![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 12, 3383-3397, December 1998
Medical Research Council Laboratory of Molecular Biology,
Cambridge CB2 2QH, United Kingdom
Tlg1p and Tlg2p, members of the syntaxin family of
SNAREs in yeast, have been implicated in both endocytosis and
the retention of late Golgi markers. We have investigated the functions
of these and the other endocytic syntaxins Pep12p and Vam3p.
Remarkably, growth is possible in the absence of all four proteins. In
the absence of the others, Pep12p and Tlg1p can each create endosomes accessible to the endocytic tracer dye FM4-64. However, although Pep12p is required for the ligand-induced internalization of the 
factor receptor and its passage via Pep12p-containing membranes to the
vacuole, Tlg1p is not. In contrast, Tlg1p is required for the efficient
localization of the catalytic subunit of chitin synthase III
(Chs3p) to the bud neck, a process that involves endocytosis and
polarized delivery of Chs3p. In wild-type cells, internalized Chs3p
cofractionates with Tlg1p and Tlg2p, and in a strain lacking the other
endocytic syntaxins, either Tlg1p or Tlg2p is sufficient for correct
localization of the enzyme. Pep12p is neither necessary nor sufficient
for this process. We conclude that there are two endocytic routes in
yeast that can operate independently and that Tlg1p is located at the
junction of one of these with the polarized exocytic pathway.
Corresponding author. E-mail address:
hp{at}mrc-lmb.cam.ac.uk.
This article has been cited by other articles:
|
|
 |
|
  C. J. Mousley, K. Tyeryar, K. E. Ile, G. Schaaf, R. L. Brost, C. Boone, X. Guan, M. R. Wenk, and V. A. Bankaitis Trans-Golgi Network and Endosome Dynamics Connect Ceramide Homeostasis with Regulation of the Unfolded Protein Response and TOR Signaling in Yeast Mol. Biol. Cell, November 1, 2008; 19(11): 4785 - 4803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sanchatjate and R. Schekman Chs5/6 Complex: A Multiprotein Complex That Interacts with and Conveys Chitin Synthase III from the Trans-Golgi Network to the Cell Surface Mol. Biol. Cell, October 1, 2006; 17(10): 4157 - 4166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. T. Mai, M. Nomura, K. Takegawa, E. Asamizu, S. Sato, T. Kato, S. Tabata, and S. Tajima Identification of a Sed5-like SNARE Gene LjSYP32-1 that Contributes to Nodule Tissue Formation of Lotus japonicus Plant Cell Physiol., July 1, 2006; 47(7): 829 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Huckaba, A. C. Gay, L. F. Pantalena, H.-C. Yang, and L. A. Pon Live cell imaging of the assembly, disassembly, and actin cable-dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae J. Cell Biol., November 8, 2004; 167(3): 519 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wicky, H. Schwarz, and B. Singer-Kruger Molecular Interactions of Yeast Neo1p, an Essential Member of the Drs2 Family of Aminophospholipid Translocases, and Its Role in Membrane Trafficking within the Endomembrane System Mol. Cell. Biol., September 1, 2004; 24(17): 7402 - 7418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Beh and J. Rine A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution J. Cell Sci., June 15, 2004; 117(14): 2983 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lafourcade, J.-M. Galan, Y. Gloor, R. Haguenauer-Tsapis, and M. Peter The GTPase-Activating Enzyme Gyp1p Is Required for Recycling of Internalized Membrane Material by Inactivation of the Rab/Ypt GTPase Ypt1p Mol. Cell. Biol., May 1, 2004; 24(9): 3815 - 3826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Lisman, T. Pomorski, C. Vogelzangs, D. Urli-Stam, W. de Cocq van Delwijnen, and J. C. M. Holthuis Protein Sorting in the Late Golgi of Saccharomyces cerevisiae Does Not Require Mannosylated Sphingolipids J. Biol. Chem., January 9, 2004; 279(2): 1020 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nikko, A.-M. Marini, and B. Andre Permease Recycling and Ubiquitination Status Reveal a Particular Role for Bro1 in the Multivesicular Body Pathway J. Biol. Chem., December 12, 2003; 278(50): 50732 - 50743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Bulik, M. Olczak, H. A. Lucero, B. C. Osmond, P. W. Robbins, and C. A. Specht Chitin Synthesis in Saccharomyces cerevisiae in Response to Supplementation of Growth Medium with Glucosamine and Cell Wall Stress Eukaryot. Cell, October 1, 2003; 2(5): 886 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lafourcade, J.