![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 3, 653-670, March 1998
§
*Department of Biology, Division of Cellular and Molecular
Medicine, and the Howard Hughes Medical Institute, University of
California, San Diego La Jolla, California 92093-0668; and
ADP ribosylation factor (ARF) is thought to play a critical role in
recruiting coatomer (COPI) to Golgi membranes to drive transport
vesicle budding. Yeast strains harboring mutant COPI proteins exhibit
defects in retrograde Golgi to endoplasmic reticulum protein transport
and striking cargo-selective defects in anterograde endoplasmic
reticulum to Golgi protein transport. To determine whether
arf mutants exhibit similar phenotypes, the anterograde transport kinetics of multiple cargo proteins were examined in arf mutant cells, and, surprisingly, both COPI-dependent
and COPI-independent cargo proteins exhibited comparable defects.
Retrograde dilysine-mediated transport also appeared to be inefficient
in the arf mutants, and coatomer mutants with no
detectable anterograde transport defect exhibited a synthetic growth
defect when combined with arf1Department of Molecular Biology, Vanderbilt University,
Nashville, Tennessee 37235
, supporting a role for
ARF in retrograde transport. Remarkably, we found that early and medial
Golgi glycosyltransferases localized to abnormally large ring-shaped
structures. The endocytic marker FM4-64 also stained similar, but
generally larger ring-shaped structures en route from the plasma
membrane to the vacuole in arf mutants. Brefeldin A
similarly perturbed endosome morphology and also inhibited transport of
FM4-64 from endosomal structures to the vacuole. Electron microscopy
of arf mutant cells revealed the presence of what appear
to be hollow spheres of interconnected membrane tubules which likely
correspond to the fluorescent ring structures. Together, these
observations indicate that organelle morphology is significantly more
affected than transport in the arf mutants, suggesting a
fundamental role for ARF in regulating membrane dynamics. Possible
mechanisms for producing this dramatic morphological change in
intracellular organelles and its relation to the function of ARF in
coat assembly are discussed.
This article has been cited by other articles:
|
|
 |
|
  L. Zhang, M. Huang, and E. Harsay A Chemical Genetic Screen for Modulators of Exocytic Transport Identifies Inhibitors of a Transport Mechanism Linked to GTR2 Function Eukaryot. Cell, January 1, 2010; 9(1): 116 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yuksel and A. R. Memon Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules J. Exp. Bot., October 9, 2008; (2008) ern223v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Brown, A. B. Wolfe, D. Cui, and H.-L. Chiang The Vacuolar Import and Degradation Pathway Merges with the Endocytic Pathway to Deliver Fructose-1,6-bisphosphatase to the Vacuole for Degradation J. Biol. Chem., September 19, 2008; 283(38): 26116 - 26127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Matheson, S. L. Hanton, M. Rossi, M. Latijnhouwers, G. Stefano, L. Renna, and F. Brandizzi Multiple Roles of ADP-Ribosylation Factor 1 in Plant Cells Include Spatially Regulated Recruitment of Coatomer and Elements of the Golgi Matrix Plant Physiology, April 1, 2007; 143(4): 1615 - 1627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gabriely, R. Kama, and J. E. Gerst Involvement of Specific COPI Subunits in Protein Sorting from the Late Endosome to the Vacuole in Yeast Mol. Cell. Biol., January 15, 2007; 27(2): 526 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-W. Liu, S.-W. Lee, and F.-J. S. Lee Arl1p is involved in transport of the GPI-anchored protein Gas1p from the late Golgi to the plasma membrane J. Cell Sci., September 15, 2006; 119(18): 3845 - 3855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xiao, L. S. Kim, and T. R. Graham Dissection of Swa2p/Auxilin Domain Requirements for Cochaperoning Hsp70 Clathrin-uncoating Activity In Vivo Mol. Biol. Cell, July 1, 2006; 17(7): 3281 - 3290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Volpicelli-Daley, Y. Li, C.