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Vol. 9, Issue 4, 931-943, April 1998
Centre de Génétique Moléculaire, Centre National
de la Recherche Scientifique, 91198 Gif-sur-Yvette, France
Microinjection at high copy number of plasmids containing only the
coding region of a gene into the Paramecium somatic
macronucleus led to a marked reduction in the expression of the
corresponding endogenous gene(s). The silencing effect, which is stably
maintained throughout vegetative growth, has been observed for all
Paramecium genes examined so far: a single-copy gene
(ND7), as well as members of multigene families (centrin
genes and trichocyst matrix protein genes) in which all closely related
paralogous genes appeared to be affected. This phenomenon may be
related to posttranscriptional gene silencing in transgenic plants and
quelling in Neurospora and allows the efficient creation
of specific mutant phenotypes thus providing a potentially powerful
tool to study gene function in Paramecium. For the two
multigene families that encode proteins that coassemble to build up
complex subcellular structures the analysis presented herein provides
the first experimental evidence that the members of these gene families
are not functionally redundant.
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