QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Palokangas, H. Articles by Saraste, J. Search for Related Content PubMed PubMed Citation Articles by Palokangas, H. Articles by Saraste, J.

Vol. 9, Issue 12, 3561-3578, December 1998

Retrograde Transport from the Pre-Golgi Intermediate Compartment and the Golgi Complex Is Affected by the Vacuolar H⁺-ATPase Inhibitor Bafilomycin A1

Harri Palokangas,^* Ming Ying,^* Kalervo Väänänen, and Jaakko Saraste^*

 ^*Department of Biochemistry and Molecular Biology, University of Bergen, Norway; and  Department of Anatomy and Biocenter, University of Oulu, Finland

The effect of the vacuolar H⁺-ATPase inhibitor bafilomycin A1 (Baf A1) on the localization of pre-Golgi intermediate compartment (IC) and Golgi marker proteins was used to study the role of acidification in the function of early secretory compartments. Baf A1 inhibited both brefeldin A- and nocodazole-induced retrograde transport of Golgi proteins to the endoplasmic reticulum (ER), whereas anterograde ER-to-Golgi transport remained largely unaffected. Furthermore, p58/ERGIC-53, which normally cycles between the ER, IC, and cis-Golgi, was arrested in pre-Golgi tubules and vacuoles, and the number of p58-positive ~80-nm Golgi (coatomer protein I) vesicles was reduced, suggesting that the drug inhibits the retrieval of the protein from post-ER compartments. In parallel, redistribution of -coatomer protein from the Golgi to peripheral pre-Golgi structures took place. The small GTPase rab1p was detected in short pre-Golgi tubules in control cells and was efficiently recruited to the tubules accumulating in the presence of Baf A1. In contrast, these tubules showed no enrichment of newly synthesized, anterogradely transported proteins, indicating that they participate in retrograde transport. These results suggest that the pre-Golgi structures contain an active H⁺-ATPase that regulates retrograde transport at the ER-Golgi boundary. Interestingly, although Baf A1 had distinct effects on peripheral pre-Golgi structures, only more central, p58-containing elements accumulated detectable amounts of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP), a marker for acidic compartments, raising the possibility that the lumenal pH of the pre-Golgi structures gradually changes in parallel with their translocation to the Golgi region.

---

   Corresponding author and present address: Department of Anatomy and Cell Biology, University of Bergen, Årstadveien 19, N-5009 Bergen, Norway. E-mail address: jaakko.saraste{at}pki.uib.no.

---

Molecular Biology of the Cell
Vol. 9, 3561-3578, December 1998
Copyright © 1998 by The American Society for Cell Biology

This article has been cited by other articles:

				S. M. Eswarappa, N. Basu, O. Joy, and D. Chakravortty Folimycin (concanamycin A) inhibits LPS-induced nitric oxide production and reduces surface localization of TLR4 in murine macrophages Innate Immunity, February 1, 2008; 14(1): 13 - 24. [Abstract] [PDF]

				S. Ivanov, A.-M. Dragoi, X. Wang, C. Dallacosta, J. Louten, G. Musco, G. Sitia, G. S. Yap, Y. Wan, C. A. Biron, et al. A novel role for HMGB1 in TLR9-mediated inflammatory responses to CpG-DNA Blood, September 15, 2007; 110(6): 1970 - 1981. [Abstract] [Full Text] [PDF]

				M. Oueslati, R. Hermosilla, E. Schonenberger, V. Oorschot, M. Beyermann, B. Wiesner, A. Schmidt, J. Klumperman, W. Rosenthal, and R. Schulein Rescue of a Nephrogenic Diabetes Insipidus-causing Vasopressin V2 Receptor Mutant by Cell-penetrating Peptides J. Biol. Chem., July 13, 2007; 282(28): 20676 - 20685. [Abstract] [Full Text] [PDF]

				J. Saraste and B. Goud Functional Symmetry of Endomembranes Mol. Biol. Cell, April 1, 2007; 18(4): 1430 - 1436. [Abstract] [Full Text] [PDF]

				T. Yokozeki, S. Wakatsuki, K. Hatsuzawa, R. A. Black, I. Wada, and A. Sehara-Fujisawa Meltrin {beta} (ADAM19) mediates ectodomain shedding of Neuregulin {beta}1 in the Golgi apparatus: fluorescence correlation spectroscopic observation of the dynamics of ectodomain shedding in living cells Genes Cells, March 1, 2007; 12(3): 329 - 343. [Abstract] [Full Text] [PDF]

