![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 6, 1253-1263, June 1998
Laboratoire de Physiologie Cellulaire et de Génétique
des Levures, Université Libre de Bruxelles-Campus Plaine CP244,
B-1050 Brussels, Belgium
Addition of ammonium ions to yeast cells growing on proline as the
sole nitrogen source induces rapid inactivation and degradation of the
general amino acid permease Gap1 through a process requiring the
Npi1/Rsp5 ubiquitin (Ub) ligase. In this study, we show that NH4+ induces endocytosis of Gap1, which is then
delivered into the vacuole where it is degraded. This down-regulation
is accompanied by increased conversion of Gap1 to ubiquitinated forms.
Ubiquitination and subsequent degradation of Gap1 are impaired in the
npi1 strain. In this mutant, the amount of Npi1/Rsp5 Ub
ligase is reduced >10-fold compared with wild-type cells. The
C-terminal tail of Gap1 contains sequences, including a di-leucine
motif, which are required for NH4+-induced
internalization and degradation of the permease. We show here that
mutant Gap1 permeases affected in these sequences still bind Ub.
Furthermore, we provide evidence that only a small fraction of Gap1 is
modified by Ub after addition of NH4+ to
mutants defective in endocytosis.
This article has been cited by other articles:
|
|
 |
|
  A. Herrador, S. Herranz, D. Lara, and O. Vincent Recruitment of the ESCRT Machinery to a Putative Seven-Transmembrane-Domain Receptor Is Mediated by an Arrestin-Related Protein Mol. Cell. Biol., February 15, 2010; 30(4): 897 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lauwers, C. Jacob, and B. Andre K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway J. Cell Biol., May 4, 2009; 185(3): 493 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Pokrzywa, B. Guerriat, J. Dodzian, and P. Morsomme Dual Sorting of the Saccharomyces cerevisiae Vacuolar Protein Sna4p Eukaryot. Cell, March 1, 2009; 8(3): 278 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Wiederhold, T. Gandhi, H. P. Permentier, R. Breitling, B. Poolman, and D. J. Slotboom The Yeast Vacuolar Membrane Proteome Mol. Cell. Proteomics, February 1, 2009; 8(2): 380 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Adle and J. Lee Expressional Control of a Cadmium-transporting P1B-type ATPase by a Metal Sensing Degradation Signal J. Biol. Chem., November 14, 2008; 283(46): 31460 - 31468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Haitani and H. Takagi Rsp5 is required for the nuclear export of mRNA of HSF1 and MSN2/4 under stress conditions in Saccharomyces cerevisiae. Genes Cells, February 1, 2008; 13(2): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Calcagno-Pizarelli, S. Negrete-Urtasun, S. H. Denison, J. D. Rudnicka, H.-J. Bussink, T. Munera-Huertas, L. Stanton, A. Hervas-Aguilar, E. A. Espeso, J. Tilburn, et al. Establishment of the Ambient pH Signaling Complex in Aspergillus nidulans: PalI Assists Plasma Membrane Localization of PalH Eukaryot. Cell, December 1, 2007; 6(12): 2365 - 2375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nikko and B. Andre Split-Ubiquitin Two-Hybrid Assay To Analyze Protein-Protein Interactions at the Endosome: Application to Saccharomyces cerevisiae Bro1 Interacting with ESCRT Complexes, the Doa4 Ubiquitin Hydrolase, and the Rsp5 Ubiquitin Ligase Eukaryot. Cell, August 1, 2007; 6(8): 1266 - 1277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Lauwers, G. Grossmann, and B. Andre Evidence for Coupled Biogenesis of Yeast Gap1 Permease and Sphingolipids: Essential Role in Transport Activity and Normal Control by Ubiquitination Mol. Biol. Cell, August 1, 2007; 18(8): 3068 - 3080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Sullivan, M. J. Lewis, E. Nikko, and H. R.B. Pelham Multiple Interactions Drive Adaptor-Mediated Recruitment of the Ubiquitin Ligase Rsp5 to Membrane Proteins In Vivo and In Vitro