Spc98p Directs the Yeast gamma -Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body

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Vol. 9, Issue 4, 775-793, April 1998

Spc98p Directs the Yeast $gamma$ -Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body

Gislene Pereira,^* Michael Knop,^* and Elmar Schiebel^*

Max-Planck Institut für Biochemie, 82152 Martinsried, Germany

In the yeast Saccharomyces cerevisiae, microtubules are organized by the spindle pole body (SPB), which is embedded in thenuclear envelope. Microtubule organization requires the $gamma$ -tubulincomplex containing the $gamma$ -tubulin Tub4p, Spc98p, and Spc97p. TheTub4p complex is associated with cytoplasmic and nuclear substructuresof the SPB, which organize the cytoplasmic and nuclear microtubules.Here we present evidence that the Tub4p complex assembles in thecytoplasm and then either binds to the cytoplasmic side of theSPB or is imported into the nucleus followed by binding to thenuclear side of the SPB. Nuclear import of the Tub4p complex ismediated by the essential nuclear localization sequence of Spc98p.Our studies also indicate that Spc98p in the Tub4p complex isphosphorylated at the nuclear, but not at the cytoplasmic, sideof the SPB. This phosphorylation is cell cycle dependent and occursafter SPB duplication and nucleation of microtubules by the newSPB and therefore may have a role in mitotic spindle function.In addition, activation of the mitotic checkpoint stimulates Spc98pphosphorylation. The kinase Mps1p, which functions in SPB duplicationand mitotic checkpoint control, seems to be involved in Spc98pphosphorylation. Our results also suggest that the nuclear andcytoplasmic Tub4p complexes are regulated differently.

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				T. Sutani, T. Yuasa, T. Tomonaga, N. Dohmae, K. Takio, and M. Yanagida Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4 Genes & Dev., September 1, 1999; 13(17): 2271 - 2283. [Abstract] [Full Text]

				K. L. Loveland, D. Herszfeld, B. Chu, E. Rames, E. Christy, L. J. Briggs, R. Shakri, D. M. de Kretser, and D. A. Jans Novel Low Molecular Weight Microtubule-associated Protein-2 Isoforms Contain a Functional Nuclear Localization Sequence J. Biol. Chem., July 2, 1999; 274(27): 19261 - 19268. [Abstract] [Full Text] [PDF]

				M. d. l. C. Muñoz-Centeno, S. McBratney, A. Monterrosa, B. Byers, C. Mann, and M. Winey Saccharomyces cerevisiae MPS2 Encodes a Membrane Protein Localized at the Spindle Pole Body and the Nuclear Envelope Mol. Biol. Cell, July 1, 1999; 10(7): 2393 - 2406. [Abstract] [Full Text]

Spc98p Directs the Yeast -Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body

Spc98p Directs the Yeast $gamma$ -Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body

				B. D. Manning, J. G. Barrett, J. A. Wallace, H. Granok, and M. Snyder Differential Regulation of the Kar3p Kinesin-related Protein by Two Associated Proteins, Cik1p and Vik1p J. Cell Biol., March 22, 1999; 144(6): 1219 - 1233. [Abstract] [Full Text] [PDF]

				T. Nguyen, D. B.N. Vinh, D. K. Crawford, and T. N. Davis A Genetic Analysis of Interactions with Spc110p Reveals Distinct Functions of Spc97p and Spc98p, Components of the Yeast gamma -Tubulin Complex Mol. Biol. Cell, August 1, 1998; 9(8): 2201 - 2216. [Abstract] [Full Text]

				D. B. Friedman, J. W. Kern, B. J. Huneycutt, D. B. N. Vinh, D. K. Crawford, E. Steiner, D. Scheiltz, J. Yates III, K. A. Resing, N. G. Ahn, et al. Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain J. Biol. Chem., May 18, 2001; 276(21): 17958 - 17967. [Abstract] [Full Text] [PDF]