Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krieg, J.
Right arrow Articles by Hofsteenge, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krieg, J.
Right arrow Articles by Hofsteenge, J.

Vol. 9, Issue 2, 301-309, February 1998

Recognition Signal for C-Mannosylation of Trp-7 in RNase 2 Consists of Sequence Trp-x-x-Trp

Joachim Krieg, Steffen Hartmann, Anna Vicentini, Wolfgang Gläsner, Daniel Hess, and Jan Hofsteenge*

Friedrich Miescher-Institut, CH-4002 Basel, Switzerland

C2-alpha -Mannosyltryptophan was discovered in human RNase 2, an enzyme that occurs in eosinophils and is involved in host defense. It represents a novel way of attaching carbohydrate to a protein in addition to the well-known N- and O-glycosylations. The reaction is specific, as in RNase 2 Trp-7, but never Trp-10, which is modified. In this article, we address which structural features provide the specificity of the reaction. Expression of chimeras of RNase 2 and nonglycosylated RNase 4 and deletion mutants in HEK293 cells identified residues 1-13 to be sufficient for C-mannosylation. Site-directed mutagenesis revealed the sequence Trp-x-x-Trp, in which the first Trp becomes mannosylated, as the specificity determinant. The Trp residue at position +3 can be replaced by Phe, which reduces the efficiency of the reaction threefold. Interpretation of the data in the context of the three-dimensional structure of RNase 2 strongly suggests that the primary, rather than the tertiary, structure forms the determinant. The sequence motif occurs in 336 mammalian proteins currently present in protein databases. Two of these proteins were analyzed protein chemically, which showed partial C-glycosylation of recombinant human interleukin 12. The frequent occurrence of the protein recognition motif suggests that C-glycosides could be part of the structure of more proteins than assumed so far.

Molecular Biology of the Cell
Vol. 9, 301-309, February 1998
Copyright © 1998 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D. Hess, J. J. Keusch, S. A. L. Oberstein, R. C. M. Hennekam, and J. Hofsteenge
Peters Plus Syndrome Is a New Congenital Disorder of Glycosylation and Involves Defective O-Glycosylation of Thrombospondin Type 1 Repeats
J. Biol. Chem., March 21, 2008; 283(12): 7354 - 7360.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
K. Julenius
NetCGlyc 1.0: prediction of mammalian C-mannosylation sites
Glycobiology, August 1, 2007; 17(8): 868 - 876.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kozma, J. J. Keusch, B. Hegemann, K. B. Luther, D. Klein, D. Hess, R. S. Haltiwanger, and J. Hofsteenge
Identification and Characterization of abeta1,3-Glucosyltransferase That Synthesizes the Glc-beta1,3-Fuc Disaccharide on Thrombospondin Type 1 Repeats
J. Biol. Chem., December 1, 2006; 281(48): 36742 - 36751.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Li, L. Cui, D. L. Rock, and J. Li
Novel Glycosidic Linkage in Aedes aegypti Chorion Peroxidase: N-MANNOSYL TRYPTOPHAN
J. Biol. Chem., November 18, 2005; 280(46): 38513 - 38521.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
J. Perez-Vilar, S. H. Randell, and R. C. Boucher
C-Mannosylation of MUC5AC and MUC5B Cys subdomains
Glycobiology, April 1, 2004; 14(4): 325 - 337.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. V. Zeenko, L. A. Ryabova, A. S. Spirin, H. M. Rothnie, D. Hess, K. S. Browning, and T. Hohn
Eukaryotic Elongation Factor 1A Interacts with the Upstream Pseudoknot Domain in the 3' Untranslated Region of Tobacco Mosaic Virus RNA
J. Virol., May 3, 2002; 76(11): 5678 - 5691.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
R. G. Spiro
Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds
Glycobiology, April 1, 2002; 12(4): 43R - 56R.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Gonzalez de Peredo, D. Klein, B. Macek, D. Hess, J. Peter-Katalinic, and J. Hofsteenge
C-Mannosylation and O-Fucosylation of Thrombospondin Type 1 Repeats
Mol. Cell. Proteomics, January 1, 2002; 1(1): 11 - 18.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Anand, J. S. Rush, S. Ray, M.-A. Doucey, J. Weik, F. E. Ware, J. Hofsteenge, C. J. Waechter, and M. A. Lehrman
Requirement of the Lec35 Gene for All Known Classes of Monosaccharide-P-Dolichol-dependent Glycosyltransferase Reactions in Mammals
Mol. Biol. Cell, February 1, 2001; 12(2): 487 - 501.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
I. Beuvink, D. Hess, H. Flotow, J. Hofsteenge, B. Groner, and N. E. Hynes
Stat5a Serine Phosphorylation. SERINE 779 IS CONSTITUTIVELY PHOSPHORYLATED IN THE MAMMARY GLAND, AND SERINE 725 PHOSPHORYLATION INFLUENCES PROLACTIN-STIMULATED IN VITRO DNA BINDING ACTIVITY
J. Biol. Chem., March 31, 2000; 275(14): 10247 - 10255.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Billy, D. Hess, J. Hofsteenge, and W. Filipowicz
Characterization of the Adenylation Site in the RNA 3'-Terminal Phosphate Cyclase from Escherichia coli
J. Biol. Chem., December 3, 1999; 274(49): 34955 - 34960.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. A. Millward, D. Hess, and B. A. Hemmings
Ndr Protein Kinase Is Regulated by Phosphorylation on Two Conserved Sequence Motifs
J. Biol. Chem., November 26, 1999; 274(48): 33847 - 33850.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Hofsteenge, M. Blommers, D. Hess, A. Furmanek, and O. Miroshnichenko
The Four Terminal Components of the Complement System Are C-Mannosylated on Multiple Tryptophan Residues
J. Biol. Chem., November 12, 1999; 274(46): 32786 - 32794.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M.-A. Doucey, D. Hess, R. Cacan, and J. Hofsteenge
Protein C-Mannosylation Is Enzyme-catalysed and Uses Dolichyl-Phosphate-Mannose as a Precursor
Mol. Biol. Cell, February 1, 1998; 9(2): 291 - 300.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Hartmann and J. Hofsteenge
Properdin, the Positive Regulator of Complement, Is Highly C-Mannosylated
J. Biol. Chem., September 8, 2000; 275(37): 28569 - 28574.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Hofsteenge, K. G. Huwiler, B. Macek, D. Hess, J. Lawler, D. F. Mosher, and J. Peter-Katalinic
C-Mannosylation and O-Fucosylation of the Thrombospondin Type 1 Module
J. Biol. Chem., February 23, 2001; 276(9): 6485 - 6498.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. D. Leonidas, E. Boix, R. Prill, M. Suzuki, R. Turton, K. Minson, G. J. Swaminathan, R. J. Youle, and K. R. Acharya
Mapping the Ribonucleolytic Active Site of Eosinophil-derived Neurotoxin (EDN). HIGH RESOLUTION CRYSTAL STRUCTURES OF EDN COMPLEXES WITH ADENYLIC NUCLEOTIDE INHIBITORS
J. Biol. Chem., April 27, 2001; 276(18): 15009 - 15017.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Spada, H. Steen, C. Troedsson, T. Kallesoe, E. Spriet, M. Mann, and E. M. Thompson
Molecular Patterning of the Oikoplastic Epithelium of the Larvacean Tunicate Oikopleura dioica
J. Biol. Chem., June 1, 2001; 276(23): 20624 - 20632.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]