-J. Zhang, and R. A. Kahn Isoform-selective Effects of the Depletion of ADP-Ribosylation Factors 1-5 on Membrane Traffic Mol. Biol. Cell, October 1, 2005; 16(10): 4495 - 4508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Gebbie, J. E. Burn, C. H. Hocart, and R. E. Williamson Genes encoding ADP-ribosylation factors in Arabidopsis thaliana L. Heyn.; genome analysis and antisense suppression J. Exp. Bot., April 1, 2005; 56(414): 1079 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu and B. Scheres Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 Function in Epidermal Cell Polarity PLANT CELL, February 1, 2005; 17(2): 525 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Cox, R. J Mason-Gamer, C. L. Jackson, and N. Segev Phylogenetic Analysis of Sec7-Domain-containing Arf Nucleotide Exchangers Mol. Biol. Cell, April 1, 2004; 15(4): 1487 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantalat, S.-K. Park, Z. Hua, K. Liu, R. Gobin, A. Peyroche, A. Rambourg, T. R. Graham, and C. L. Jackson The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae J. Cell Sci., February 15, 2004; 117(5): 711 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Hua and T. R. Graham Requirement for Neo1p in Retrograde Transport from the Golgi Complex to the Endoplasmic Reticulum Mol. Biol. Cell, December 1, 2003; 14(12): 4971 - 4983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Couchy, S. Bolte, M.-T. Crosnier, S. Brown, and B. Satiat-Jeunemaitre Identification and localization of a {beta}-COP-like protein involved in the morphodynamics of the plant Golgi apparatus J. Exp. Bot., September 1, 2003; 54(390): 2053 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantalat, R. Courbeyrette, F. Senic-Matuglia, C. L. Jackson, B. Goud, and A. Peyroche A Novel Golgi Membrane Protein Is a Partner of the ARF Exchange Factors Gea1p and Gea2p Mol. Biol. Cell, June 1, 2003; 14(6): 2357 - 2371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nebenfuhr, C. Ritzenthaler, and D. G. Robinson Brefeldin A: Deciphering an Enigmatic Inhibitor of Secretion Plant Physiology, November 1, 2002; 130(3): 1102 - 1108. [Full Text] [PDF]
|
|
|
|
|
|
F. Baluska, A. Hlavacka, J. Samaj, K. Palme, D. G. Robinson, T. Matoh, D. W. McCurdy, D. Menzel, and D. Volkmann F-Actin-Dependent Endocytosis of Cell Wall Pectins in Meristematic Root Cells. Insights from Brefeldin A-Induced Compartments Plant Physiology, September 1, 2002; 130(1): 422 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Boman, P. D. Salo, M. J. Hauglund, N. L. Strand, S. J. Rensink, and O. Zhdankina ADP-Ribosylation Factor (ARF) Interaction Is Not Sufficient for Yeast GGA Protein Function or Localization Mol. Biol. Cell, September 1, 2002; 13(9): 3078 - 3095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yu and M. G. Roth Casein Kinase I Regulates Membrane Binding by ARF GAP1 Mol. Biol. Cell, August 1, 2002; 13(8): 2559 - 2570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Lee, M. K. Min, Y. J. Lee, J. B. Jin, D. H. Shin, D. H. Kim, K.-H. Lee, and I. Hwang ADP-Ribosylation Factor 1 of Arabidopsis Plays a Critical Role in Intracellular Trafficking and Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis Plant Physiology, August 1, 2002; 129(4): 1507 - 1520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jochum, D. Jackson, H. Schwarz, R. Pipkorn, and B. Singer-Kruger Yeast Ysl2p, Homologous to Sec7 Domain Guanine Nucleotide Exchange Factors, Functions in Endocytosis and Maintenance of Vacuole Integrity and Interacts with the Arf-Like Small GTPase Arl1p Mol. Cell. Biol., July 1, 2002; 22(13): 4914 - 4928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Yanagisawa, J. Marchena, Z. Xie, X. Li, P. P. Poon, R. A. Singer, G. C. Johnston, P. A. Randazzo, and V. A. Bankaitis Activity of Specific Lipid-regulated ADP Ribosylation Factor-GTPase-activating Proteins Is Required for Sec14p-dependent Golgi Secretory Function in Yeast Mol. Biol. Cell, July 1, 2002; 13(7): 2193 - 2206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Click, T. Stearns, and D. Botstein Systematic Structure-Function Analysis of the Small GTPase Arf1 in Yeast Mol. Biol. Cell, May 1, 2002; 13(5): 1652 - 1664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Peyroche, R. Courbeyrette, A. Rambourg, and C. L. Jackson The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast J. Cell Sci., March 8, 2002; 