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Vol. 9, Issue 6, 1293-1308, June 1998
-Tubulin, a New
Member of the Tubulin Superfamily
Department of Molecular, Cellular, and Developmental Biology,
University of Colorado, Boulder, Boulder, Colorado 80309-0347
We have cloned the UNI3 gene in
Chlamydomonas and find that it encodes a new member of
the tubulin superfamily. Although Uni3p shares significant sequence
identity with 
-, 
-, and 
-tubulins, there is a region of Uni3p
that has no similarity to tubulins or other known proteins. Mutant
uni3-1 cells assemble zero, one, or two flagella.
Pedigree analysis suggests that flagellar number in
uni3-1 cells is a function of the age of the cell. The
uniflagellate uni3-1 cells show a positional phenotype;
the basal body opposite the eyespot templates the single flagellum. A
percentage of uni3-1 cells also fail to orient the
cleavage furrow properly, and basal bodies have been implicated in the
placement of cleavage furrows in Chlamydomonas. Finally
when uni3-1 cells are observed by electron microscopy,
doublet rather than triplet microtubules are observed at the proximal
end of the basal bodies. We propose that the Uni3 tubulin is involved
in both the function and cell cycle-dependent maturation of basal
bodies/centrioles.
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