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Vol. 9, Issue 11, 3211-3225, November 1998
and
*CREST Research Project, Department of Biophysics, Graduate
School of Science, Kyoto University, Kyoto 606, Japan;
In higher eukaryotic cells, the spindle forms along with chromosome
condensation in mitotic prophase. In metaphase, chromosomes are aligned
on the spindle with sister kinetochores facing toward the
opposite poles. In anaphase A, sister chromatids separate from each
other without spindle extension, whereas spindle elongation takes place
during anaphase B. We have critically examined whether such mitotic
stages also occur in a lower eukaryote, Schizosaccharomyces pombe. Using the green fluorescent protein tagging technique, early mitotic to late anaphase events were observed in living fission
yeast cells. S. pombe has three phases in spindle
dynamics, spindle formation (phase 1), constant spindle length (phase
2), and spindle extension (phase 3). Sister centromere separation (anaphase A) rapidly occurred at the end of phase 2. The centromere showed dynamic movements throughout phase 2 as it moved back and forth
and was transiently split in two before its separation, suggesting that
the centromere was positioned in a bioriented manner toward the poles
at metaphase. Microtubule-associating Dis1 was required for the
occurrence of constant spindle length and centromere movement in phase
2. Normal transition from phase 2 to 3 needed DNA topoisomerase II and
Cut1 but not Cut14. The duration of each phase was highly dependent on temperature.
Department of Physiology, University of California San
Francisco, San Francisco, California 94143-0448; and
Kansai Advanced Research Center, Communications Research
Laboratory, Kobe 651-24, Japan
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