Mol. Biol. Cell, July 1, 2007; 18(7): 2429 - 2440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miranda and A. Sorkin Regulation of Receptors and Transporters by Ubiquitination: New Insights into Surprisingly Similar Mechanisms Mol. Interv., June 1, 2007; 7(3): 157 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kota, C. F. Gilstring, and P. O. Ljungdahl Membrane chaperone Shr3 assists in folding amino acid permeases preventing precocious ERAD J. Cell Biol., February 26, 2007; 176(5): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Feller, M. Boeckstaens, A. M. Marini, and E. Dubois Transduction of the Nitrogen Signal Activating Gln3-mediated Transcription Is Independent of Npr1 Kinase and Rsp5-Bul1/2 Ubiquitin Ligase in Saccharomyces cerevisiae J. Biol. Chem., September 29, 2006; 281(39): 28546 - 28554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Tate, R. Rai, and T. G. Cooper Ammonia-specific Regulation of Gln3 Localization in Saccharomyces cerevisiae by Protein Kinase Npr1 J. Biol. Chem., September 22, 2006; 281(38): 28460 - 28469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Navarro, F. Machin, Y. Martin, and J. M. Siverio Down-regulation of Eukaryotic Nitrate Transporter by Nitrogen-dependent Ubiquitinylation J. Biol. Chem., May 12, 2006; 281(19): 13268 - 13274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub and D. Rotin Role of Ubiquitylation in Cellular Membrane Transport Physiol Rev, April 1, 2006; 86(2): 669 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Peter, L. During, and A. Ahmed Carbon Catabolite Repression Regulates Amino Acid Permeases in Saccharomyces cerevisiae via the TOR Signaling Pathway J. Biol. Chem., March 3, 2006; 281(9): 5546 - 5552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Felice, I. De Domenico, L. Li, D. M. Ward, B. Bartok, G. Musci, and J. Kaplan Post-transcriptional Regulation of the Yeast High Affinity Iron Transport System J. Biol. Chem., June 10, 2005; 280(23): 22181 - 22190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wiedemuth and K. D. Breunig Role of Snf1p in Regulation of Intracellular Sorting of the Lactose and Galactose Transporter Lac12p in Kluyveromyces lactis Eukaryot. Cell, April 1, 2005; 4(4): 716 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Su, W. B. Frommer, and U. Ludewig Molecular and Functional Characterization of a Family of Amino Acid Transporters from Arabidopsis Plant Physiology, October 1, 2004; 136(2): 3104 - 3113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Crespo, S. B. Helliwell, C. Wiederkehr, P. Demougin, B. Fowler, M. Primig, and M. N. Hall NPR1 Kinase and RSP5-BUL1/2 Ubiquitin Ligase Control GLN3-dependent Transcription in Saccharomyces cerevisiae J. Biol. Chem., September 3, 2004; 279(36): 37512 - 37517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Subramanian, C. A. Woolford, and E. W. Jones The Sec1/Munc18 Protein, Vps33p, Functions at the Endosome and the Vacuole of Saccharomyces cerevisiae Mol. Biol. Cell, June 1, 2004; 15(6): 2593 - 2605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-O. Blondel, J. Morvan, S. Dupre, D. Urban-Grimal, R. Haguenauer-Tsapis, and C. Volland Direct Sorting of the Yeast Uracil Permease to the Endosomal System Is Controlled by Uracil Binding and Rsp5p-dependent Ubiquitylation Mol. Biol. Cell, February 1, 2004; 15(2): 883 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Polo, S. Confalonieri, A. E. Salcini, and P. P. Di Fiore EH and UIM: Endocytosis and More Sci. Signal., December 16, 2003; 2003(213): re17 - re17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nikko, A.