![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 12, 3445-3453, December 1998
*Department of Pathology, Hoag Memorial Hospital Presbyterian,
Newport Beach, California 92663; and
Stearoyl-coenzyme A desaturase (SCD) is a key regulator of membrane
fluidity, turns over rapidly, and represents a prototype for selective
degradation of resident proteins of the endoplasmic reticulum. Using
detergent-solubilized, desaturase-induced rat liver microsomes we have
characterized a protease that degrades SCD. Degradation of SCD in vitro
is highly selective, has a half-life of 3-4 h, and generates a 20-kDa
C-terminal fragment of SCD. The N terminus of the 20-kDa fragment was
identified as Phe177. The cleavage site occurs in a
conserved 12-residue hydrophobic segment of SCD flanked by clusters of
basic residues. The SCD protease remains associated with microsomal
membranes after peripheral and lumenal proteins have been selectively
removed. SCD protease is present in normal rat liver microsomes
and cleaves purified SCD. We conclude that rapid turnover of SCD
involves a constitutive microsomal protease with properties of an
integral membrane protein.
Department of Biochemistry,
University of Connecticut Health Center, Farmington, Connecticut
06030-3305
Corresponding author.
This article has been cited by other articles:
|
|
 |
|
  U. R. Mbonye, C. Yuan, C. E. Harris, R. S. Sidhu, I. Song, T. Arakawa, and W. L. Smith Two Distinct Pathways for Cyclooxygenase-2 Protein Degradation J. Biol. Chem., March 28, 2008; 283(13): 8611 - 8623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Drosatos, D. Sanoudou, K. E. Kypreos, D. Kardassis, and V. I. Zannis A Dominant Negative Form of the Transcription Factor c-Jun Affects Genes That Have Opposing Effects on Lipid Homeostasis in Mice J. Biol. Chem., July 6, 2007; 282(27): 19556 - 19564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Browman, M. E. Resek, L. D. Zajchowski, and S. M. Robbins Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER J. Cell Sci., August 1, 2006; 119(15): 3149 - 3160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Biddinger, M. Miyazaki, J. Boucher, J. M. Ntambi, and C. R. Kahn Leptin Suppresses Stearoyl-CoA Desaturase 1 by Mechanisms Independent of Insulin and Sterol Regulatory Element-Binding Protein-1c. Diabetes, July 1, 2006; 55(7): 2032 - 2041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kato, K. Sakaki, and K. Mihara Ubiquitin-proteasome-dependent degradation of mammalian ER stearoyl-CoA desaturase J. Cell Sci., June 1, 2006; 119(11): 2342 - 2353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mancini, M. Aebi, and A. Helenius Multiple Endoplasmic Reticulum-associated Pathways Degrade Mutant Yeast Carboxypeptidase Y in Mammalian Cells J. Biol. Chem., November 21, 2003; 278(47): 46895 - 46905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Heinemann, G. Korza, and J. Ozols A Plasminogen-like Protein Selectively Degrades Stearoyl-CoA Desaturase in Liver Microsomes J. Biol. Chem., October 31, 2003; 278(44): 42966 - 42975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Kim, M. Miyazaki, and J. M. Ntambi Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism J. Lipid Res., October 1, 2002; 43(10): 1750 - 1757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miyazaki, W. C. Man, and J. M. Ntambi Targeted Disruption of Stearoyl-CoA Desaturase1 Gene in Mice Causes Atrophy of Sebaceous and Meibomian Glands and Depletion of Wax Esters in the Eyelid J. Nutr., September 1, 2001; 131(9): 2260 - 2268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Kim, F. E. Gomez, B. G. Fox, and J. M. Ntambi Differential regulation of the stearoyl-CoA desaturase genes by thiazolidinediones in 3T3-L1 adipocytes J. Lipid Res., August 1, 2000; 41(8): 1310 - 1316. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Mziaut, G. Korza, and J. Ozols The N terminus of microsomal Delta 9 stearoyl-CoA desaturase contains the sequence determinant for its rapid degradation PNAS, August 1, 2000; 97(16): 8883 - 8888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Choi, Y.-C. Kim, Y.-B. Han, Y. Park, M. W. Pariza, and J. M. Ntambi The trans-10,cis-12 Isomer of Conjugated Linoleic Acid Downregulates Stearoyl-CoA Desaturase 1 Gene Expression in 3T3-L1 Adipocytes J. Nutr., August 1, 2000; 130(8): 1920 - 1924. [Abstract] [Full Text]
|
|
|
|
|
|
O. C. Trifan, R. M. Smith, B. D. Thompson, and T. Hla Overexpression of Cyclooxygenase-2 Induces Cell Cycle Arrest. EVIDENCE FOR A PROSTAGLANDIN-INDEPENDENT MECHANISM J. Biol. Chem., November 26, 1999; 274(48): 34141 - 34147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mziaut, G. Korza, A. R. Hand, C. Gerard, and J. Ozols Targeting Proteins to the Lumen of Endoplasmic Reticulum Using N-terminal Domains of 11beta -Hydroxysteroid Dehydrogenase and the 50-kDa Esterase J. Biol. Chem., May 14, 1999; 274(20): 14122 - 14129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyazaki, Y.-C. Kim, M. P. Gray-Keller, A. D. Attie, and J. M. Ntambi The Biosynthesis of Hepatic Cholesterol Esters and Triglycerides Is Impaired in Mice with a Disruption of the Gene for Stearoyl-CoA Desaturase 1 J. Biol. Chem., September 22, 2000; 275(39): 30132 - 30138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Umebayashi, R. Fukuda, A. Hirata, H. Horiuchi, A. Nakano, A. Ohta, and M. Takagi Activation of the Ras-cAMP Signal Transduction Pathway Inhibits the Proteasome-independent Degradation of Misfolded Protein Aggregates in the Endoplasmic Reticulum Lumen J. Biol. Chem., October 26, 2001; 276(44): 41444 - 41454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Fayadat, S. Siffroi-Fernandez, J. Lanet, and J.-L. Franc Degradation of Human Thyroperoxidase in the Endoplasmic Reticulum Involves Two Different Pathways Depending on the Folding State of the Protein J. Biol. Chem., May 19, 2000; 275(21): 15948 - 15954. [Abstract] [Full Text] [PDF]
|
|
