Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Obermann, W. M.J.
Right arrow Articles by Fürst, D. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Obermann, W. M.J.
Right arrow Articles by Fürst, D. O.

Vol. 9, Issue 4, 829-840, April 1998

Mapping of a Myosin-binding Domain and a Regulatory Phosphorylation Site in M-Protein, a Structural Protein of the Sarcomeric M Band

Wolfgang M.J. Obermann,* Peter F.M. van der Ven,*dagger Frank Steiner,* Klaus Weber,* and Dieter O. Fürstdagger Dagger

 *Max-Planck-Institute for Biophysical Chemistry, Department of Biochemistry, D-37077 Göttingen, Germany; and  dagger University of Potsdam, Department of Cell Biology, D-14471 Potsdam, Germany

The myofibrils of cross-striated muscle fibers contain in their M bands cytoskeletal proteins whose main function seems to be the stabilization of the three-dimensional arrangement of thick filaments. We identified two immunoglobin domains (Mp2-Mp3) of M-protein as a site binding to the central region of light meromyosin. This binding is regulated in vitro by phosphorylation of a single serine residue (Ser76) in the immediately adjacent amino-terminal domain Mp1. M-protein phosphorylation by cAMP-dependent kinase A inhibits binding to myosin LMM. Transient transfection studies of cultured cells revealed that the myosin-binding site seems involved in the targeting of M-protein to its location in the myofibril. Using the same method, a second myofibril-binding site was uncovered in domains Mp9-Mp13. These results support the view that specific phosphorylation events could be also important for the control of sarcomeric M band formation and remodeling.

Molecular Biology of the Cell
Vol. 9, 829-840, April 1998
Copyright © 1998 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
N. Bit-Avragim, N. Hellwig, F. Rudolph, C. Munson, D. Y. S. Stainier, and S. Abdelilah-Seyfried
Divergent polarization mechanisms during vertebrate epithelial development mediated by the Crumbs complex protein Nagie oko
J. Cell Sci., August 1, 2008; 121(15): 2503 - 2510.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Woods, A. Farrall, C. Procko, and M. L. Whitelaw
The bHLH/Per-Arnt-Sim transcription factor SIM2 regulates muscle transcript myomesin2 via a novel, non-canonical E-box sequence
Nucleic Acids Res., June 1, 2008; 36(11): 3716 - 3727.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. J. Potthoff, M. A. Arnold, J. McAnally, J. A. Richardson, R. Bassel-Duby, and E. N. Olson
Regulation of Skeletal Muscle Sarcomere Integrity and Postnatal Muscle Function by Mef2c
Mol. Cell. Biol., December 1, 2007; 27(23): 8143 - 8151.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
T. Lowe, R. A. Kley, P. F.M. van der Ven, M. Himmel, A. Huebner, M. Vorgerd, and D. O. Furst
The pathomechanism of filaminopathy: altered biochemical properties explain the cellular phenotype of a protein aggregation myopathy
Hum. Mol. Genet., June 1, 2007; 16(11): 1351 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Kontrogianni-Konstantopoulos, D. H. Catino, J. C. Strong, W. R. Randall, and R. J. Bloch
Obscurin regulates the organization of myosin into A bands
Am J Physiol Cell Physiol, July 1, 2004; 287(1): C209 - C217.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. H. Andrade, A. P. Merriam, W. Guo, G. Cheng, C. A. McMullen, K. Hayess, P. F. M. van der Ven, and J. D. Porter
Paradoxical absence of M lines and downregulation of creatine kinase in mouse extraocular muscle
J Appl Physiol, August 1, 2003; 95(2): 692 - 699.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
P. F.M. van der Ven, S. Wiesner, P. Salmikangas, D. Auerbach, M. Himmel, S. Kempa, K. Haye{beta}, D. Pacholsky, A. Taivainen, R. Schroder, et al.
Indications for a Novel Muscular Dystrophy Pathway: {gamma}-Filamin, the Muscle-specific Filamin Isoform, Interacts with Myotilin
J. Cell Biol., October 9, 2000; 151(2): 235 - 248.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. van der Ven, J. Bartsch, M Gautel, H Jockusch, and D. Furst
A functional knock-out of titin results in defective myofibril assembly
J. Cell Sci., January 4, 2000; 113(8): 1405 - 1414.
[Abstract] [PDF]

Home page
 Mol. Biol. CellHome page
D. Auerbach, S. Bantle, S. Keller, V. Hinderling, M. Leu, E. Ehler, and J.-C. Perriard
Different Domains of the M-Band Protein Myomesin Are Involved in Myosin Binding and M-Band Targeting
Mol. Biol. Cell, May 1, 1999; 10(5): 1297 - 1308.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]