![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
and
*Institut Jacques Monod, Centre National de la Recherche
Scientifique-UMRC7592, Université Paris 7-Denis Diderot,
Paris Cedex 05, France;
The ORF YOL018c (TLG2) of Saccharomyces
cerevisiae encodes a protein that belongs to the syntaxin
protein family. The proteins of this family, t-SNAREs, are
present on target organelles and are thought to participate in the
specific interaction between vesicles and acceptor membranes in
intracellular membrane trafficking. TLG2 is not an
essential gene, and its deletion does not cause defects in the
secretory pathway. However, its deletion in cells lacking the vacuolar
ATPase subunit Vma2p leads to loss of viability, suggesting that Tlg2p
is involved in endocytosis. In tlg2VTT Biotechnology and Food
Research, FIN-02044 VTT, Finland;
Biozentrum, University
of Basel, CH-4056 Basel, Switzerland; and
§Department of
Molecular Genetics and Cell Biology, The University of Chicago,
Chicago, Illinois 60637
cells, internalization was normal for two endocytic markers, the pheromone 
-factor and the plasma membrane uracil permease. In contrast, degradation of -factor and uracil permease was delayed in
tlg2 cells. Internalization of positively charged
Nanogold shows that the endocytic pathway is perturbed in the mutant,
which accumulates Nanogold in primary endocytic vesicles and shows a
greatly reduced complement of early endosomes. These results strongly
suggest that Tlg2p is a t-SNARE involved in early endosome biogenesis.
Corresponding author. E-mail
address: haguenauer{at}ijm.jussieu.fr.
This article has been cited by other articles:
|
|
 |
|
  J.-O. De Craene, J. Coleman, P. Estrada de Martin, M. Pypaert, S. Anderson, J. R. Yates III, S. Ferro-Novick, and P. Novick Rtn1p Is Involved in Structuring the Cortical Endoplasmic Reticulum Mol. Biol. Cell, July 1, 2006; 17(7): 3009 - 3020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. N. Carpp, L. F. Ciufo, S. G. Shanks, A. Boyd, and N. J. Bryant The Sec1p/Munc18 protein Vps45p binds its cognate SNARE proteins via two distinct modes J. Cell Biol., June 19, 2006; 173(6): 927 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Robinson, P. P. Poon, C. Schindler, L. E. Murray, R. Kama, G. Gabriely, R. A. Singer, A. Spang, G. C. Johnston, and J. E. Gerst The Gcs1 Arf-GAP Mediates Snc1,2 v-SNARE Retrieval to the Golgi in Yeast Mol. Biol. Cell, April 1, 2006; 17(4): 1845 - 1858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Geng, M. E. Shin, P. M. Gilbert, R. N. Collins, and C. G. Burd Saccharomyces cerevisiae Rab-GDI Displacement Factor Ortholog Yip3p Forms Distinct Complexes with the Ypt1 Rab GTPase and the Reticulon Rtn1p Eukaryot. Cell, July 1, 2005; 4(7): 1166 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Reggiori, C.-W. Wang, U. Nair, T. Shintani, H. Abeliovich, and D. J. Klionsky Early Stages of the Secretory Pathway, but Not Endosomes, Are Required for Cvt Vesicle and Autophagosome Assembly in Saccharomyces cerevisiae Mol. Biol. Cell, May 1, 2004; 15(5): 2189 - 2204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Calero, C. Z. Chen, W. Zhu, N. Winand, K. A. Havas, P. M. Gilbert, C. G. Burd, and R. N. Collins Dual Prenylation Is Required for Rab Protein Localization and Function Mol. Biol. Cell, May 1, 2003; 14(5): 1852 - 1867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Misu, K. Fujimura-Kamada, T. Ueda, A. Nakano, H. Katoh, and K. Tanaka Cdc50p, a Conserved Endosomal Membrane Protein, Controls Polarized Growth in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2003; 14(2): 730 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Luo and D. Gallwitz Biochemical and Genetic Evidence for the Involvement of Yeast Ypt6-GTPase in Protein Retrieval to Different Golgi Compartments J. Biol. Chem., January 3, 2003; 278(2): 791 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gurunathan, M. Marash, A. Weinberger, and J. E. Gerst t-SNARE Phosphorylation Regulates Endocytosis in Yeast Mol. Biol. Cell, May 1, 2002; 13(5): 1594 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Belgareh-Touze, S. Avaro, Y. Rouille, B. Hoflack, and R. Haguenauer-Tsapis Yeast Vps55p, a Functional Homolog of Human Obesity Receptor Gene-related Protein, Is Involved in Late Endosome to Vacuole Trafficking Mol. Biol. Cell, May 1, 2002; 13(5): 1694 - 1708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform J. Cell Biol., February 18, 2002; 156(4): 653 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Paumet, B. Brugger, F. Parlati, J. A. McNew, T. H. Sollner, and J. E. Rothman A t-SNARE of the endocytic pathway must be activated for fusion J. Cell Biol., December 10, 2001; 155(6): 961 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Brickner, J. M. Blanchette, G. Sipos, and R. S. Fuller The Tlg SNARE complex is required for TGN homotypic fusion J. Cell Biol., December 10, 2001; 155(6): 969 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Abeliovich and D. J. Klionsky Autophagy in Yeast: Mechanistic Insights and Physiological Function Microbiol. Mol. Biol. Rev., September 1, 2001; 65(3): 463 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Galan, A. Wiederkehr, J. H. Seol, R. Haguenauer-Tsapis, R. J. Deshaies, H. Riezman, and M. Peter Skp1p and the F-Box Protein Rcy1p Form a Non-SCF Complex Involved in Recycling of the SNARE Snc1p in Yeast Mol. Cell. Biol., May 1, 2001; 21(9): 3105 - 3117. [Abstract] [Full Text]
