![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 1, 143-160, January 1998
*Division of Cell Biology, German Cancer Research Center, D-69120
Heidelberg, Germany; and
We report the identification and molecular characterization of a
novel type of constitutive nuclear protein that is present in diverse
vertebrate species, from Xenopus laevis to human. The cDNA-deduced amino acid sequence of the Xenopus protein
defines a polypeptide of a calculated mass of 146.2 kDa and a
isoelectric point of 6.8, with a conspicuous domain enriched in the
dipeptide TP (threonine-proline) near its amino terminus.
Immunolocalization studies in cultured cells and tissues sections of
different origin revealed an exclusive nuclear localization of the
protein. The protein is diffusely distributed in the nucleoplasm but
concentrated in nuclear speckles, which represent a subnuclear
compartment enriched in small nuclear ribonucleoprotein particles and
other splicing factors, as confirmed by colocalization with certain splicing factors and Sm proteins. During mitosis, when transcription and splicing are downregulated, the protein is released from the nuclear speckles and transiently dispersed throughout the cytoplasm. Biochemical experiments have shown that the protein is recovered in a
~12S complex, and gel filtration studies confirm that the protein is
part of a large particle. Immunoprecipitation and Western blot analysis
of chromatographic fractions enriched in human U2 small nuclear
ribonucleoprotein particles of distinct sizes (12S, 15S, and 17S),
reflecting their variable association with splicing factors SF3a and
SF3b, strongly suggests that the 146-kDa protein reported here is a
constituent of the SF3b complex.
Departément de Biologie
Cellulaire, Université de Genève, CH-1211 Genève 4, Switzerland
This article has been cited by other articles:
|
|
 |
|
  Y. Robin-Lespinasse, S. Sentis, C. Kolytcheff, M.-C. Rostan, L. Corbo, and M. Le Romancer hCAF1, a new regulator of PRMT1-dependent arginine methylation J. Cell Sci., February 15, 2007; 120(4): 638 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Isono, Y. Mizutani-Koseki, T. Komori, M. S. Schmidt-Zachmann, and H. Koseki Mammalian Polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1 Genes & Dev., March 1, 2005; 19(5): 536 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nesic, G. Tanackovic, and A. Kramer A role for Cajal bodies in the final steps of U2 snRNP biogenesis J. Cell Sci., September 1, 2004; 117(19): 4423 - 4433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Eilbracht, M. Reichenzeller, M. Hergt, M. Schnolzer, H. Heid, M. Stohr, W. W. Franke, and M. S. Schmidt-Zachmann NO66, a Highly Conserved Dual Location Protein in the Nucleolus and in a Special Type of Synchronously Replicating Chromatin Mol. Biol. Cell, April 1, 2004; 15(4): 1816 - 1832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. V. Prasanth, P. A. Sacco-Bubulya, S. G. Prasanth, and D. L. Spector Sequential Entry of Components of Gene Expression Machinery into Daughter Nuclei Mol. Biol. Cell, March 1, 2003; 14(3): 1043 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hofmann, M. Schnolzer, I. Kaufmann, and W. W. Franke Symplekin, a Constitutive Protein of Karyo- and Cytoplasmic Particles Involved in mRNA Biogenesis in Xenopus laevis Oocytes Mol. Biol. Cell, May 1, 2002; 13(5): 1665 - 1676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kneissel, W. W. Franke, J. G. Gall, H. Heid, S. Reidenbach, M. Schnolzer, H. Spring, H. Zentgraf, and M. S. Schmidt-Zachmann A Novel Karyoskeletal Protein: Characterization of Protein NO145, the Major Component of Nucleolar Cortical Skeleton in Xenopus Oocytes Mol. Biol. Cell, December 1, 2001; 12(12): 3904 - 3918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Nesic and A. Kramer Domains in Human Splicing Factors SF3a60 and SF3a66 Required for Binding to SF3a120, Assembly of the 17S U2 snRNP, and Prespliceosome Formation Mol. Cell. Biol., October 1, 2001; 21(19): 6406 - 6417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Eilbracht and M. S. Schmidt-Zachmann Identification of a sequence element directing a protein to nuclear speckles PNAS, March 27, 2001; 98(7): 3849 - 3854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Zirwes, J. Eilbracht, S. Kneissel, and M. S. Schmidt-Zachmann A Novel Helicase-Type Protein in the Nucleolus: Protein NOH61 Mol. Biol. Cell, April 1, 2000; 11(4): 1153 - 1167. [Abstract] [Full Text]
|
|
|
|
|
|
M. H. Pauling, D. S. McPheeters, and M. Ares Jr. Functional Cus1p Is Found with Hsh155p in a Multiprotein Splicing Factor Associated with U2 snRNA Mol. Cell. Biol., March 15, 2000; 20(6): 2176 - 2185. [Abstract] [Full Text]
|
|
|
|
|
|
R. Perriman and M. Ares Jr. ATP can be dispensable for prespliceosome formation in yeast Genes & Dev., January 1, 2000; 14(1): 97 - 107. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. R. Ladomery, J. Slight, S. Mc Ghee, and N. D. Hastie Presence of WT1, the Wilm's Tumor Suppressor Gene Product, in Nuclear Poly(A)+ Ribonucleoprotein J. Biol. Chem., December 17, 1999; 274(51): 36520 - 36526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Yan, R. Perriman, H. Igel, K. J. Howe, M. Neville, and M. Ares Jr. CUS2, a Yeast Homolog of Human Tat-SF1, Rescues Function of Misfolded U2 through an Unusual RNA Recognition Motif Mol. Cell. Biol., September 1, 1998; 18(9): 5000 - 5009. [Abstract] [Full Text]
|
|
|
|
|
|
W. Seghezzi, K. Chua, F. Shanahan, O. Gozani, R. Reed, and E. Lees Cyclin E Associates with Components of the Pre-mRNA Splicing Machinery in Mammalian Cells Mol. Cell. Biol., August 1, 1998; 18(8): 4526 - 4536. [Abstract] [Full Text]
|
|
|
|
|
|
P Tate, M Lee, S Tweedie, W. Skarnes, and W. Bickmore Capturing novel mouse genes encoding chromosomal and other nuclear proteins J. Cell Sci., January 9, 1998; 111(17): 2575 - 2585. [Abstract] [PDF]
|
|