114(12): 2241 - 2253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Emans, S. Zimmermann, and R. Fischer Uptake of a Fluorescent Marker in Plant Cells Is Sensitive to Brefeldin A and Wortmannin PLANT CELL, January 1, 2002; 14(1): 71 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. de Figueiredo, A. Doody, R. S. Polizotto, D. Drecktrah, S. Wood, M. Banta, M. S. Strang, and W. J. Brown Inhibition of Transferrin Recycling and Endosome Tubulation by Phospholipase A2 Antagonists J. Biol. Chem., December 7, 2001; 276(50): 47361 - 47370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chaudhuri, A. Kumar, and M. Berger Association of ARF and Rabs with complement receptor Type-1 storage vesicles in human neutrophils J. Leukoc. Biol., October 1, 2001; 70(4): 669 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. W. Rossanese, C. A. Reinke, B. J. Bevis, A. T. Hammond, I. B. Sears, J. O'Connor, and B. S. Glick A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae J. Cell Biol., April 2, 2001; 153(1): 47 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yahara, T. Ueda, K. Sato, and A. Nakano Multiple Roles of Arf1 GTPase in the Yeast Exocytic and Endocytic Pathways Mol. Biol. Cell, January 1, 2001; 12(1): 221 - 238. [Abstract] [Full Text]
|
|
|
|
|
|
A. Audhya, M. Foti, and S. D. Emr Distinct Roles for the Yeast Phosphatidylinositol 4-Kinases, Stt4p and Pik1p, in Secretion, Cell Growth, and Organelle Membrane Dynamics Mol. Biol. Cell, August 1, 2000; 11(8): 2673 - 2689. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Martin, J. Hidalgo, J. L. Rosa, P. Crottet, and A. Velasco Effect of Protein Kinase A Activity on the Association of ADP-ribosylation Factor 1 to Golgi Membranes J. Biol. Chem., June 16, 2000; 275(25): 19050 - 19059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gu and J. Gruenberg ARF1 Regulates pH-dependent COP Functions in the Early Endocytic Pathway J. Biol. Chem., March 10, 2000; 275(11): 8154 - 8160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Chen, M. F. Ingram, P. H. Rosal, and T. R. Graham Role for Drs2p, a P-Type Atpase and Potential Aminophospholipid Translocase, in Yeast Late Golgi Function J. Cell Biol., December 13, 1999; 147(6): 1223 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jones, G. Jedd, R. A. Kahn, A. Franzusoff, F. Bartolini, and N. Segev Genetic Interactions in Yeast Between Ypt GTPases and Arf Guanine Nucleotide Exchangers Genetics, August 1, 1999; 152(4): 1543 - 1556. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Sata, J. Moss, and M. Vaughan Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors PNAS, March 16, 1999; 96(6): 2752 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-F. Huang, L.-M. Buu, W.-L. Yu, and F.-J. S. Lee Characterization of a Novel ADP-ribosylation Factor-like Protein (yARL3) in Saccharomyces cerevisiae J. Biol. Chem., February 5, 1999; 274(6): 3819 - 3827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Reynolds, B. Diane Hopkins, M. R. Lyons, and T. R. Graham The High Osmolarity Glycerol Response (HOG) MAP Kinase Pathway Controls Localization of a Yeast Golgi Glycosyltransferase J. Cell Biol., November 16, 1998; 143(4): 935 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Chen and T. R. Graham An arf1{Delta} Synthetic Lethal Screen Identifies a New Clathrin Heavy Chain Conditional Allele That Perturbs Vacuolar Protein Transport in Saccharomyces cerevisiae Genetics, October 1, 1998; 150(2): 577 - 589. [Abstract] [Full Text]
|
|
|
|
|
|
C. E. Ooi, E. C. Dell'Angelica, and J. S. Bonifacino ADP-Ribosylation Factor 1 (ARF1) Regulates Recruitment of the AP-3 Adaptor Complex to Membranes J. Cell Biol., July 27, 1998; 142(2): 391 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Poon, S. F. Nothwehr, R. A. Singer, and G. C. Johnston The Gcs1 and Age2 ArfGAP proteins provide overlapping essential function for transport from the yeast trans-Golgi network J. Cell Biol., December 24, 2001; 155(7): 1239 - 1250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Stefan, A. Audhya, and S. D. Emr The Yeast Synaptojanin-like Proteins Control the Cellular Distribution of Phosphatidylinositol (4,5)-Bisphosphate Mol. Biol. Cell, February 1, 2002; 13(2): 542 - 557. [Abstract] [Full Text] [PDF]
|
|
