, August 1, 2000; 11(8): 2529 - 2542. [Abstract] [Full Text]
|
|
|
|
|
|
K. Hatsuzawa, H. Hirose, K. Tani, A. Yamamoto, R. H. Scheller, and M. Tagaya Syntaxin 18, a SNAP Receptor That Functions in the Endoplasmic Reticulum, Intermediate Compartment, and cis-Golgi Vesicle Trafficking J. Biol. Chem., April 28, 2000; 275(18): 13713 - 13720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Watson and J. E. Pessin Functional Cooperation of Two Independent Targeting Domains in Syntaxin 6 Is Required for Its Efficient Localization in the trans-Golgi Network of 3T3L1 Adipocytes J. Biol. Chem., January 14, 2000; 275(2): 1261 - 1268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Cikaluk, N. Tahbaz, L. C. Hendricks, G. E. DiMattia, D. Hansen, D. Pilgrim, and T. C. Hobman GERp95, a Membrane-associated Protein that Belongs to a Family of Proteins Involved in Stem Cell Differentiation Mol. Biol. Cell, October 1, 1999; 10(10): 3357 - 3372. [Abstract] [Full Text]
|
|
|
|
|
|
C Rabouille, D. Kuntz, A Lockyer, R Watson, T Signorelli, D. Rose, M van den Heuvel, and D. Roberts The Drosophila GMII gene encodes a Golgi alpha-mannosidase II J. Cell Sci., January 10, 1999; 112(19): 3319 - 3330. [Abstract] [PDF]
|
|
|
|
 |
|
 C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Steegmaier, B. Yang, J.-S. Yoo, B. Huang, M. Shen, S. Yu, Y. Luo, and R. H. Scheller Three Novel Proteins of the Syntaxin/SNAP-25 Family J. Biol. Chem., December 18, 1998; 273(51): 34171 - 34179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Fernandez and G. Warren In Vitro Synthesis of Sulfated Glycosaminoglycans Coupled to Inter-compartmental Golgi Transport J. Biol. Chem., July 24, 1998; 273(30): 19030 - 19039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hay, J. Klumperman, V. Oorschot, M. Steegmaier, C. S. Kuo, and R. H. Scheller Localization, Dynamics, and Protein Interactions Reveal Distinct Roles for ER and Golgi SNAREs J. Cell Biol., June 29, 1998; 141(7): 1489 - 1502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Rowe, C. Dascher, S. Bannykh, H. Plutner, and W. E. Balch Role of Vesicle-Associated Syntaxin 5 in the Assembly of Pre-Golgi Intermediates Science, January 30, 1998; 279(5351): 696 - 700. [Abstract] [Full Text]
|
|
|
|
|
|
W Hong Protein transport from the endoplasmic reticulum to the Golgi apparatus J. Cell Sci., January 10, 1998; 111(19): 2831 - 2839. [Abstract] [PDF]
|
|
|
|
|
|
D. Xu, A. P. Joglekar, A. L. Williams, and J. C. Hay Subunit Structure of a Mammalian ER/Golgi SNARE Complex J. Biol. Chem., December 8, 2000; 275(50): 39631 - 39639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Zarei, N. Zhu, A. Alioua, M. Eghbali, E. Stefani, and L. Toro A Novel MaxiK Splice Variant Exhibits Dominant-negative Properties for Surface Expression J. Biol. Chem., May 4, 2001; 276(19): 16232 - 16239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Zhang and W. Hong Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi Transport J. Biol. Chem., July 13, 2001; 276(29): 27480 - 27487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Orci, M. Ravazzola, A. Volchuk, T. Engel, M. Gmachl, M. Amherdt, A. Perrelet, T. H. Sollner, and J. E. Rothman Anterograde flow of cargo across the Golgi stack potentially mediated via bidirectional "percolating" COPI vesicles PNAS, September 12, 2000; 97(19): 10400 - 10405. [Abstract] [Full Text] [PDF]
|
|
