![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 3, 545-559, March 1998
Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany
DdLim, a multi-domain member of the cysteine-rich family of LIM
domain proteins, was isolated from Dictyostelium cells
where it localizes in lamellipodia and at sites of membrane ruffling. The transcription and expression of DdLim are developmentally regulated, and the timing of its increased association with the actin
cytoskeleton coincides with the acquisition in starved cells of a
motile, chemotactic behavior. Vegetative cells that overexpress DdLim
contain large lamella and exhibit ruffling at the cortex. The high
frequency of large, multinucleated mutant cells found in suspension
culture suggests that excess DdLim interferes with cytokinesis. DdLim
was also identified as a protein in a Dictyostelium cell
lysate that associated indirectly, but in a guanosine
triphosphate-dependent manner, with a GST-rac1 fusion protein. The data
presented suggest that DdLim acts as an adapter protein at the
cytoskeleton-membrane interface where it is involved in a
receptor-mediated rac1-signaling pathway that leads to actin
polymerization in lamellipodia and ultimately cell motility.
This article has been cited by other articles:
|
|
 |
|
  J. Dalous, E. Burghardt, A. Muller-Taubenberger, F. Bruckert, G. Gerisch, and T. Bretschneider Reversal of Cell Polarity and Actin-Myosin Cytoskeleton Reorganization under Mechanical and Chemical Stimulation Biophys. J., February 1, 2008; 94(3): 1063 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wender, E. Villalobo, and D. Mirelman EhLimA, a Novel LIM Protein, Localizes to the Plasma Membrane in Entamoeba histolytica Eukaryot. Cell, September 1, 2007; 6(9): 1646 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fajardo, M. Schleicher, A. Noegel, S. Bozzaro, S. Killinger, K. Heuner, J. Hacker, and M. Steinert Calnexin, calreticulin and cytoskeleton-associated proteins modulate uptake and growth of Legionella pneumophila in Dictyostelium discoideum Microbiology, September 1, 2004; 150(9): 2825 - 2835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Mackin, T. J. Sousou, and S. E. Erdman The PXL1 Gene of Saccharomyces cerevisiae Encodes a Paxillin-like Protein Functioning in Polarized Cell Growth Mol. Biol. Cell, April 1, 2004; 15(4): 1904 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chien, C. Y. Chung, S. Sukumaran, N. Osborne, S. Lee, C. Ellsworth, J. G. McNally, and R. A. Firtel The Dictyostelium LIM Domain-containing Protein LIM2 Is Essential for Proper Chemotaxis and Morphogenesis Mol. Biol. Cell, April 1, 2000; 11(4): 1275 - 1291. [Abstract] [Full Text]
|
|
|
|
 |
|
 M Dumontier, P Hocht, U Mintert, and J Faix Rac1 GTPases control filopodia formation, cell motility, endocytosis, cytokinesis and development in Dictyostelium J. Cell Sci., January 6, 2000; 113(12): 2253 - 2265. [Abstract] [PDF]
|
|
|
|
|
|
A. Noegel and M Schleicher The actin cytoskeleton of Dictyostelium: a story told by mutants J. Cell Sci., January 3, 2000; 113(5): 759 - 766. [Abstract] [PDF]
|
|
|
|
|
|
S. Stocker, M. Hiery, and G. Marriott Phototactic Migration of Dictyostelium Cells Is Linked to a New Type of Gelsolin-related Protein Mol. Biol. Cell, January 1, 1999; 10(1): 161 - 178. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Song, R. S. Maul, C. S. Gerbin, and D. D. Chang Inhibition of Anchorage-independent Growth of Transformed NIH3T3 Cells by Epithelial Protein Lost in Neoplasm (EPLIN) Requires Localization of EPLIN to Actin Cytoskeleton Mol. Biol. Cell, April 1, 2002; 13(4): 1408 - 1416. [Abstract] [Full Text] [PDF]
|
|
