Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, S. H.
Right arrow Articles by Hong, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, S. H.
Right arrow Articles by Hong, W.

Vol. 9, Issue 6, 1549-1563, June 1998

Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome

Siew Heng Wong,* Tao Zhang,* Yue Xu,* V. Nathan Subramaniam,* Gareth Griffiths,dagger and Wanjin Hong*Dagger

 *Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore; and  dagger European Molecular Biology Laboratory, 69 Heidelberg, Federal Republic of Germany

Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. We report here the molecular, biochemical, and cell biological characterization of a novel member of the synaptobrevin/VAMP family. The amino acid sequence of endobrevin has 32, 33, and 31% identity to those of synaptobrevin/VAMP-1, synaptobrevin/VAMP-2, and cellubrevin, respectively. Membrane fractionation studies demonstrate that endobrevin is enriched in membrane fractions that are also enriched in the asialoglycoprotein receptor. Indirect immunofluorescence microscopy establishes that endobrevin is primarily associated with the perinuclear vesicular structures of the early endocytic compartment. The preferential association of endobrevin with the early endosome was further established by electron microscopy (EM) immunogold labeling. In vitro binding assays show that endobrevin interacts with immobilized recombinant alpha -SNAP fused to glutathione S-transferase (GST). Our results highlight the general importance of members of the synaptobrevin/VAMP protein family in membrane traffic and provide new avenues for future functional and mechanistic studies of this protein as well as the endocytotic pathway.

Molecular Biology of the Cell
Vol. 9, 1549-1563, June 1998
Copyright © 1998 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
T. H. Kloepper, C. N. Kienle, and D. Fasshauer
SNAREing the Basis of Multicellularity: Consequences of Protein Family Expansion during Evolution
Mol. Biol. Evol., September 1, 2008; 25(9): 2055 - 2068.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Tiwari, C.-C. Wang, C. Brochetta, G. Ke, F. Vita, Z. Qi, J. Rivera, M. R. Soranzo, G. Zabucchi, W. Hong, et al.
VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways
Blood, April 1, 2008; 111(7): 3665 - 3674.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. H. S. Ho, D. T. Cai, C.-C. Wang, D. Huang, and S. H. Wong
Vesicle-Associated Membrane Protein-8/Endobrevin Negatively Regulates Phagocytosis of Bacteria in Dendritic Cells
J. Immunol., March 1, 2008; 180(5): 3148 - 3157.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Pocard, A. Le Bivic, T. Galli, and C. Zurzolo
Distinct v-SNAREs regulate direct and indirect apical delivery in polarized epithelial cells
J. Cell Sci., September 15, 2007; 120(18): 3309 - 3320.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. Mittal, D. Dubey, K. Yamakawa, and S. Ganesh
Lafora disease proteins malin and laforin are recruited to aggresomes in response to proteasomal impairment
Hum. Mol. Genet., April 1, 2007; 16(7): 753 - 762.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. H. T. Tran, Q. Zeng, and W. Hong
VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes
J. Cell Sci., March 15, 2007; 120(6): 1028 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C.-C. Wang, H. Shi, K. Guo, C. P. Ng, J. Li, B. Qi Gan, H. Chien Liew, J. Leinonen, H. Rajaniemi, Z. Hong Zhou, et al.
VAMP8/Endobrevin as a General Vesicular SNARE for Regulated Exocytosis of the Exocrine System
Mol. Biol. Cell, March 1, 2007; 18(3): 1056 - 1063.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Q. Ren, H. K. Barber, G. L. Crawford, Z. A. Karim, C. Zhao, W. Choi, C.-C. Wang, W. Hong, and S. W. Whiteheart
Endobrevin/VAMP-8 Is the Primary v-SNARE for the Platelet Release Reaction
Mol. Biol. Cell, January 1, 2007; 18(1): 24 - 33.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Monzo, N. C. Gauthier, F. Keslair, A. Loubat, C. M. Field, Y. Le Marchand-Brustel, and M. Cormont
Clues to CD2-associated Protein Involvement in Cytokinesis
Mol. Biol. Cell, June 1, 2005; 16(6): 2891 - 2902.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. H. Wong, L. Santambrogio, and J. L. Strominger
Caspases and nitric oxide broadly regulate dendritic cell maturation and surface expression of class II MHC proteins
PNAS, December 21, 2004; 101(51): 17783 - 17788.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. L. TUMA and A. L. HUBBARD
Transcytosis: Crossing Cellular Barriers
Physiol Rev, July 1, 2003; 83(3): 871 - 932.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Martinez-Arca, V. Proux-Gillardeaux, P. Alberts, D. Louvard, and T. Galli
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles
J. Cell Sci., July 1, 2003; 116(13): 2805 - 2816.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zeng, T. T. H. Tran, H.-X. Tan, and W. Hong
The Cytoplasmic Domain of Vamp4 and Vamp5 Is Responsible for Their Correct Subcellular Targeting: THE N-TERMINAL EXTENSION OF VAMP4 CONTAINS A DOMINANT AUTONOMOUS TARGETING SIGNAL FOR THE TRANS-GOLGI NETWORK
J. Biol. Chem., June 13, 2003; 278(25): 23046 - 23054.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. Fratti, J. Chua, and V. Deretic
Cellubrevin Alterations and Mycobacterium tuberculosis Phagosome Maturation Arrest
J. Biol. Chem., May 3, 2002; 277(19): 17320 - 17326.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes
Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform
J. Cell Biol., February 18, 2002; 156(4): 653 - 664.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. Yeaman, K. K. Grindstaff, J. R. Wright, and W. J. Nelson
Sec6/8 complexes on trans-Golgi network and plasma membrane regulate late stages of exocytosis in mammalian cells
J. Cell Biol., November 12, 2001; 155(4): 593 - 604.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S Nagamatsu, Y Nakamichi, T Watanabe, S Matsushima, S Yamaguchi, J Ni, E Itagaki, and H Ishida
Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules
J. Cell Sci., January 1, 2001; 114(1): 219 - 227.
[Abstract] [PDF]

