![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 5, 1221-1233, May 1998
and
Department of Molecular Biology and Biochemistry, Osaka University
Medical School, Suita 565-0871, Japan
Rho1p is a yeast homolog of mammalian RhoA small GTP-binding
protein. Rho1p is localized at the growth sites and required for bud
formation. We have recently shown that Bni1p is a potential target of
Rho1p and that Bni1p regulates reorganization of the actin cytoskeleton
through interactions with profilin, an actin monomer-binding protein.
Using the yeast two-hybrid screening system, we cloned a gene encoding
a protein that interacted with Bni1p. This protein, Spa2p, was known to
be localized at the bud tip and to be implicated in the establishment
of cell polarity. The C-terminal 254 amino acid region of Spa2p,
Spa2p(1213-1466), directly bound to a 162-amino acid region of Bni1p,
Bni1p(826-987). Genetic analyses revealed that both the
bni1 and spa2 mutations showed synthetic
lethal interactions with mutations in the genes encoding components of
the Pkc1p-mitogen-activated protein kinase pathway, in which Pkc1p is
another target of Rho1p. Immunofluorescence microscopic analysis showed
that Bni1p was localized at the bud tip in wild-type cells. However, in
the spa2 mutant, Bni1p was not localized at the bud tip
and instead localized diffusely in the cytoplasm. A mutant Bni1p, which
lacked the Rho1p-binding region, also failed to be localized at the bud
tip. These results indicate that both Rho1p and Spa2p are involved in
the localization of Bni1p at the growth sites where Rho1p regulates
reorganization of the actin cytoskeleton through Bni1p.
This article has been cited by other articles:
|
|
 |
|
  L. Gao and A. Bretscher Analysis of Unregulated Formin Activity Reveals How Yeast Can Balance F-Actin Assembly between Different Microfilament-based Organizations Mol. Biol. Cell, April 1, 2008; 19(4): 1474 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Juanes, E. Queralt, M. C. Bano, and J. C. Igual Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycle J. Cell Sci., July 15, 2007; 120(14): 2390 - 2401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Buttery, S. Yoshida, and D. Pellman Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables Mol. Biol. Cell, May 1, 2007; 18(5): 1826 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T. Bettinger, M. G. Clark, and D. C. Amberg Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin Recovery From Osmotic Stress in Saccharomyces cerevisiae Genetics, April 1, 2007; 175(4): 1637 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Steinberg Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper Eukaryot. Cell, March 1, 2007; 6(3): 351 - 360. [Full Text] [PDF]
|
|
|
|
|
|
P. Knechtle, J. Wendland, and P. Philippsen The SH3/PH Domain Protein AgBoi1/2 Collaborates with the Rho-Type GTPase AgRho3 To Prevent Nonpolar Growth at Hyphal Tips of Ashbya gossypii Eukaryot. Cell, October 1, 2006; 5(10): 1635 - 1647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C.G. Rida, A. Nishikawa, G. Y. Won, and N. Dean Yeast-to-Hyphal Transition Triggers Formin-dependent Golgi Localization to the Growing Tip in Candida albicans Mol. Biol. Cell, October 1, 2006; 17(10): 4364 - 4378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Moseley and B. L. Goode The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 605 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Rodicio, S. Koch, H.-P. Schmitz, and J. J. Heinisch KlRHO1 and KlPKC1 are essential for cell integrity signalling in Kluyveromyces lactis. Microbiology, September 1, 2006; 152(Pt 9): 2635 - 2649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Tcheperegine, X.-D. Gao, and E. Bi Regulation of Cell Polarity by Interactions of Msb3 and Msb4 with Cdc42 and Polarisome Components Mol. Cell. Biol., October 1, 2005; 25(19): 8567 - 8580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Martin, A. Walther, and J. Wendland Ras1-Induced Hyphal Development in Candida albicans Requires the Formin Bni1 Eukaryot. Cell, October 1, 2005; 4(10): 1712 - 1724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Shih, S. L. Reck-Peterson, R. Newitt, M. S. Mooseker, R. Aebersold, and I. Herskowitz Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae Mol. Biol. Cell, October 1, 2005; 16(10): 4595 - 4608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Noma, K. Iida, and H. Iida Polarized Morphogenesis Regulator Spa2 Is Required for the Function of Putative Stretch-Activated Ca2+-Permeable Channel Component Mid1 in Saccharomyces cerevisiae Eukaryot. Cell, August 1, 2005; 4(8): 1353 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Li, Y. M. Wang, X. De Zheng, H. Y. Liang, J. C. W. Tang, and Y. Wang The formin family protein CaBni1p has a role in cell polarity control during both yeast and hyphal growth in Candida albicans J. Cell Sci., June 15, 2005; 118(12): 2637 - 2648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Levin Cell Wall Integrity Signaling