				C. Appenzeller-Herzog and H.-P. Hauri The ER-Golgi intermediate compartment (ERGIC): in search of its identity and function J. Cell Sci., June 1, 2006; 119(11): 2173 - 2183. [Abstract] [Full Text] [PDF]

				R. Sannerud, M. Marie, C. Nizak, H. A. Dale, K. Pernet-Gallay, F. Perez, B. Goud, and J. Saraste Rab1 Defines a Novel Pathway Connecting the Pre-Golgi Intermediate Compartment with the Cell Periphery Mol. Biol. Cell, April 1, 2006; 17(4): 1514 - 1526. [Abstract] [Full Text] [PDF]

				N. Kokkonen, A. Rivinoja, A. Kauppila, M. Suokas, I. Kellokumpu, and S. Kellokumpu Defective Acidification of Intracellular Organelles Results in Aberrant Secretion of Cathepsin D in Cancer Cells J. Biol. Chem., September 17, 2004; 279(38): 39982 - 39988. [Abstract] [Full Text] [PDF]

				H. A. W. Tawfeek and A. B. Abou-Samra Important role for the V-type H+-ATPase and the Golgi apparatus in the recycling of PTH/PTHrP receptor Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E704 - E710. [Abstract] [Full Text] [PDF]

				A.-Q. Sun, N. Balasubramaniyan, C.-J. Liu, M. Shahid, and F. J. Suchy Association of the 16-kDa Subunit c of Vacuolar Proton Pump with the Ileal Na+-dependent Bile Acid Transporter: PROTEIN-PROTEIN INTERACTION AND INTRACELLULAR TRAFFICKING J. Biol. Chem., April 16, 2004; 279(16): 16295 - 16300. [Abstract] [Full Text] [PDF]

				C. Appenzeller-Herzog, A.-C. Roche, O. Nufer, and H.-P. Hauri pH-induced Conversion of the Transport Lectin ERGIC-53 Triggers Glycoprotein Release J. Biol. Chem., March 26, 2004; 279(13): 12943 - 12950. [Abstract] [Full Text] [PDF]

				H. Watabe, J. C. Valencia, K.-i. Yasumoto, T. Kushimoto, H. Ando, J. Muller, W. D. Vieira, M. Mizoguchi, E. Appella, and V. J. Hearing Regulation of Tyrosinase Processing and Trafficking by Organellar pH and by Proteasome Activity J. Biol. Chem., February 27, 2004; 279(9): 7971 - 7981. [Abstract] [Full Text] [PDF]

				D. Drecktrah, K. Chambers, E. L. Racoosin, E. B. Cluett, A. Gucwa, B. Jackson, and W. J. Brown Inhibition of a Golgi Complex Lysophospholipid Acyltransferase Induces Membrane Tubule Formation and Retrograde Trafficking Mol. Biol. Cell, August 1, 2003; 14(8): 3459 - 3469. [Abstract] [Full Text] [PDF]

				R. Halaban, R. S. Patton, E. Cheng, S. Svedine, E. S. Trombetta, M. L. Wahl, S. Ariyan, and D. N. Hebert Abnormal Acidification of Melanoma Cells Induces Tyrosinase Retention in the Early Secretory Pathway J. Biol. Chem., April 19, 2002; 277(17): 14821 - 14828. [Abstract] [Full Text] [PDF]

				H.-L. Hsieh, B. W. Schafer, J. A. Cox, and C. W. Heizmann S100A13 and S100A6 exhibit distinct translocation pathways in endothelial cells J. Cell Sci., January 8, 2002; 115(15): 3149 - 3158. [Abstract] [Full Text] [PDF]

				F. Gu and J. Gruenberg ARF1 Regulates pH-dependent COP Functions in the Early Endocytic Pathway J. Biol. Chem., March 10, 2000; 275(11): 8154 - 8160. [Abstract] [Full Text] [PDF]

				N. Perzov, H. Nelson, and N. Nelson Altered Distribution of the Yeast Plasma Membrane H+-ATPase as a Feature of Vacuolar H+-ATPase Null Mutants J. Biol. Chem., December 15, 2000; 275(51): 40088 - 40095. [Abstract] [Full Text] [PDF]