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Vol. 9, Issue 5, 977-991, May 1998
and
*Lehrstuhl für Angewandte Mikrobiologie, Biozentrum,
Universität Basel, CH-4056 Basel, Switzerland; and
Cnm67p, a novel yeast protein, localizes to the microtubule
organizing center, the spindle pole body (SPB). Deletion of
CNM67 (YNL225c) frequently results in spindle
misorientation and impaired nuclear migration, leading to the
generation of bi- and multinucleated cells (40%). Electron microscopy
indicated that CNM67 is required for proper formation of the
SPB outer plaque, a structure that nucleates cytoplasmic (astral)
microtubules. Interestingly, cytoplasmic microtubules that are
essential for spindle orientation and nuclear migration are still
present in cnm67Medical Research Council Laboratory of Molecular Biology,
Cambridge CB2 2QH, United Kingdom
1 cells that lack a detectable outer
plaque. These microtubules are attached to the SPB half- bridge
throughout the cell cycle. This interaction presumably allows for
low-efficiency nuclear migration and thus provides a rescue mechanism
in the absence of a functional outer plaque. Although
CNM67 is not strictly required for mitosis, it is
essential for sporulation. Time-lapse microscopy of
cnm671 cells with green fluorescent protein
(GFP)-labeled nuclei indicated that CNM67 is dispensable
for nuclear migration (congression) and nuclear fusion during
conjugation. This is in agreement with previous data, indicating that
cytoplasmic microtubules are organized by the half-bridge during
mating.
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