![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 4, 795-807, April 1998
and
*Department of Anatomy, University of California, San Francisco,
California 94143-0750; and
Differentiation of trophoblast giant cells in the rodent placenta
is accompanied by exit from the mitotic cell cycle and onset of
endoreduplication. Commitment to giant cell differentiation is under
developmental control, involving down-regulation of Id1 and Id2, concomitant with up-regulation of the basic
helix-loop-helix factor Hxt and acquisition of increased
adhesiveness. Endoreduplication disrupts the alternation of DNA
synthesis and mitosis that maintains euploid DNA content during
proliferation. To determine how the mammalian endocycle is regulated,
we examined the expression of the cyclins and cyclin-dependent kinases
during the transition from replication to endoreduplication in the
Rcho-1 rat choriocarcinoma cell line. We cultured these cells under
conditions that gave relatively synchronous endoreduplication. This
allowed us to study the events that occur during the transition from
the mitotic cycle to the first endocycle. With giant cell
differentiation, the cells switched cyclin D isoform expression from D3
to D1 and altered several checkpoint functions, acquiring a relative
insensitivity to DNA-damaging agents and a coincident serum
independence. The initiation of S phase during endocycles appeared to
involve cycles of synthesis of cyclins E and A, and termination of S
was associated with abrupt loss of cyclin A and E. Both cyclins were
absent from gap phase cells, suggesting that their degradation may be
necessary to allow reinitiation of the endocycle. The arrest of the
mitotic cycle at the onset of endoreduplication was associated with a failure to assemble cyclin B/p34cdk1 complexes during the
first endocycle. In subsequent endocycles, cyclin B expression was
suppressed. Together these data suggest several points at which cell
cycle regulation could be targeted to shift cells from a mitotic to an
endoreduplicative cycle.
Samuel Lunenfeld Research
Institute Mount Sinai Hospital and Departments of Obstetrics and
Gynecology, and Molecular and Medical Genetics, University of Toronto,
Toronto, Ontario M5G 1X5, Canada
This article has been cited by other articles:
|
|
 |
|
  K. Narbonne-Reveau, S. Senger, M. Pal, A. Herr, H. E. Richardson, M. Asano, P. Deak, and M. A. Lilly APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle Development, April 15, 2008; 135(8): 1451 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Asanoma, H. Kato, S. Yamaguchi, C. H. Shin, Z.-P. Liu, K. Kato, T. Inoue, Y. Miyanari, K. Yoshikawa, K. Sonoda, et al. HOP/NECC1, A Novel Regulator of Mouse Trophoblast Differentiation J. Biol. Chem., August 17, 2007; 282(33): 24065 - 24074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Arias and J. C. Walter Strength in numbers: preventing rereplication via multiple mechanisms in eukaryotic cells Genes & Dev., March 1, 2007; 21(5): 497 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Gultice, K. L. Selesniemi, and T. L. Brown Hypoxia Inhibits Differentiation of Lineage-Specific Rcho-1 Trophoblast Giant Cells Biol Reprod, June 1, 2006; 74(6): 1041 - 1050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Rutland, M. Mukhopadhyay, S. Underwood, N. Clyde, T. M. Mayhew, and C. A. Mitchell Induction of Intrauterine Growth Restriction by Reducing Placental Vascular Growth with the Angioinhibin TNP-470 Biol Reprod, December 1, 2005; 73(6): 1164 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verkest, C.-L. d. O. Manes, S. Vercruysse, S. Maes, E. Van Der Schueren, T. Beeckman, P. Genschik, M. Kuiper, D. Inze, and L. De Veylder The Cyclin-Dependent Kinase Inhibitor KRP2 Controls the Onset of the Endoreduplication Cycle during Arabidopsis Leaf Development through Inhibition of Mitotic CDKA;1 Kinase Complexes PLANT CELL, June 1, 2005; 17(6): 1723 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Durbarry, I. Vizir, and D. Twell Male Germ Line Development in Arabidopsis. duo pollen Mutants Reveal Gametophytic Regulators of Generative Cell Cycle Progression Plant Physiology, January 1, 2005; 137(1): 297 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang Molecular Cues to Implantation Endocr. Rev., June 1, 2004; 25(3): 341 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Laronne, S. Rotkopf, A. Hellman, Y. Gruenbaum, A. C.G. Porter, and M. Brandeis Synchronization of Interphase Events Depends neither on Mitosis nor on cdk1 Mol. Biol. Cell, September 1, 2003; 14(9): 3730 - 3740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Cortes and N. Pastor Induction of endoreduplication by topoisomerase II catalytic inhibitors Mutagenesis, March 1, 2003; 18(2): 105 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kamei, S. R. Jones, B. M. Chapman, K. L. MCGonigle, G. Dai, and M. J. Soares The Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Modulates the Endocrine Differentiation of Trophoblast Cells Mol. Endocrinol., July 1, 2002; 16(7): 1469 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Cross, L. Anson-Cartwright, and I. C. Scott Transcription Factors Underlying the Development and Endocrine Functions of the Placenta Recent Prog. Horm. Res., January 1, 2002; 57(1): 221 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tateishi, M. Omata, K. Tanaka, and T. Chiba The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice J. Cell Biol., November 12, 2001; 155(4): 571 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ballester, J. Frampton, N. Vilaboa, and C. Cales Heterologous Expression of the Transcriptional Regulator Escargot Inhibits Megakaryocytic Endomitosis J. Biol. Chem., November 9, 2001; 276(46): 43413 - 43418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Rooney Cell Cycle Attenuation by p120E4F Is Accompanied by Increased Mitotic Dysfunction Cell Growth Differ., October 1, 2001; 12(10): 505 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Larkins, B. P. Dilkes, R. A. Dante, C. M. Coelho, Y.-m. Woo, and Y. Liu Investigating the hows and whys of DNA endoreduplication J. Exp. Bot., February 1, 2001; 52(355): 183 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Genbacev, M. T. McMaster, and S. J. Fisher A Repertoire of Cell Cycle Regulators Whose Expression Is Coordinated with Human Cytotrophoblast Differentiation Am. J. Pathol., October 1, 2000; 157(4): 1337 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hattori, T. C. Davies, L. Anson-Cartwright, and J. C. Cross Periodic Expression of the Cyclin-dependent Kinase Inhibitor p57Kip2 in Trophoblast Giant Cells Defines a G2-like Gap Phase of the Endocycle Mol. Biol. Cell, March 1, 2000; 11(3): 1037 - 1045. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. R. Jones, B. F. Kimler, W. M. Justice, and V. Rider Transit of Normal Rat Uterine Stromal Cells through G1 Phase of the Cell Cycle Requires Progesterone-Growth Factor Interactions Endocrinology, February 1, 2000; 141(2): 637 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Singer, M. Gurian-West, B. Clurman, and J. M. Roberts Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells Genes & Dev., September 15, 1999; 13(18): 2375 - 2387. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Sun, B. P. Dilkes, C. Zhang, R. A. Dante, N. P. Carneiro, K. S. Lowe, R. Jung, W. J. Gordon-Kamm, and B. A. Larkins Characterization of maize (Zea mays L.) Wee1 and its activity in developing endosperm PNAS, March 30, 1999; 96(7): 4180 - 4185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tanaka, T. Kunath, A. Hadjantonakis, A. Nagy, and J. Rossant Promotion of Trophoblast Stem Cell Proliferation by FGF4 Science, December 11, 1998; 282(5396): 2072 - 2075. [Abstract] [Full Text]
|
|
