![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 6, 1395-1410, June 1998
Princeton University, Princeton, New Jersey 08544-1014
Cell fusion in yeast is the process by which two haploid cells fuse
to form a diploid zygote. To dissect the pathway of cell fusion, we
phenotypically and genetically characterized four cell fusion mutants,
fus6/spa2, fus7/rvs161, fus1, and fus2.
First, we examined the complete array of single and double mutants. In all cases but one, double mutants exhibited stronger cell fusion defects than single mutants. The exception was rvs161
fus2, suggesting that Rvs161p and Fus2p act in
concert. Dosage suppression analysis showed that Fus1p and Fus2p act
downstream or parallel to Rvs161p and Spa2p. Second, electron
microscopic analysis was used to define the mutant defects in cell
fusion. In wild-type prezygotes vesicles were aligned and clustered
across the cell fusion zone. The vesicles were associated with regions
of cell wall thinning. Analysis of Fus
zygotes indicated
that Fus1p was required for the normal localization of the vesicles to
the zone of cell fusion, and Spa2p facilitated their clustering. In
contrast, Fus2p and Rvs161p appeared to act after vesicle positioning.
These findings lead us to propose that cell fusion is mediated in part
by the localized release of vesicles containing components essential
for cell fusion.
This article has been cited by other articles:
|
|
 |
|
  J. M. Paterson, C. A. Ydenberg, and M. D. Rose Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating J. Cell Biol., May 19, 2008; 181(4): 697 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jin, J. M. McCaffery, and E. Grote Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast J. Cell Biol., February 25, 2008; 180(4): 813 - 826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Melloy, S. Shen, E. White, J. R. McIntosh, and M. D. Rose Nuclear fusion during yeast mating occurs by a three-step pathway J. Cell Biol., November 19, 2007; 179(4): 659 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Gattegno, A. Mittal, C. Valansi, K. C.Q. Nguyen, D. H. Hall, L. V. Chernomordik, and B. Podbilewicz Genetic Control of Fusion Pore Expansion in the Epidermis of Caenorhabditis elegans Mol. Biol. Cell, April 1, 2007; 18(4): 1153 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Shi, S. Kaminskyj, S. Caldwell, and M. C. Loewen A role for a complex between activated G protein-coupled receptors in yeast cellular mating PNAS, March 27, 2007; 104(13): 5395 - 5400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Aguilar, A. Engel, and P. Walter The Plasma Membrane Proteins Prm1 and Fig1 Ascertain Fidelity of Membrane Fusion during Yeast Mating Mol. Biol. Cell, February 1, 2007; 18(2): 547 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Barale, D. McCusker, and R. A. Arkowitz Cdc42p GDP/GTP Cycling Is Necessary for Efficient Cell Fusion during Yeast Mating Mol. Biol. Cell, June 1, 2006; 17(6): 2824 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nolan, A. E. Cowan, D. E. Koppel, H. Jin, and E. Grote FUS1 Regulates the Opening and Expansion of Fusion Pores between Mating Yeast Mol. Biol. Cell, May 1, 2006; 17(5): 2439 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ren, P. Vajjhala, J. S. Lee, B. Winsor, and A. L. Munn The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 37 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Shih, S. L. Reck-Peterson, R. Newitt, M. S. Mooseker, R. Aebersold, and I. Herskowitz Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae Mol. Biol. Cell, October 1, 2005; 16(10): 4595 - 4608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Derre and R. R. Isberg LidA, a Translocated Substrate of the Legionella pneumophila Type IV Secretion System, Interferes with the Early Secretory Pathway Infect. Immun., July 1, 2005; 73(7): 4370 - 4380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Fitch, A. E. Gammie, D. J. Lee, V. B. de Candal, and M. D. Rose Lrg1p Is a Rho1 GTPase-Activating Protein Required for Efficient Cell Fusion in Yeast Genetics, October 1, 2004; 168(2): 733 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Nichols, J. A. Fraser, and J. Heitman PAK Kinases Ste20 and Pak1 Govern Cell Polarity at Different Stages of Mating in Cryptococcus neoformans Mol. Biol. Cell, October 1, 2004; 15(10): 4476 - 4489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Barale, D. McCusker, and