![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Division of Biology and Medicine, Brown University, Providence,
Rhode Island 02912
The Nucleolar Localization Elements (NoLEs) of Xenopus
laevis U3 small nucleolar RNA (snoRNA) have been defined.
Fluorescein-labeled wild-type U3 snoRNA injected into
Xenopus oocyte nuclei localized specifically to nucleoli
as shown by fluorescence microscopy. Injection of mutated U3 snoRNA
revealed that the 5' region containing Boxes A and A', known to
be important for rRNA processing, is not essential for nucleolar
localization. Nucleolar localization of U3 snoRNA was independent of
the presence and nature of the 5' cap and the terminal stem. In
contrast, Boxes C and D, common to the Box C/D snoRNA family, are
critical elements for U3 localization. Mutation of the hinge region,
Box B, or Box C' led to reduced U3 nucleolar localization. Results of
competition experiments suggested that Boxes C and D act in a
cooperative manner. It is proposed that Box B facilitates U3 snoRNA
nucleolar localization by the primary NoLEs (Boxes C and D), with the
hinge region of U3 subsequently base pairing to the external
transcribed spacer of pre-rRNA, thus positioning U3 snoRNA for its
roles in rRNA processing.
This article has been cited by other articles:
|
|
 |
|
  H. Qiu, J. Eifert, L. Wacheul, M. Thiry, A. C. Berger, J. Jakovljevic, J. L. Woolford Jr., A. H. Corbett, D. L. J. Lafontaine, R. M. Terns, et al. Identification of Genes That Function in the Biogenesis and Localization of Small Nucleolar RNAs in Saccharomyces cerevisiae Mol. Cell. Biol., June 1, 2008; 28(11): 3686 - 3699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Borovjagin and S. A. Gerbi An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA Nucleic Acids Res., September 6, 2005; 33(15): 4995 - 5005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Granneman, J. Vogelzangs, R. Luhrmann, W. J. van Venrooij, G. J. M. Pruijn, and N. J. Watkins Role of Pre-rRNA Base Pairing and 80S Complex Formation in Subnucleolar Localization of the U3 snoRNP Mol. Cell. Biol., October 1, 2004; 24(19): 8600 - 8610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. BOROVJAGIN and S. A. GERBI Xenopus U3 snoRNA docks on pre-rRNA through a novel base-pairing interaction RNA, June 1, 2004; 10(6): 942 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. MARMIER-GOURRIER, A. CLERY, V. SENTY-SEGAULT, B. CHARPENTIER, F. SCHLOTTER, F. LECLERC, R. FOURNIER, and C. BRANLANT A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA RNA, July 1, 2003; 9(7): 821 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Watkins, A. Dickmanns, and R. Luhrmann Conserved Stem II of the Box C/D Motif Is Essential for Nucleolar Localization and Is Required, Along with the 15.5K Protein, for the Hierarchical Assembly of the Box C/D snoRNP Mol. Cell. Biol., December 1, 2002; 22(23): 8342 - 8352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dez, J. Noaillac-Depeyre, M. Caizergues-Ferrer, and Y. Henry Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs Mol. Cell. Biol., October 15, 2002; 22(20): 7053 - 7065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Gerbi and T. S. Lange All Small Nuclear RNAs (snRNAs) of the [U4/U6.U5] Tri-snRNP Localize to Nucleoli; Identification of the Nucleolar Localization Element of U6 snRNA Mol. Biol. Cell, September 1, 2002; 13(9): 3123 - 3137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. King, W. A. Decatur, E. Bertrand, E. S. Maxwell, and M. J. Fournier A Well-Connected and Conserved Nucleoplasmic Helicase Is Required for Production of Box C/D and H/ACA snoRNAs and Localization of snoRNP Proteins Mol. Cell. Biol., November 15, 2001; 21(22): 7731 - 7746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Borovjagin and S. A. Gerbi Xenopus U3 snoRNA GAC-Box A' and Box A Sequences Play Distinct Functional Roles in rRNA Processing Mol. Cell. Biol., September 15, 2001; 21(18): 6210 - 6221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pederson Fluorescent RNA cytochemistry: tracking gene transcripts in living cells Nucleic Acids Res., March 1, 2001; 29(5): 1013 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.A. GERBI, A.V. BOROVJAGIN, M. EZROKHI, and T.S. LANGE Ribosome Biogenesis: Role of Small Nucleolar RNA in Maturation of Eukaryotic rRNA Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 575 - 590. [Abstract] [PDF]
|
|
|
|
|
|
M. N. Schnare, J. C. Collings, D. F. Spencer, and M. W. Gray The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor Nucleic Acids Res., September 15, 2000; 28(18): 3452 - 3461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Lukowiak, S. Granneman, S. A. Mattox, W. A. Speckmann, K. Jones, H. Pluk, W. J. v. Venrooij, R. M. Terns, and M. P. Terns Interaction of the U3-55k protein with U3 snoRNA is mediated by the Box B/C motif of U3 and the WD repeats of U3-55k Nucleic Acids Res., September 15, 2000; 28(18): 3462 - 3471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Lange and S. A. Gerbi Transient Nucleolar Localization Of U6 Small Nuclear RNA In Xenopus Laevis Oocytes Mol. Biol. Cell, July 1, 2000; 11(7): 2419 - 2428. [Abstract] [Full Text]
|
|
|
|
|
|
D. G. Eickbush, D. D. Luan, and T. H. Eickbush Integration of Bombyx mori R2 Sequences into the 28S Ribosomal RNA Genes of Drosophila melanogaster Mol. Cell. Biol., January 1, 2000; 20(1): 213 - 223. [Abstract] [Full Text]
|
|
|
|
|
|
W. Speckmann, A. Narayanan, R. Terns, and M. P. Terns Nuclear Retention Elements of U3 Small Nucleolar RNA Mol. Cell. Biol., December 1, 1999; 19(12): 8412 - 8421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. PEDERSON Movement and localization of RNA in the cell nucleus FASEB J, December 1, 1999; 13(9002): 238S - 242S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Gall, M. Bellini, Z.'a. Wu, and C. Murphy Assembly of the Nuclear Transcription and Processing Machinery: Cajal Bodies (Coiled Bodies) and Transcriptosomes Mol. Biol. Cell, December 1, 1999; 10(12): 4385 - 4402. [Abstract] [Full Text]
|
|
|
|
|
|
T. S. Lange, M. Ezrokhi, F. Amaldi, and S. A. Gerbi Box H and Box ACA Are Nucleolar Localization Elements of U17 Small Nucleolar RNA Mol. Biol. Cell, November 1, 1999; 10(11): 3877 - 3890. [Abstract] [Full Text]
|
|
|
|
|
|
A. Narayanan, W. Speckmann, R. Terns, and M. P. Terns Role of the Box C/D Motif in Localization of Small Nucleolar RNAs to Coiled Bodies and Nucleoli Mol. Biol. Cell, July 1, 1999; 10(7): 2131 - 2147. [Abstract] [Full Text]
|
|