|
|
|
|
|
|
A. Spang, J. M. Herrmann, S. Hamamoto, and R. Schekman The ADP Ribosylation Factor-Nucleotide Exchange Factors Gea1p and Gea2p Have Overlapping, but Not Redundant Functions in Retrograde Transport from the Golgi to the Endoplasmic Reticulum Mol. Biol. Cell, April 1, 2001; 12(4): 1035 - 1045. [Abstract] [Full Text]
|
|
|
|
|
|
O. W. Rossanese, C. A. Reinke, B. J. Bevis, A. T. Hammond, I. B. Sears, J. O'Connor, and B. S. Glick A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae J. Cell Biol., March 26, 2001; 153(1): 47 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Sanderfoot, M. Pilgrim, L. Adam, and N. V. Raikhel Disruption of Individual Members of Arabidopsis Syntaxin Gene Families Indicates Each Has Essential Functions PLANT CELL, March 1, 2001; 13(3): 659 - 666. [Abstract] [Full Text]
|
|
|
|
|
|
E. Grote, G. Vlacich, M. Pypaert, and P. J. Novick A snc1 Endocytosis Mutant: Phenotypic Analysis and Suppression by Overproduction of Dihydrosphingosine Phosphate Lyase Mol. Biol. Cell, December 1, 2000; 11(12): 4051 - 4065. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. A. Sanderfoot, F. F. Assaad, and N. V. Raikhel The Arabidopsis Genome. An Abundance of Soluble N-Ethylmaleimide-Sensitive Factor Adaptor Protein Receptors Plant Physiology, December 1, 2000; 124(4): 1558 - 1569. [Abstract] [Full Text]
|
|
|
|
|
|
S. Gurunathan, D. Chapman-Shimshoni, S. Trajkovic, and J. E. Gerst Yeast Exocytic v-SNAREs Confer Endocytosis Mol. Biol. Cell, October 1, 2000; 11(10): 3629 - 3643. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Bassham, A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel AtVPS45 Complex Formation at the trans-Golgi Network Mol. Biol. Cell, July 1, 2000; 11(7): 2251 - 2265. [Abstract] [Full Text]
|
|
|
|
|
|
T. P. Levine, C. A.R. Wiggins, and S. Munro Inositol Phosphorylceramide Synthase Is Located in the Golgi Apparatus of Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2000; 11(7): 2267 - 2281. [Abstract] [Full Text]
|
|
|
|
|
|
A. Wiederkehr, S. Avaro, C. Prescianotto-Baschong, R. Haguenauer-Tsapis, and H. Riezman The F-box Protein Rcy1p Is Involved in Endocytic Membrane Traffic and Recycling Out of an Early Endosome in Saccharomyces cerevisiae J. Cell Biol., April 17, 2000; 149(2): 397 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Panek, E Conibear, J. Bryan, R. Colvin, C. Goshorn, and L. Robinson Identification of Rgp1p, a novel Golgi recycling factor, as a protein required for efficient localization of yeast casein kinase 1 to the plasma membrane J. Cell Sci., January 12, 2000; 113(24): 4545 - 4555. [Abstract] [PDF]
|
|
|
|
|
|
B Gagny, A Wiederkehr, P Dumoulin, B Winsor, H Riezman, and R Haguenauer-Tsapis A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis J. Cell Sci., January 9, 2000; 113(18): 3309 - 3319. [Abstract] [PDF]
|
|
|
|
|
|
M. J. Lewis, B. J. Nichols, C. Prescianotto-Baschong, H. Riezman, and H. R. B. Pelham Specific Retrieval of the Exocytic SNARE Snc1p from Early Yeast Endosomes Mol. Biol. Cell, January 1, 2000; 11(1): 23 - 38. [Abstract] [Full Text]
|
|
|
|
|
|
E. Conibear and T. H. Stevens Vps52p, Vps53p, and Vps54p Form a Novel Multisubunit Complex Required for Protein Sorting at the Yeast Late Golgi Mol. Biol. Cell, January 1, 2000; 11(1): 305 - 323. [Abstract] [Full Text]
|
|
|
|
|
|
A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel The t-SNARE AtVAM3p Resides on the Prevacuolar Compartment in Arabidopsis Root Cells Plant Physiology, November 1, 1999; 121(3): 929 - 938. [Abstract] [Full Text]
|
|
|
|
|
|
J. G. S. Coe, A. C. B. Lim, J. Xu, and W. Hong A Role for Tlg1p in the Transport of Proteins within the Golgi Apparatus of Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 1999; 10(7): 2407 - 2423. [Abstract] [Full Text]
|
|
|
|
|
|
G. F. von Mollard and T. H. Stevens The Saccharomyces cerevisiae v-SNARE Vti1p Is Required for Multiple Membrane Transport Pathways to the Vacuole Mol. Biol. Cell, June 1, 1999; 10(6): 1719 - 1732. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Peng, R. Grabowski, A. De Antoni, and D. Gallwitz Specific interaction of the yeast cis-Golgi syntaxin Sed5p and the coat protein complex II component Sec24p of endoplasmic reticulum-derived transport vesicles PNAS, March 30, 1999; 96(7): 3751 - 3756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bogdanovic, F. Bruckert, T. Morio, and M. Satre A Syntaxin 7 Homologue Is Present in Dictyostelium discoideum Endosomes and Controls Their Homotypic Fusion J. Biol. Chem., November 17, 2000; 275(47): 36691 - 36697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform J. Cell Biol., February 18, 2002; 156(4): 653 - 664. [Abstract] [Full Text] [PDF]
|
|
