![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 9, Issue 7, 1847-1861, July 1998
Section of Molecular and Cellular Biology, University of
California, Davis, Davis, California 95616
Microtubules are dynamic structures whose proper rearrangement
during the cell cycle is essential for the positioning of membranes during interphase and for chromosome segregation during mitosis. The previous discovery of a cyclin B/cdc2-activated
microtubule-severing activity in M-phase Xenopus egg
extracts suggested that a microtubule-severing protein might play an
important role in cell cycle-dependent changes in microtubule dynamics
and organization. However, the isolation of three different
microtubule-severing proteins, p56, EF1
, and katanin, has only
confused the issue because none of these proteins is directly activated
by cyclin B/cdc2. Here we use immunodepletion with antibodies specific
for a vertebrate katanin homologue to demonstrate that katanin is
responsible for the majority of M-phase severing activity in
Xenopus eggs. This result suggests that katanin is
responsible for changes in microtubules occurring at mitosis. Immunofluorescence analysis demonstrated that katanin is concentrated at a microtubule-dependent structure at mitotic spindle poles in
Xenopus A6 cells and in human fibroblasts, suggesting a
specific role in microtubule disassembly at spindle poles.
Surprisingly, katanin was also found in adult mouse brain, indicating
that katanin may have other functions distinct from its mitotic role.
This article has been cited by other articles:
|
|
 |
|
  H. Sudo and Y. Maru LAPSER1/LZTS2: a pluripotent tumor suppressor linked to the inhibition of katanin-mediated microtubule severing Hum. Mol. Genet., August 15, 2008; 17(16): 2524 - 2540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sharma, J. Bryant, D. Wloga, R. Donaldson, R. C. Davis, M. Jerka-Dziadosz, and J. Gaertig Katanin regulates dynamics of microtubules and biogenesis of motile cilia J. Cell Biol., September 7, 2007; 178(6): 1065 - 1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhang, G. C. Rogers, D. W. Buster, and D. J. Sharp Three microtubule severing enzymes contribute to the "Pacman-flux" machinery that moves chromosomes J. Cell Biol., April 23, 2007; 177(2): 231 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. McNally, A. Audhya, K. Oegema, and F. J. McNally Katanin controls mitotic and meiotic spindle length J. Cell Biol., December 18, 2006; 175(6): 881 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. DeBella, A. Hayashi, and L. S. Rose LET-711, the Caenorhabditis elegans NOT1 Ortholog, Is Required for Spindle Positioning and Regulation of Microtubule Length in Embryos Mol. Biol. Cell, November 1, 2006; 17(11): 4911 - 4924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Komorisono, M. Ueguchi-Tanaka, I. Aichi, Y. Hasegawa, M. Ashikari, H. Kitano, M. Matsuoka, and T. Sazuka Analysis of the Rice Mutant dwarf and gladius leaf 1. Aberrant Katanin-Mediated Microtubule Organization Causes Up-Regulation of Gibberellin Biosynthetic Genes Independently of Gibberellin Signaling Plant Physiology, August 1, 2005; 138(4): 1982 - 1993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Evans, E. R. Gomes, S. M. Reisenweber, G. G. Gundersen, and B. P. Lauring Linking axonal degeneration to microtubule remodeling by Spastin-mediated microtubule severing J. Cell Biol., February 14, 2005; 168(4): 599 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Errico, P. Claudiani, M. D'Addio, and E. I. Rugarli Spastin interacts with the centrosomal protein NA14, and is enriched in the spindle pole, the midbody and the distal axon Hum. Mol. Genet., September 15, 2004; 13(18): 2121 - 2132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. T. O'Toole, K. L. McDonald, J. Mantler, J. R. McIntosh, A. A. Hyman, and T. Muller-Reichert Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans J. Cell Biol., November 10, 2003; 163(3): 451 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Marcus, W. Li, H. Ma, and R. J. Cyr A Kinesin Mutant with an Atypical Bipolar Spindle Undergoes Normal Mitosis Mol. Biol. Cell, April 1, 2003; 14(4): 1717 - 1726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Amayed, D. Pantaloni, and M.-F. Carlier The Effect of Stathmin Phosphorylation on Microtubule Assembly Depends on Tubulin Critical Concentration J. Biol. Chem., June 14, 2002; 277(25): 22718 - 22724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Mahjoub, B. Montpetit, L. Zhao, R. J. Finst, B. Goh, A. C. Kim, and L. M. Quarmby The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation J. Cell Sci., April 15, 2002; 115(8): 1759 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. O. Wasteneys Microtubule organization in the green kingdom: chaos or self-order? J. Cell Sci., January 4, 2002; 115(7): 1345 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Buster, K. McNally, and F. J. McNally Katanin inhibition prevents the redistribution of {gamma}-tubulin at mitosis J. Cell Sci., January 3, 2002; 115(5): 1083 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Errico, A. Ballabio, and E. I. Rugarli Spastin, the protein mutated in autosomal dominant hereditary spastic paraplegia, is involved in microtubule dynamics Hum. Mol. Genet., January 1, 2002; 11(2): 153 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Burk, B. Liu, R. Zhong, W. H. Morrison, and Z.-H. Ye A Katanin-like Protein Regulates Normal Cell Wall Biosynthesis and Cell Elongation PLANT CELL, April 1, 2001; 13(4): 807 - 828. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Srayko, D. W. Buster, O. A. Bazirgan, F. J. McNally, and P. E. Mains MEI-1/MEI-2 katanin-like microtubule severing activity is required for Caenorhabditis elegans meiosis Genes & Dev., May 1, 2000; 14(9): 1072 - 1084. [Abstract] [Full Text]
|
|
|
|
|
|
L Quarmby Cellular Samurai: katanin and the severing of microtubules J. Cell Sci., January 8, 2000; 113(16): 2821 - 2827. [Abstract] [PDF]
|
|
|
|
|
|
K. McNally, O. Bazirgan, and F. McNally Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin J. Cell Sci., January 5, 2000; 113(9): 1623 - 1633. [Abstract] [PDF]
|
|
|
|
 |
|
 E. W. Dent, J. L. Callaway, G. Szebenyi, P. W. Baas, and K. Kalil Reorganization and Movement of Microtubules in Axonal Growth Cones and Developing Interstitial Branches J. Neurosci., October 15, 1999; 19(20): 8894 - 8908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Shuster and D. R. Burgess Parameters That Specify the Timing of Cytokinesis J. Cell Biol., September 6, 1999; 146(5): 981 - 992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Ahmad, W. Yu, F. J. McNally, and P. W. Baas An Essential Role for Katanin in Severing Microtubules in the Neuron J. Cell Biol., April 19, 1999; 145(2): 305 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kuntziger, O. Gavet, A. Sobel, and M. Bornens Differential Effect of Two Stathmin/Op18 Phosphorylation Mutants on Xenopus Embryo Development J. Biol. Chem., June 15, 2001; 276(25): 22979 - 22984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Eichenmuller, P. Everley, J. Palange, D. Lepley, and K. A. Suprenant The Human EMAP-like Protein-70 (ELP70) Is a Microtubule Destabilizer That Localizes to the Mitotic Apparatus J. Biol. Chem., January 4, 2002; 277(2): 1301 - 1309. [Abstract] [Full Text] [PDF]
|
|
