![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 7, 1709-1723, July 1998
Department of Biology, Indiana University, Bloomington, Indiana
47405
Delta functions as a cell nonautonomous membrane-bound ligand that
binds to Notch, a cell-autonomous receptor, during cell fate
specification. Interaction between Delta and Notch leads to signal
transduction and elicitation of cellular responses. During our
investigations to further understand the biochemical mechanism by which
Delta signaling is regulated, we have identified four Delta isoforms in
Drosophila embryonic and larval extracts. We have
demonstrated that at least one of the smaller isoforms, Delta S,
results from proteolysis. Using antibodies to the Delta extracellular
and intracellular domains in colocalization experiments, we have found
that at least three Delta isoforms exist in vivo, providing the first
evidence that multiple forms of Delta exist during development.
Finally, we demonstrate that Delta is a transmembrane ligand that can
be taken up by Notch-expressing Drosophila cultured cells. Cell culture experiments imply that full-length Delta is taken
up by Notch-expressing cells. We present evidence that suggests this
uptake occurs by a nonphagocytic mechanism.
This article has been cited by other articles:
|
|
 |
|
  V. Ivan, G. de Voer, D. Xanthakis, K. M. Spoorendonk, V. Kondylis, and C. Rabouille Drosophila Sec16 Mediates the Biogenesis of tER Sites Upstream of Sar1 through an Arginine-Rich Motif Mol. Biol. Cell, October 1, 2008; 19(10): 4352 - 4365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Cherbas and P. Cherbas Drosophila Cell Culture and Transformation CSH Protocols, July 1, 2007; 2007(16): pdb.top6 - pdb.top6. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. T. Nichols, A. Miyamoto, S. L. Olsen, B. D'Souza, C. Yao, and G. Weinmaster DSL ligand endocytosis physically dissociates Notch1 heterodimers before activating proteolysis can occur J. Cell Biol., February 12, 2007; 176(4): 445 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Commisso and G. L. Boulianne The NHR1 Domain of Neuralized Binds Delta and Mediates Delta Trafficking and Notch Signaling Mol. Biol. Cell, January 1, 2007; 18(1): 1 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kondylis, K. M. Spoorendonk, and C. Rabouille dGRASP Localization and Function in the Early Exocytic Pathway in Drosophila S2 Cells Mol. Biol. Cell, September 1, 2005; 16(9): 4061 - 4072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Le Borgne, A. Bardin, and F. Schweisguth The roles of receptor and ligand endocytosis in regulating Notch signaling Development, April 15, 2005; 132(8): 1751 - 1762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Trifonova, D. Small, D. Kacer, D. Kovalenko, V. Kolev, A. Mandinova, R. Soldi, L. Liaw, I. Prudovsky, and T. Maciag The non-transmembrane form of Delta1 but not of jagged1 Induces normal migratory behavior accompanied by FGF receptor 1-dependent transformation J. Biol. Chem., February 9, 2004; (2004) 300564200. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Genissel, T. Pastinen, A. Dowell, T. F. C. Mackay, and A. D. Long No Evidence for an Association Between Common Nonsynonymous Polymorphisms in Delta and Bristle Number Variation in Natural and Laboratory Populations of Drosophila melanogaster Genetics, January 1, 2004; 166(1): 291 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Rawlins, B. Lovegrove, and A. P. Jarman Echinoid facilitates Notch pathway signalling during Drosophila neurogenesis through functional interaction with Delta Development, December 29, 2003; 130(26): 6475 - 6484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kondylis and C. Rabouille A novel role for dp115 in the organization of tER sites in Drosophila J. Cell Biol., July 21, 2003; 162(2): 185 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Six, D. Ndiaye, Y. Laabi, C. Brou, N. Gupta-Rossi, A. Israel, and