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Vol. 9, Issue 4, 749-757, April 1998
Departments of
*Biochemistry and
Gene targeting techniques and early mouse embryos have been used to
produce immortalized fibroblasts genetically deficient in phospholipase
C (PLC)-Medicine, Vanderbilt
University School of Medicine, Nashville, Tennessee 37232-0146
1, a ubiquitous tyrosine kinase substrate. Plcg1
/ embryos die at embryonic day 9;
however, cells derived from these embryos proliferate as well as cells
from Plcg1+/+ embryos. The null cells do
grow to a higher saturation density in serum-containing media, as their
capacity to spread out is decreased compared with that of wild-type
cells. In terms of epidermal growth factor receptor activation and
internalization, or growth factor induction of mitogen-activated
protein kinase, c-fos, or DNA synthesis in quiescent
cells, PLcg1/ cells respond equivalently
to PLcg1+/+ cells. Also, null cells are able
to migrate effectively in a wounded monolayer. Therefore, immortalized
fibroblasts do not require PLC-1 for many responses to growth
factors.
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