![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 2, 277-290, February 1998
v
3 Integrin
and
*Banting and Best Department of Medical Research and Department of
Biochemistry, University of Toronto, Toronto, Ontario M5G 1L6, Canada;
and
The cell adhesion molecule L1 is a potent inducer of neurite
outgrowth and it has been implicated in X-linked hydrocephalus and
related neurological disorders. To investigate the mechanisms of
neurite outgrowth stimulated by L1, attempts were made to identify the
neuritogenic sites in L1. Fusion proteins containing different segments
of the extracellular region of L1 were prepared and different neuronal
cells were assayed on substrate-coated fusion proteins. Interestingly,
both immunoglobulin (Ig)-like domains 2 and 6 (Ig2, Ig6) promoted
neurite outgrowth from dorsal root ganglion cells, whereas neural
retinal cells responded only to Ig2. L1 Ig2 contains a previously
identified homophilic binding site, whereas L1 Ig6 contains an
Arg-Gly-Asp (RGD) sequence. The neuritogenic activity of Ig6 was
abrogated by mutations in the RGD site. The addition of RGD-containing
peptides also inhibited the promotion of neurite outgrowth from dorsal
root ganglion cells by glutathione S-transferase-Ig6, implicating the involvement of an integrin. The monoclonal
antibody LM609 against Department of Immunology, The Scripps Research
Institute, La Jolla, California 92037
v
3
integrin, but not an anti-1 antibody, inhibited the neuritogenic effects of Ig6. These data thus provide the first evidence that the RGD motif in L1 Ig6 is capable of promoting neurite
outgrowth via interaction with the v3
integrin on neuronal cells.
This article has been cited by other articles:
|
|
 |
|
  L. Maddaluno, S. E. Verbrugge, C. Martinoli, G. Matteoli, A. Chiavelli, Y. Zeng, E. D. Williams, M. Rescigno, and U. Cavallaro The adhesion molecule L1 regulates transendothelial migration and trafficking of dendritic cells J. Exp. Med., March 16, 2009; 206(3): 623 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Gouveia, C. M. Gomes, M. Sousa, P. M. Alves, and J. Costa Kinetic Analysis of L1 Homophilic Interaction: ROLE OF THE FIRST FOUR IMMUNOGLOBULIN DOMAINS AND IMPLICATIONS ON BINDING MECHANISM J. Biol. Chem., October 17, 2008; 283(42): 28038 - 28047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. He, D. H. Hryciw, M. L. Carroll, S. A. Myers, A. K. Whitbread, S. Kumar, P. Poronnik, and J. D. Hooper The Ubiquitin-Protein Ligase Nedd4-2 Differentially Interacts with and Regulates Members of the Tweety Family of Chloride Ion Channels J. Biol. Chem., August 29, 2008; 283(35): 24000 - 24010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, J. Wu, I. Apostolova, M. Skup, A. Irintchev, S. Kugler, and M. Schachner Adeno-associated virus-mediated L1 expression promotes functional recovery after spinal cord injury Brain, April 1, 2007; 130(4): 954 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Silletti, M. Yebra, B. Perez, V. Cirulli, M. McMahon, and A. M. P. Montgomery Extracellular Signal-regulated Kinase (ERK)-dependent Gene Expression Contributes to L1 Cell Adhesion Molecule-dependent Motility and Invasion J. Biol. Chem., July 9, 2004; 279(28): 28880 - 28888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Itoh, L. Cheng, Y. Kamei, S. Fushiki, H. Kamiguchi, P. Gutwein, A. Stoeck, B. Arnold, P. Altevogt, and V. Lemmon Brain development in mice lacking L1-L1 homophilic adhesion J. Cell Biol., April 12, 2004; 165(1): 145 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nishimura, F. Yoshihara, T. Tojima, N. Ooashi, W. Yoon, K. Mikoshiba, V. Bennett, and H. Kamiguchi L1-dependent neuritogenesis involves ankyrinB that mediates L1-CAM coupling with retrograde actin flow J. Cell Biol., December 8, 2003; 163(5): 1077 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Buhusi, B. R. Midkiff, A. M. Gates, M. Richter, M. Schachner, and P. F. Maness Close Homolog of L1 Is an Enhancer of Integrin-mediated Cell Migration J. Biol. Chem., June 27, 2003; 278(27): 25024 - 25031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ramm, Y. Alexandrov, A. Cholewinski, Y. Cybuch, R. Nadon, and B. J. Soltys Automated Screening of Neurite Outgrowth J Biomol Screen, January 1, 2003; 8(1): 7 - 18. [Abstract] [PDF]
|
|
|
|
|
|
Y. Nakai and H. Kamiguchi Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner J. Cell Biol., December 23, 2002; 159(6): 1097 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Thelen, V. Kedar, A. K. Panicker, R.-S. Schmid, B. R. Midkiff, and P. F. Maness The Neural Cell Adhesion Molecule L1 Potentiates Integrin-Dependent Cell Migration to Extracellular Matrix Proteins J. Neurosci., June 15, 2002; 22(12): 4918 - 4931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. De Angelis, A. Watkins, M. Schafer, T. Brummendorf, S. Kenwrick, Y. Sakaki, and T. Ikemura Disease-associated mutations in L1 CAM interfere with ligand interactions and cell-surface expression Hum. Mol. Genet., January 1, 2002; 11(1): 1 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mechtersheimer, P. Gutwein, N. Agmon-Levin, A. Stoeck, M. Oleszewski, S. Riedle, R. Postina, F. Fahrenholz, M. Fogel, V. Lemmon, et al. Ectodomain shedding of L1 adhesion molecule promotes cell migration by autocrine binding to integrins J. Cell Biol., November 12, 2001; 155(4): 661 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Voura, R. A. Ramjeesingh, A. M.P. Montgomery, and C.-H. Siu Involvement of Integrin alpha vbeta 3 and Cell Adhesion Molecule L1 in Transendothelial Migration of Melanoma Cells Mol. Biol. Cell, September 1, 2001; 12(9): 2699 - 2710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Moulding, R. L. Martuza, and S. D. Rabkin Clinical Mutations in the L1 Neural Cell Adhesion Molecule Affect Cell-Surface Expression J. Neurosci., August 1, 2000; 20(15): 5696 - 5702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-S. Schmid, W. M. Pruitt, and P. F. Maness A MAP Kinase-Signaling Pathway Mediates Neurite Outgrowth on L1 and Requires Src-Dependent Endocytosis J. Neurosci., June 1, 2000; 20(11): 4177 - 4188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takei, T. A. Chan, F.-S. Wang, H. Deng, U. Rutishauser, and D. G. Jay The Neural Cell Adhesion Molecules L1 and NCAM-180 Act in Different Steps of Neurite Outgrowth J. Neurosci., November 1, 1999; 19(21): 9469 - 9479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Beer, M Oleszewski, P Gutwein, C Geiger, and P Altevogt Metalloproteinase-mediated release of the ectodomain of L1 adhesion molecule J. Cell Sci., January 8, 1999; 112(16): 2667 - 2675. [Abstract] [PDF]
|
|
