![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 11, 3057-3069, November 1998
Programme in Cell Biology, The Hospital for Sick Children, Toronto,
Ontario, Canada M5G 1X8
Insulin can regulate the abundance and organization of filamentous
actin within cells in culture. Early studies using cell lines that
overexpress the insulin receptor demonstrated that insulin caused a
rapid reversible disassembly of actin filaments that coincided with the
rapid tyrosine dephosphorylation of focal adhesion kinase. We have
extended these studies by demonstrating that paxillin, another focal
adhesion protein, and Src undergo tyrosine dephosphorylation in
response to insulin in Chinese hamster ovary (CHO) and rat hepatoma
(HTC) cells that overexpress the insulin receptor. This contrasted with
the effect of insulin in parental CHO and HTC cells in which focal
adhesion proteins were not dephosphorylated in response to the hormone.
In addition, insulin caused a dispersion of focal adhesion proteins and
disruption of actin filament bundles only in cells that overexpressed
the insulin receptor. Moreover, in 3T3-L1 adipocytes, which are
considered prototypic insulin-responsive cells, actin filament assembly
was stimulated, and focal adhesion protein tyrosine phosphorylation was
not altered. 3T3-L1 cells have more insulin receptors than either
parental CHO or HTC cells but have fivefold less insulin receptors than
the overexpressing cell lines. We hypothesize that a threshold may
exist in which the overexpression of insulin receptors determines how
insulin signaling pathways regulate the actin cytoskeleton.
This article has been cited by other articles:
|
|
 |
|
  G. N. Hagan, Y. Lin, M. A. Magnuson, J. Avruch, and M. P. Czech A Rictor-Myo1c Complex Participates in Dynamic Cortical Actin Events in 3T3-L1 Adipocytes Mol. Cell. Biol., July 1, 2008; 28(13): 4215 - 4226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kruger, I. Kratchmarova, B. Blagoev, Y.-H. Tseng, C. R. Kahn, and M. Mann Dissection of the insulin signaling pathway via quantitative phosphoproteomics PNAS, February 19, 2008; 105(7): 2451 - 2456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L'Abbate, D. Neglia, C. Vecoli, M. Novelli, V. Ottaviano, S. Baldi, R. Barsacchi, A. Paolicchi, P. Masiello, G. S. Drummond, et al. Beneficial effect of heme oxygenase-1 expression on myocardial ischemia-reperfusion involves an increase in adiponectin in mildly diabetic rats Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3532 - H3541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Frasca, G. Pandini, R. Malaguarnera, A. Mandarino, R. L. Messina, L. Sciacca, A. Belfiore, and R. Vigneri Role of c-Abl in Directing Metabolic versus Mitogenic Effects in Insulin Receptor Signaling J. Biol. Chem., September 7, 2007; 282(36): 26077 - 26088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S.L. Thong, P. J. Bilan, and A. Klip The Rab GTPase-Activating Protein AS160 Integrates Akt, Protein Kinase C, and AMP-Activated Protein Kinase Signals Regulating GLUT4 Traffic Diabetes, February 1, 2007; 56(2): 414 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sbrissa, O. C. Ikonomov, J. Strakova, and A. Shisheva Role for a Novel Signaling Intermediate, Phosphatidylinositol 5-Phosphate, in Insulin-Regulated F-Actin Stress Fiber Breakdown and GLUT4 Translocation Endocrinology, November 1, 2004; 145(11): 4853 - 4865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ibarrola, H. Molina, A. Iwahori, and A. Pandey A Novel Proteomic Approach for Specific Identification of Tyrosine Kinase Substrates Using [13C]Tyrosine J. Biol. Chem., April 16, 2004; 279(16): 15805 - 15813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Huang, A. T. Cheung, J. T. Parsons, and M. Bryer-Ash Focal Adhesion Kinase (FAK) Regulates Insulin-stimulated Glycogen Synthesis in Hepatocytes J. Biol. Chem., May 10, 2002; 277(20): 18151 - 18160. [Abstract] [Full Text] [PDF]
|
|
