![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 5, 1037-1051, May 1998
Department of Anatomy and Cellular Biology, Tufts University
Medical School, Boston, Massachusetts 02111
Previously, we identified the heavy chain of ferritin as a
developmentally regulated nuclear protein of embryonic chicken corneal
epithelial cells. The nuclear ferritin is assembled into a
supramolecular form indistinguishable from the cytoplasmic form of
ferritin found in other cell types and thus most likely has iron-sequestering capabilities. Free iron, via the Fenton reaction, is
known to exacerbate UV-induced and other oxidative damage to cellular
components, including DNA. Since corneal epithelial cells are
constantly exposed to UV light, we hypothesized that the nuclear ferritin might protect the DNA of these cells from free radical damage.
To test this possibility, primary cultures of cells from corneal
epithelium and stroma, and from skin epithelium and stroma, were UV
irradiated, and DNA strand breaks were detected by an in situ 3'-end
labeling method. Corneal epithelial cells without nuclear ferritin were
also examined. We observed that the corneal epithelial cells with
nuclear ferritin had significantly less DNA breakage than other cell
types examined. Furthermore, increasing the iron concentration of the
culture medium exacerbated the generation of UV-induced DNA strand
breaks in corneal and skin fibroblasts, but not in the corneal
epithelial cells. Most convincingly, corneal epithelial cells in which
the expression of nuclear ferritin was inhibited became much more
susceptible to UV-induced DNA damage. Therefore, it seems that corneal
epithelial cells have evolved a novel, nuclear ferritin-based mechanism
for protecting their DNA against UV damage.
This article has been cited by other articles:
|
|
 |
|
  N. Lassen, J. B. Bateman, T. Estey, J. R. Kuszak, D. W. Nees, J. Piatigorsky, G. Duester, B. J. Day, J. Huang, L. M. Hines, et al. Multiple and Additive Functions of ALDH3A1 and ALDH1A1: CATARACT PHENOTYPE AND OCULAR OXIDATIVE DAMAGE IN Aldh3a1(-/-)/Aldh1a1(-/-) KNOCK-OUT MICE J. Biol. Chem., August 31, 2007; 282(35): 25668 - 25676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fisher, K. Devraj, J. Ingram, B. Slagle-Webb, A. B. Madhankumar, X. Liu, M. Klinger, I. A. Simpson, and J. R. Connor Ferritin: a novel mechanism for delivery of iron to the brain and other organs Am J Physiol Cell Physiol, August 1, 2007; 293(2): C641 - C649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Li, C. Luo, M. Mines, J. Zhang, and G.-H. Fan Chemokine CXCL12 Induces Binding of Ferritin Heavy Chain to the Chemokine Receptor CXCR4, Alters CXCR4 Signaling, and Induces Phosphorylation and Nuclear Translocation of Ferritin Heavy Chain J. Biol. Chem., December 8, 2006; 281(49): 37616 - 37627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Iwasaki, E. L. MacKenzie, K. Hailemariam, K. Sakamoto, and Y. Tsuji Hemin-Mediated Regulation of an Antioxidant-Responsive Element of the Human Ferritin H Gene and Role of Ref-1 during Erythroid Differentiation of K562 Cells. Mol. Cell. Biol., April 1, 2006; 26(7): 2845 - 2856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Every, J. P. Leader, A. C. B. Molteno, T. H. Bevin, and G. Sanderson Ultraviolet Photography of the In Vivo Human Cornea Unmasks the Hudson-Stahli Line and Physiologic Vortex Patterns Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3616 - 3622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pappa, D. Brown, Y. Koutalos, J. DeGregori, C. White, and V. Vasiliou Human Aldehyde Dehydrogenase 3A1 Inhibits Proliferation and Promotes Survival of Human Corneal Epithelial Cells J. Biol. Chem., July 29, 2005; 280(30): 27998 - 28006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Surguladze, K. M. Thompson, J. L. Beard, J. R. Connor, and M. G. Fried Interactions and Reactions of Ferritin with DNA J. Biol. Chem., April 9, 2004; 279(15): 14694 - 14702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Millholland, J. M. Fitch, C. X. Cai, E. P. Gibney, K. E. Beazley, and T. F. Linsenmayer Ferritoid, a Tissue-specific Nuclear Transport Protein for Ferritin in Corneal Epithelial Cells J. Biol. Chem., June 20, 2003; 278(26): 23963 - 23970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Thompson, M. G. Fried, Z. Ye, P. Boyer, and J. R. Connor Regulation, mechanisms and proposed function of ferritin translocation to cell nuclei J. Cell Sci., May 15, 2002; 115(10): 2165 - 2177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Torti and S. V. Torti Regulation of ferritin genes and protein Blood, May 15, 2002; 99(10): 3505 - 3516. [Full Text] [PDF]
|
|
|
|
|
|
C. X. Cai and T. F. Linsenmayer Nuclear translocation of ferritin in corneal epithelial cells J. Cell Sci., March 8, 2002; 114(12): 2327 - 2334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yang and F. Carrier The UV-inducible RNA-binding Protein A18 (A18 hnRNP) Plays a Protective Role in the Genotoxic Stress Response J. Biol. Chem., December 7, 2001; 276(50): 47277 - 47284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Maurer, A. Molai, R. D. Parker, Li Li, G. J. Carr, W. M. Petroll, H. D. Cavanagh, and J. V. Jester Pathology of Ocular Irritation with Bleaching Agents in the Rabbit Low-Volume Eye Test Toxicol Pathol, April 1, 2001; 29(3): 308 - 319. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Schultze-Mosgau, A. C. Katzur, K. K. Arora, S. S. Stojilkovic, K. Diedrich, and O. Ortmann Characterization of calcium-mobilizing, purinergic P2Y2 receptors in human ovarian cancer cells Mol. Hum. Reprod., May 1, 2000; 6(5): 435 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-Y. Yeh, M. Yeh, and J. Glass Glucocorticoids and dietary iron regulate postnatal intestinal heavy and light ferritin expression in rats Am J Physiol Gastrointest Liver Physiol, February 1, 2000; 278(2): G217 - G226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sun, T.-T. Sun, and R. M. Lavker Identification of a Cytosolic NADP+-dependent Isocitrate Dehydrogenase That Is Preferentially Expressed in Bovine Corneal Epithelium. A CORNEAL EPITHELIAL CRYSTALLIN J. Biol. Chem., June 11, 1999; 274(24): 17334 - 17341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Pountney, G Trugnan, M Bourgeois, and C Beaumont The identification of ferritin in the nucleus of K562 cells, and investigation of a possible role in the transcriptional regulation of adult beta-globin gene expression J. Cell Sci., January 3, 1999; 112(6): 825 - 831. [Abstract] [PDF]
|
|
|
|
|
|
A. Lachowicz, F. Van Goor, A. C. Katzur, G. Bonhomme, and S. S. Stojilkovic Uncoupling of Calcium Mobilization and Entry Pathways in Endothelin-stimulated Pituitary Lactotrophs J. Biol. Chem., November 7, 1997; 272(45): 28308 - 28314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zheng, L. Z. Krsmanovic, L. A. Vergara, K. J. Catt, and S. S. Stojilkovic Dependence of intracellular signaling and neurosecretion on phospholipase D activation in immortalized gonadotropin-releasing hormone neurons PNAS, February 18, 1997; 94(4): 1573 - 1578. [Abstract] [Full Text] [PDF]
|
|