-M. Galan, and M. Peter Opposite Roles of the F-Box Protein Rcy1p and the GTPase-Activating Protein Gyp2p During Recycling of Internalized Proteins in Yeast Genetics, June 1, 2003; 164(2): 469 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Moore, Y. Kim, and C. C. Philpott The mechanism of ferrichrome transport through Arn1p and its metabolism in Saccharomyces cerevisiae PNAS, May 13, 2003; 100(10): 5664 - 5669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Deloche and R. W. Schekman Vps10p Cycles between the TGN and the Late Endosome via the Plasma Membrane in Clathrin Mutants Mol. Biol. Cell, December 1, 2002; 13(12): 4296 - 4307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gurunathan, M. Marash, A. Weinberger, and J. E. Gerst t-SNARE Phosphorylation Regulates Endocytosis in Yeast Mol. Biol. Cell, May 1, 2002; 13(5): 1594 - 1607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Paumet, B. Brugger, F. Parlati, J. A. McNew, T. H. Sollner, and J. E. Rothman A t-SNARE of the endocytic pathway must be activated for fusion J. Cell Biol., December 10, 2001; 155(6): 961 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Sanderfoot, V. Kovaleva, D. C. Bassham, and N. V. Raikhel Interactions between Syntaxins Identify at Least Five SNARE Complexes within the Golgi/Prevacuolar System of the Arabidopsis Cell Mol. Biol. Cell, December 1, 2001; 12(12): 3733 - 3743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Galan, A. Wiederkehr, J. H. Seol, R. Haguenauer-Tsapis, R. J. Deshaies, H. Riezman, and M. Peter Skp1p and the F-Box Protein Rcy1p Form a Non-SCF Complex Involved in Recycling of the SNARE Snc1p in Yeast Mol. Cell. Biol., May 1, 2001; 21(9): 3105 - 3117. [Abstract] [Full Text]
|
|
|
|
|
|
M. Black and H. Pelham A Selective Transport Route from Golgi to Late Endosomes that Requires the Yeast GGA Proteins J. Cell Biol., October 30, 2000; 151(3): 587 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gurunathan, D. Chapman-Shimshoni, S. Trajkovic, and J. E. Gerst Yeast Exocytic v-SNAREs Confer Endocytosis Mol. Biol. Cell, October 1, 2000; 11(10): 3629 - 3643. [Abstract] [Full Text]
|
|
|
|
|
|
A. Wiederkehr, S. Avaro, C. Prescianotto-Baschong, R. Haguenauer-Tsapis, and H. Riezman The F-box Protein Rcy1p Is Involved in Endocytic Membrane Traffic and Recycling Out of an Early Endosome in Saccharomyces cerevisiae J. Cell Biol., April 17, 2000; 149(2): 397 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Noda, J. Kim, W.-P. Huang, M. Baba, C. Tokunaga, Y. Ohsumi, and D. J. Klionsky Apg9p/Cvt7p Is an Integral Membrane Protein Required for Transport Vesicle Formation in the Cvt and Autophagy Pathways J. Cell Biol., February 7, 2000; 148(3): 465 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ono, T. Yabe, M. Sudoh, T. Nakajima, T. Yamada-Okabe, M. Arisawa, and H. Yamada-Okabe The yeast Chs4 protein stimulates the trypsin-sensitive activity of chitin synthase 3 through an apparent protein-protein interaction Microbiology, February 1, 2000; 146(2): 385 - 391. [Abstract] [Full Text]
|
|
|
|
|
|
B. Santos and M. Snyder Sbe2p and Sbe22p, Two Homologous Golgi Proteins Involved in Yeast Cell Wall Formation Mol. Biol. Cell, February 1, 2000; 11(2): 435 - 452. [Abstract] [Full Text]
|
|
|
|
|
|
H. Panek, E Conibear, J. Bryan, R. Colvin, C. Goshorn, and L. Robinson Identification of Rgp1p, a novel Golgi recycling factor, as a protein required for efficient localization of yeast casein kinase 1 to the plasma membrane J. Cell Sci., January 12, 2000; 113(24): 4545 - 4555. [Abstract] [PDF]
|
|
|
|
|
|
D Pruyne and A Bretscher Polarization of cell growth in yeast J. Cell Sci., January 2, 2000; 113(4): 571 - 585. [Abstract] [PDF]
|
|
|
|
|
|
M. J. Lewis, B. J. Nichols, C. Prescianotto-Baschong, H. Riezman, and H. R. B. Pelham Specific Retrieval of the Exocytic SNARE Snc1p from Early Yeast Endosomes Mol. Biol. Cell, January 1, 2000; 11(1): 23 - 38. [Abstract] [Full Text]
|
|
|
|
|
|
E. Conibear and T. H. Stevens Vps52p, Vps53p, and Vps54p Form a Novel Multisubunit Complex Required for Protein Sorting at the Yeast Late Golgi Mol. Biol. Cell, January 1, 2000; 11(1): 305 - 323. [Abstract] [Full Text]
|
|
|
|
|
|
E. Grote and P. J. Novick Promiscuity in Rab-SNARE Interactions Mol. Biol. Cell, December 1, 1999; 10(12): 4149 - 4161. [Abstract] [Full Text]
|
|
|
|
|
|
R. Prekeris, B. Yang, V. Oorschot, J. Klumperman, and R. H. Scheller Differential Roles of Syntaxin 7 and Syntaxin 8 in Endosomal Trafficking Mol. Biol. Cell, November 1, 1999; 10(11): 3891 - 3908. [Abstract] [Full Text]
|
|
|
|
|
|
B. C. Osmond, C. A. Specht, and P. W. Robbins Chitin synthase III: Synthetic lethal mutants and "stress related" chitin synthesis that bypasses the CSD3/CHS6 localization pathway PNAS, September 28, 1999; 96(20): 11206 - 11210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. B. Allan and W. E. Balch Protein Sorting by Directed Maturation of Golgi Compartments Science, July 2, 1999; 285(5424): 63 - 66. [Abstract] [Full Text]
|
|
|
|
|
|
G. F. von Mollard and T. H. Stevens The Saccharomyces cerevisiae v-SNARE Vti1p Is Required for Multiple Membrane Transport Pathways to the Vacuole Mol. Biol. Cell, June 1, 1999; 10(6): 1719 - 1732. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. A. Sanderfoot and N. V. Raikhel The Specificity of Vesicle Trafficking: Coat Proteins and SNAREs PLANT CELL, April 1, 1999; 11(4): 629 - 642. [Full Text]
|
|
|
|
|
|
E. Harsay and R. Schekman A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway J. Cell Biol., January 21, 2002; 156(2): 271 - 286. [Abstract] [Full Text] [PDF]
|
|