F. Logeat The Notch ligand Delta1 is sequentially cleaved by an ADAM protease and {gamma}-secretase PNAS, June 24, 2003; 100(13): 7638 - 7643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Grandbarbe, J. Bouissac, M. Rand, M. Hrabe de Angelis, S. Artavanis-Tsakonas, and E. Mohier Delta-Notch signaling controls the generation of neurons/glia from neural stem cells in a stepwise process Development, April 1, 2003; 130(7): 1391 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mishra-Gorur, M. D. Rand, B. Perez-Villamil, and S. Artavanis-Tsakonas Down-regulation of Delta by proteolytic processing J. Cell Biol., October 28, 2002; 159(2): 313 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ghiglione, E. A. Bach, Y. Paraiso, K. L. Carraway III, S. Noselli, and N. Perrimon Mechanism of activation of the Drosophila EGF Receptor by the TGF{alpha} ligand Gurken during oogenesis Development, January 1, 2002; 129(1): 175 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Incardona, J. H. Lee, C. P. Robertson, K. Enga, R. P. Kapur, and H. Roelink Receptor-mediated endocytosis of soluble and membrane-tethered Sonic hedgehog by Patched-1 PNAS, October 4, 2000; (2000) 220251997. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. D. Rand, L. M. Grimm, S. Artavanis-Tsakonas, V. Patriub, S. C. Blacklow, J. Sklar, and J. C. Aster Calcium Depletion Dissociates and Activates Heterodimeric Notch Receptors Mol. Cell. Biol., March 1, 2000; 20(5): 1825 - 1835. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. Han, Q. Ye, and M. A. S. Moore A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells Blood, March 1, 2000; 95(5): 1616 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Parks, K. Klueg, J. Stout, and M. Muskavitch Ligand endocytosis drives receptor dissociation and activation in the Notch pathway Development, January 4, 2000; 127(7): 1373 - 1385. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Artavanis-Tsakonas, M. D. Rand, and R. J. Lake Notch Signaling: Cell Fate Control and Signal Integration in Development Science, April 30, 1999; 284(5415): 770 - 776. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sunio, A. B. Metcalf, and H. Krämer Genetic Dissection of Endocytic Trafficking in Drosophila Using a Horseradish Peroxidase-Bride of Sevenless Chimera: hook Is Required for Normal Maturation of Multivesicular Endosomes Mol. Biol. Cell, April 1, 1999; 10(4): 847 - 859. [Abstract] [Full Text]
|
|
|
|
|
|
H. Krämer and M. Phistry Genetic Analysis of hook, a Gene Required for Endocytic Trafficking in Drosophila Genetics, February 1, 1999; 151(2): 675 - 684. [Abstract] [Full Text]
|
|
|
|
 |
|
 J Schlondorff and C. Blobel Metalloprotease-disintegrins: modular proteins capable of promoting cell-cell interactions and triggering signals by protein-ectodomain shedding J. Cell Sci., January 11, 1999; 112(21): 3603 - 3617. [Abstract] [PDF]
|
|
|
|
|
|
K. Klueg and M. Muskavitch Ligand-receptor interactions and trans-endocytosis of Delta, Serrate and Notch: members of the Notch signalling pathway in Drosophila J. Cell Sci., January 10, 1999; 112(19): 3289 - 3297. [Abstract] [PDF]
|
|
|
|
|
|
D. Sherwood and D. McClay LvNotch signaling mediates secondary mesenchyme specification in the sea urchin embryo Development, January 4, 1999; 126(8): 1703 - 1713. [Abstract] [PDF]
|
|
|
|
|
|
H. Qi, M. D. Rand, X. Wu, N. Sestan, W. Wang, P. Rakic, T. Xu, and S. Artavanis-Tsakonas Processing of the Notch Ligand Delta by the Metalloprotease Kuzbanian Science, January 1, 1999; 283(5398): 91 - 94. [Abstract] [Full Text]
|
|
|
|
|
|
J. P. Incardona, J. H. Lee, C. P. Robertson, K. Enga, R. P. Kapur, and H. Roelink Receptor-mediated endocytosis of soluble and membrane-tethered Sonic hedgehog by Patched-1 PNAS, October 24, 2000; 97(22): 12044 - 12049. [Abstract] [Full Text] [PDF]
|
|