-M. Marini, and B. Andre Permease Recycling and Ubiquitination Status Reveal a Particular Role for Bro1 in the Multivesicular Body Pathway J. Biol. Chem., December 12, 2003; 278(50): 50732 - 50743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Abe and H. Iida Pressure-Induced Differential Regulation of the Two Tryptophan Permeases Tat1 and Tat2 by Ubiquitin Ligase Rsp5 and Its Binding Proteins, Bul1 and Bul2 Mol. Cell. Biol., November 1, 2003; 23(21): 7566 - 7584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hoshikawa, M. Shichiri, S. Nakamori, and H. Takagi A nonconserved Ala401 in the yeast Rsp5 ubiquitin ligase is involved in degradation of Gap1 permease and stress-induced abnormal proteins PNAS, September 30, 2003; 100(20): 11505 - 11510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Biswas, M. Roy, and A. Datta N-Acetylglucosamine-inducible CaGAP1 encodes a general amino acid permease which co-ordinates external nitrogen source response and morphogenesis in Candida albicans Microbiology, September 1, 2003; 149(9): 2597 - 2608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Boase, R. A. Lockington, J. R. J. Adams, L. Rodbourn, and J. M. Kelly Molecular Characterization and Analysis of the acrB Gene of Aspergillus nidulans: A Gene Identified by Genetic Interaction As a Component of the Regulatory Network That Includes the CreB Deubiquitination Enzyme Genetics, May 1, 2003; 164(1): 95 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-O. De Craene, O. Soetens, and B. Andre The Npr1 Kinase Controls Biosynthetic and Endocytic Sorting of the Yeast Gap1 Permease J. Biol. Chem., November 16, 2001; 276(47): 43939 - 43948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Soetens, J.-O. De Craene, and B. Andre Ubiquitin Is Required for Sorting to the Vacuole of the Yeast General Amino Acid Permease, Gap1 J. Biol. Chem., November 16, 2001; 276(47): 43949 - 43957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Forsberg, M. Hammar, C. Andreasson, A. Moliner, and P. O. Ljungdahl Suppressors of ssy1 and ptr3 Null Mutations Define Novel Amino Acid Sensor-Independent Genes in Saccharomyces cerevisiae Genetics, July 1, 2001; 158(3): 973 - 988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Horak and D. H. Wolf Glucose-Induced Monoubiquitination of the Saccharomyces cerevisiae Galactose Transporter Is Sufficient To Signal Its Internalization J. Bacteriol., May 15, 2001; 183(10): 3083 - 3088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wang, J. M. McCaffery, B. Wendland, S. Dupre, R. Haguenauer-Tsapis, and J. M. Huibregtse Localization of the Rsp5p Ubiquitin-Protein Ligase at Multiple Sites within the Endocytic Pathway Mol. Cell. Biol., May 15, 2001; 21(10): 3564 - 3575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Helliwell, S. Losko, and C. A. Kaiser Components of a Ubiquitin Ligase Complex Specify Polyubiquitination and Intracellular Trafficking of the General Amino Acid Permease J. Cell Biol., May 14, 2001; 153(4): 649 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Johnston, S. Enomoto, L. Schneper, M. C. McClellan, F. Twu, N. D. Montgomery, S. A. Haney, J. R. Broach, and J. Berman CAC3 (MSI1) Suppression of RAS2G19V Is Independent of Chromatin Assembly Factor I and Mediated by NPR1 Mol. Cell. Biol., March 1, 2001; 21(5): 1784 - 1794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Forsberg and P. O. Ljungdahl Genetic and Biochemical Analysis of the Yeast Plasma Membrane Ssy1p-Ptr3p-Ssy5p Sensor of Extracellular Amino Acids Mol. Cell. Biol., February 1, 2001; 21(3): 814 - 826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dunn and L. Hicke Domains of the Rsp5 Ubiquitin-Protein Ligase Required for Receptor-mediated and Fluid-Phase Endocytosis Mol. Biol. Cell, February 1, 2001; 12(2): 421 - 435. [Abstract] [Full Text]
|
|
|
|
|
|
B. Gajewska, J. Kaminska, A. Jesionowska, N. C. Martin, A. K. Hopper, and T. Zoladek WW Domains of Rsp5p Define Different Functions: Determination of Roles in Fluid Phase and Uracil Permease Endocytosis in Saccharomyces cerevisiae Genetics, January 1, 2001; 157(1): 91 - 101. [Abstract] [Full Text]
|
|
|
|
|
|