in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 262 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Boyd, T. Hughes, M. Pypaert, and P. Novick Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p J. Cell Biol., December 6, 2004; 167(5): 889 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kadota, T. Yamamoto, S. Yoshiuchi, E. Bi, and K. Tanaka Septin Ring Assembly Requires Concerted Action of Polarisome Components, a PAK Kinase Cla4p, and the Actin Cytoskeleton in Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2004; 15(12): 5329 - 5345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pruyne, L. Gao, E. Bi, and A. Bretscher Stable and Dynamic Axes of Polarity Use Distinct Formin Isoforms in Budding Yeast Mol. Biol. Cell, November 1, 2004; 15(11): 4971 - 4989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Matheos, M. Metodiev, E. Muller, D. Stone, and M. D. Rose Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p J. Cell Biol., April 12, 2004; 165(1): 99 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Mackin, T. J. Sousou, and S. E. Erdman The PXL1 Gene of Saccharomyces cerevisiae Encodes a Paxillin-like Protein Functioning in Polarized Cell Growth Mol. Biol. Cell, April 1, 2004; 15(4): 1904 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Moseley, I. Sagot, A. L. Manning, Y. Xu, M. J. Eck, D. Pellman, and B. L. Goode A Conserved Mechanism for Bni1- and mDia1-induced Actin Assembly and Dual Regulation of Bni1 by Bud6 and Profilin Mol. Biol. Cell, February 1, 2004; 15(2): 896 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bidlingmaier and M. Snyder Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators J. Cell Biol., January 19, 2004; 164(2): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Nelson, A. B. Parsons, M. Evangelista, K. Schaefer, K. Kennedy, S. Ritchie, T. L. Petryshen, and C. Boone Fus1p Interacts With Components of the Hog1p Mitogen-Activated Protein Kinase and Cdc42p Morphogenesis Signaling Pathways to Control Cell Fusion During Yeast Mating Genetics, January 1, 2004; 166(1): 67 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Huang, G. Zeng, A. Y.J. Ng, and M. Cai Identification of Novel Recognition Motifs and Regulatory Targets for the Yeast Actin-regulating Kinase Prk1p Mol. Biol. Cell, December 1, 2003; 14(12): 4871 - 4884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Muller, N. A. Mackin, S. E. Erdman, and K. W. Cunningham Fig1p Facilitates Ca2+ Influx and Cell Fusion during Mating of Saccharomyces cerevisiae J. Biol. Chem., October 3, 2003; 278(40): 38461 - 38469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Knechtle, F. Dietrich, and P. Philippsen Maximal Polar Growth Potential Depends on the Polarisome Component AgSpa2 in the Filamentous Fungus Ashbya gossypii Mol. Biol. Cell, October 1, 2003; 14(10): 4140 - 4154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yuzyuk and D. C. Amberg Actin Recovery and Bud Emergence in Osmotically Stressed Cells Requires the Conserved Actin Interacting Mitogen-activated Protein Kinase Kinase Kinase Ssk2p/MTK1 and the Scaffold Protein Spa2p Mol. Biol. Cell, July 1, 2003; 14(7): 3013 - 3026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Dong, D. Pruyne, and A. Bretscher Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast J. Cell Biol., June 23, 2003; 161(6): 1081 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Goehring, D. A. Mitchell, A. H. Y. Tong, M. E. Keniry, C. Boone, and G. F. Sprague Jr. Synthetic Lethal Analysis Implicates Ste20p, a p21-activated Protein Kinase, in Polarisome Activation Mol. Biol. Cell, April 1, 2003; 14(4): 1501 - 1516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bretscher Polarized growth and organelle segregation in yeast: the tracks, motors, and receptors J. Cell Biol., March 17, 2003; 160(6): 811 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Cullen and G. F. Sprague Jr. The Glc7p-Interacting Protein Bud14p Attenuates Polarized Growth, Pheromone Response, and Filamentous Growth in Saccharomyces cerevisiae Eukaryot. Cell, December 1, 2002; 1(6): 884 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Cullen and G. F. Sprague Jr. The Roles of Bud-Site-Selection Proteins during Haploid Invasive Growth in Yeast Mol. Biol. Cell, September 1, 2002; 13(9): 2990 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hohmann Osmotic Stress Signaling and Osmoadaptation in Yeasts Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 300 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mochida, T. Yamamoto, K. Fujimura-Kamada, and K. Tanaka The Novel Adaptor Protein, Mti1p, and Vrp1p, a Homolog of Wiskott-Aldrich Syndrome Protein-Interacting Protein (WIP), May Antagonistically Regulate Type I Myosins in Saccharomyces cerevisiae Genetics, March 1, 2002; 160(3): 923 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-P. Schmitz, S. Huppert, A. Lorberg, and J. J. Heinisch Rho5p downregulates the yeast cell integrity pathway J. Cell Sci., January 8, 2002; 115(15): 3139 - 3148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kawabe, H. Nakanishi, M. Asada, A. Fukuhara, K. Morimoto, M. Takeuchi, and Y. Takai Pilt, a Novel Peripheral Membrane Protein at Tight Junctions in Epithelial Cells J. Biol. Chem., December 14, 2001; 276(51): 48350 - 48355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Drees, B. Sundin, E. Brazeau, J. P. Caviston, G.