R. A. Arkowitz The Exchange Factor Cdc24 Is Required for Cell Fusion during Yeast Mating Eukaryot. Cell, August 1, 2004; 3(4): 1049 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Pearson, K. Xu, K. E. Sharpless, and S. D. Harris MesA, a Novel Fungal Protein Required for the Stabilization of Polarity Axes in Aspergillus nidulans Mol. Biol. Cell, August 1, 2004; 15(8): 3658 - 3672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Matheos, M. Metodiev, E. Muller, D. Stone, and M. D. Rose Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p J. Cell Biol., April 12, 2004; 165(1): 99 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Proszynski, K. Simons, and M. Bagnat O-Glycosylation as a Sorting Determinant for Cell Surface Delivery in Yeast Mol. Biol. Cell, April 1, 2004; 15(4): 1533 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bidlingmaier and M. Snyder Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators J. Cell Biol., January 19, 2004; 164(2): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Nelson, A. B. Parsons, M. Evangelista, K. Schaefer, K. Kennedy, S. Ritchie, T. L. Petryshen, and C. Boone Fus1p Interacts With Components of the Hog1p Mitogen-Activated Protein Kinase and Cdc42p Morphogenesis Signaling Pathways to Control Cell Fusion During Yeast Mating Genetics, January 1, 2004; 166(1): 67 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Muller, N. A. Mackin, S. E. Erdman, and K. W. Cunningham Fig1p Facilitates Ca2+ Influx and Cell Fusion during Mating of Saccharomyces cerevisiae J. Biol. Chem., October 3, 2003; 278(40): 38461 - 38469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Huang, M. Zhang, and S. E. Erdman Posttranslational Modifications Required for Cell Surface Localization and Function of the Fungal Adhesin Aga1p Eukaryot. Cell, October 1, 2003; 2(5): 1099 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Santos and M. Snyder Specific Protein Targeting during Cell Differentiation: Polarized Localization of Fus1p during Mating Depends on Chs5p in Saccharomyces cerevisiae Eukaryot. Cell, August 1, 2003; 2(4): 821 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhang, D. Bennett, and S. E. Erdman Maintenance of Mating Cell Integrity Requires the Adhesin Fig2p Eukaryot. Cell, October 1, 2002; 1(5): 811 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Drees, B. Sundin, E. Brazeau, J. P. Caviston, G.-C. Chen, W. Guo, K. G. Kozminski, M. W. Lau, J. J. Moskow, A. Tong, et al. A protein interaction map for cell polarity development J. Cell Biol., August 6, 2001; 154(3): 549 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Smits, H. van den Ende, and F. M. Klis Differential regulation of cell wall biogenesis during growth and development in yeast Microbiology, April 1, 2001; 147(4): 781 - 794. [Full Text]
|
|
|
|
 |
|
 A. F. Roth, B. Nelson, C. Boone, and N. G. Davis Asg7p-Ste3p Inhibition of Pheromone Signaling: Regulation of the Zygotic Transition to Vegetative Growth Mol. Cell. Biol., December 1, 2000; 20(23): 8815 - 8825. [Abstract] [Full Text]
|
|
|
|
|
|
M. Heiman and Walter Prm1p, a Pheromone-regulated Multispanning Membrane Protein, Facilitates Plasma Membrane Fusion during Yeast Mating J. Cell Biol., October 30, 2000; 151(3): 719 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Miller, D. Matheos, and M. D. Rose The Cortical Localization of the Microtubule Orientation Protein, Kar9p, Is Dependent upon Actin and Proteins Required for Polarization J. Cell Biol., March 8, 1999; 144(5): 963 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Maddox, E. Chin, A. Mallavarapu, E. Yeh, E.D. Salmon, and K. Bloom Microtubule Dynamics from Mating through the First Zygotic Division in the Budding Yeast Saccharomyces cerevisiae J. Cell Biol., March 8, 1999; 144(5): 977 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Johnson Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 54 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Sullivan, S. Biggins, and M. D. Rose The Yeast Centrin, Cdc31p, and the Interacting Protein Kinase, Kic1p, Are Required for Cell Integrity J. Cell Biol., November 2, 1998; 143(3): 751 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Elia and L. Marsh A Role for a Protease in Morphogenic Responses during Yeast Cell Fusion J. Cell Biol., September 21, 1998; 142(6): 1473 - 1485. [Abstract] [Full Text] [PDF]
|
|