F. Abe and K. Horikoshi Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae Mol. Cell. Biol., November 1, 2000; 20(21): 8093 - 8102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Andoh, Y. Hirata, and A. Kikuchi Yeast Glycogen Synthase Kinase 3 Is Involved in Protein Degradation in Cooperation with Bul1, Bul2, and Rsp5 Mol. Cell. Biol., September 15, 2000; 20(18): 6712 - 6720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Srivastava, C. A. Woolford, and E. W. Jones Pep3p/Pep5p Complex: A Putative Docking Factor at Multiple Steps of Vesicular Transport to the Vacuole of Saccharomyces cerevisiae Genetics, September 1, 2000; 156(1): 105 - 122. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. Plant, F. Lafont, S. Lecat, P. Verkade, K. Simons, and D. Rotin Apical Membrane Targeting of Nedd4 Is Mediated by an Association of Its C2 Domain with Annexin Xiiib J. Cell Biol., June 26, 2000; 149(7): 1473 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wiederkehr, S. Avaro, C. Prescianotto-Baschong, R. Haguenauer-Tsapis, and H. Riezman The F-Box Protein Rcy1p Is Involved in Endocytic Membrane Traffic and Recycling Out of an Early Endosome in Saccharomyces cerevisiae J. Cell Biol., April 17, 2000; 149(2): 397 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Gilstring, M. Melin-Larsson, and P. O. Ljungdahl Shr3p Mediates Specific COPII Coatomer-Cargo Interactions Required for the Packaging of Amino Acid Permeases Into ER-derived Transport Vesicles Mol. Biol. Cell, November 1, 1999; 10(11): 3549 - 3565. [Abstract] [Full Text]
|
|
|
|
|
|
T. Beck, A. Schmidt, and M. N. Hall Starvation Induces Vacuolar Targeting and Degradation of the Tryptophan Permease in Yeast J. Cell Biol., September 20, 1999; 146(6): 1227 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Fisk and M. P. Yaffe A Role for Ubiquitination in Mitochondrial Inheritance in Saccharomyces cerevisiae J. Cell Biol., June 14, 1999; 145(6): 1199 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Iraqui, S. Vissers, B. Andre, and A. Urrestarazu Transcriptional Induction by Aromatic Amino Acids in Saccharomyces cerevisiae Mol. Cell. Biol., May 1, 1999; 19(5): 3360 - 3371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Seron, M.-O. Blondel, R. Haguenauer-Tsapis, and C. Volland Uracil-Induced Down-Regulation of the Yeast Uracil Permease J. Bacteriol., March 15, 1999; 181(6): 1793 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Springael, J. Galan, R Haguenauer-Tsapis, and B Andre NH4+-induced down-regulation of the Saccharomyces cerevisiae Gap1p permease involves its ubiquitination with lysine-63-linked chains J. Cell Sci., January 5, 1999; 112(9): 1375 - 1383. [Abstract] [PDF]
|
|
|
|
|
|
G. Wang, J. Yang, and J. M. Huibregtse Functional Domains of the Rsp5 Ubiquitin-Protein Ligase Mol. Cell. Biol., January 1, 1999; 19(1): 342 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Medintz, H. Jiang, and C. A. Michels The Role of Ubiquitin Conjugation in Glucose-induced Proteolysis of Saccharomyces Maltose Permease J. Biol. Chem., December 18, 1998; 273(51): 34454 - 34462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chang, S. Cheang, X. Espanel, and M. Sudol Rsp5 WW Domains Interact Directly with the Carboxyl-terminal Domain of RNA Polymerase II J. Biol. Chem., June 30, 2000; 275(27): 20562 - 20571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Graschopf, J. A. Stadler, M. K. Hoellerer, S. Eder, M. Sieghardt, S. D. Kohlwein, and R. J. Schweyen The Yeast Plasma Membrane Protein Alr1 Controls Mg2+ Homeostasis and Is Subject to Mg2+-dependent Control of Its Synthesis and Degradation J. Biol. Chem., May 4, 2001; 276(19): 16216 - 16222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Hofmann and C. M. Pickart In Vitro Assembly and Recognition of Lys-63 Polyubiquitin Chains J. Biol. Chem., July 20, 2001; 276(30): 27936 - 27943. [Abstract] [Full Text] [PDF]
|
|