-C. Chen, W. Guo, K. G. Kozminski, M. W. Lau, J. J. Moskow, A. Tong, et al. A protein interaction map for cell polarity development J. Cell Biol., August 6, 2001; 154(3): 549 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Harkins, N. Page, L. R. Schenkman, C. De Virgilio, S. Shaw, H. Bussey, and J. R. Pringle Bud8p and Bud9p, Proteins That May Mark the Sites for Bipolar Budding in Yeast Mol. Biol. Cell, August 1, 2001; 12(8): 2497 - 2518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ni and M. Snyder A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2001; 12(7): 2147 - 2170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ozaki-Kuroda, Y. Yamamoto, H. Nohara, M. Kinoshita, T. Fujiwara, K. Irie, and Y. Takai Dynamic Localization and Function of Bni1p at the Sites of Directed Growth in Saccharomyces cerevisiae Mol. Cell. Biol., February 1, 2001; 21(3): 827 - 839. [Abstract] [Full Text]
|
|
|
|
|
|
H.-U. Mösch, T. Köhler, and G. H. Braus Different Domains of the Essential GTPase Cdc42p Required for Growth and Development of Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2001; 21(1): 235 - 248. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Takai, T. Sasaki, and T. Matozaki Small GTP-Binding Proteins Physiol Rev, January 1, 2001; 81(1): 153 - 208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Segal, K. Bloom, and S. I. Reed Bud6 Directs Sequential Microtubule Interactions with the Bud Tip and Bud Neck during Spindle Morphogenesis in Saccharomyces cerevisiae Mol. Biol. Cell, November 1, 2000; 11(11): 3689 - 3702. [Abstract] [Full Text]
|
|
|
|
|
|
M. Jaquenoud and M. Peter Gic2p May Link Activated Cdc42p to Components Involved in Actin Polarization, Including Bni1p and Bud6p (Aip3p) Mol. Cell. Biol., September 1, 2000; 20(17): 6244 - 6258. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-J. Sheu, Y. Barral, and M. Snyder Polarized Growth Controls Cell Shape and Bipolar Bud Site Selection in Saccharomyces cerevisiae Mol. Cell. Biol., July 15, 2000; 20(14): 5235 - 5247. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. J. Roberts, B. Nelson, M. J. Marton, R. Stoughton, M. R. Meyer, H. A. Bennett, Y. D. He, H. Dai, W. L. Walker, T. R. Hughes, et al. Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles Science, February 4, 2000; 287(5454): 873 - 880. [Abstract] [Full Text]
|
|
|
|
|
|
E. A. Vallen, J. Caviston, and E. Bi Roles of Hof1p, Bni1p, Bnr1p, and Myo1p in Cytokinesis in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2000; 11(2): 593 - 611. [Abstract] [Full Text]
|
|
|
|
|
|
E. Bi, J. B. Chiavetta, H. Chen, G.-C. Chen, C. S. M. Chan, and J. R. Pringle Identification of Novel, Evolutionarily Conserved Cdc42p-interacting Proteins and of Redundant Pathways Linking Cdc24p and Cdc42p to Actin Polarization in Yeast Mol. Biol. Cell, February 1, 2000; 11(2): 773 - 793. [Abstract] [Full Text]
|
|
|
|
|
|
T. Fujiwara, K. Tanaka, E. Inoue, M. Kikyo, and Y. Takai Bni1p Regulates Microtubule-Dependent Nuclear Migration through the Actin Cytoskeleton in Saccharomyces cerevisiae Mol. Cell. Biol., December 1, 1999; 19(12): 8016 - 8027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakano, K. Takaishi, A. Kodama, A. Mammoto, H. Shiozaki, M. Monden, and Y. Takai Distinct Actions and Cooperative Roles of ROCK and mDia in Rho Small G Protein-induced Reorganization of the Actin Cytoskeleton in Madin-Darby Canine Kidney Cells Mol. Biol. Cell, August 1, 1999; 10(8): 2481 - 2491. [Abstract] [Full Text]
|
|
|
|
|
|
L. Lee, S. K. Klee, M. Evangelista, C. Boone, and D. Pellman Control of Mitotic Spindle Position by the Saccharomyces cerevisiae Formin Bni1p J. Cell Biol., March 8, 1999; 144(5): 947 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Miller, D. Matheos, and M. D. Rose The Cortical Localization of the Microtubule Orientation Protein, Kar9p, Is Dependent upon Actin and Proteins Required for Polarization J. Cell Biol., March 8, 1999; 144(5): 963 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Johnson Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 54 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamei, K. Tanaka, T. Hihara, M. Umikawa, H. Imamura, M. Kikyo, K. Ozaki, and Y. Takai Interaction of Bnr1p with a Novel Src Homology 3 Domain-containing Hof1p. IMPLICATION IN CYTOKINESIS IN SACCHAROMYCES CEREVISIAE J. Biol. Chem., October 23, 1998; 273(43): 28341 - 28345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Sharpless and S. D. Harris Functional Characterization and Localization of the Aspergillus nidulans Formin SEPA Mol. Biol. Cell, February 1, 2002; 13(2): 469 - 479. [Abstract] [Full Text] [PDF]
|
|