Home page
 Mol. Biol. CellHome page
W. Antonin, C. Holroyd, R. Tikkanen, S. Höning, and R. Jahn
The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes
Mol. Biol. Cell, October 1, 2000; 11(10): 3289 - 3298.
[Abstract] [Full Text]

Home page
 Mol. Biol. CellHome page
B. M. Mullock, C. W. Smith, G. Ihrke, N. A. Bright, M. Lindsay, E. J. Parkinson, D. A. Brooks, R. G. Parton, D. E. James, J. P. Luzio, et al.
Syntaxin 7 Is Localized to Late Endosome Compartments, Associates with Vamp 8, and Is Required for Late Endosome-Lysosome Fusion
Mol. Biol. Cell, September 1, 2000; 11(9): 3137 - 3153.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
W. Antonin, D. Riedel, and G. F. von Mollard
The SNARE Vti1a-beta Is Localized to Small Synaptic Vesicles and Participates in a Novel SNARE Complex
J. Neurosci., August 1, 2000; 20(15): 5724 - 5732.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Paumet, J. Le Mao, S. Martin, T. Galli, B. David, U. Blank, and M. Roa
Soluble NSF Attachment Protein Receptors (SNAREs) in RBL-2H3 Mast Cells: Functional Role of Syntaxin 4 in Exocytosis and Identification of a Vesicle-Associated Membrane Protein 8-Containing Secretory Compartment
J. Immunol., June 1, 2000; 164(11): 5850 - 5857.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Schiavo, M. Matteoli, and C. Montecucco
Neurotoxins Affecting Neuroexocytosis
Physiol Rev, April 1, 2000; 80(2): 717 - 766.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Prekeris, B. Yang, V. Oorschot, J. Klumperman, and R. H. Scheller
Differential Roles of Syntaxin 7 and Syntaxin 8 in Endosomal Trafficking
Mol. Biol. Cell, November 1, 1999; 10(11): 3891 - 3908.
[Abstract] [Full Text]

Home page
 Mol. Biol. CellHome page
C. Holroyd, U. Kistner, W. Annaert, and R. Jahn
Fusion of Endosomes Involved in Synaptic Vesicle Recycling
Mol. Biol. Cell, September 1, 1999; 10(9): 3035 - 3044.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. J. Hansen, W. Antonin, and J. M. Edwardson
Identification of SNAREs Involved in Regulated Exocytosis in the Pancreatic Acinar Cell
J. Biol. Chem., August 6, 1999; 274(32): 22871 - 22876.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Steegmaier, J. Klumperman, D. L. Foletti, J.-S. Yoo, and R. H. Scheller
Vesicle-associated Membrane Protein 4 is Implicated in Trans-Golgi Network Vesicle Trafficking
Mol. Biol. Cell, June 1, 1999; 10(6): 1957 - 1972.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. Fasshauer, W. Antonin, M. Margittai, S. Pabst, and R. Jahn
Mixed and Non-cognate SNARE Complexes. CHARACTERIZATION OF ASSEMBLY AND BIOPHYSICAL PROPERTIES
J. Biol. Chem., May 28, 1999; 274(22): 15440 - 15446.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. H. Wong, Y. Xu, T. Zhang, G. Griffiths, S. L. Lowe, V. N. Subramaniam, K. T. Seow, and W. Hong
GS32, a Novel Golgi SNARE of 32 kDa, Interacts Preferentially with Syntaxin 6
Mol. Biol. Cell, January 1, 1999; 10(1): 119 - 134.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
Q. Zeng, X. Si, H. Horstmann, Y. Xu, W. Hong, and C. J. Pallen
Prenylation-dependent Association of Protein-tyrosine Phosphatases PRL-1, -2, and -3 with the Plasma Membrane and the Early Endosome
J. Biol. Chem., July 7, 2000; 275(28): 21444 - 21452.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Wade, N. J. Bryant, L. M. Connolly, R. J. Simpson, J. P. Luzio, R. C. Piper, and D. E. James
Syntaxin 7 Complexes with Mouse Vps10p Tail Interactor 1b, Syntaxin 6, Vesicle-associated Membrane Protein (VAMP)8, and VAMP7 in B16 Melanoma Cells
J. Biol. Chem., June 1, 2001; 276(23): 19820 - 19827.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. L. Simpson, A. B. Maksymowych, and S. Hao
The Role of Zinc Binding in the Biological Activity of Botulinum Toxin
J. Biol. Chem., July 13, 2001; 276(29): 27034 - 27041.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes
Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform
J. Cell Biol., February 18, 2002; 156(4): 653 - 664.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]