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Vol. 9, Issue 8, 2069-2079, August 1998
2
Integrin Motif
and§
Departments of
*Microbiology,
Killing of human cells by the parasite Entamoeba
histolytica requires adherence via an amebic cell surface
lectin. Lectin activity in the parasite is regulated by inside-out
signaling. The lectin cytoplasmic domain has sequence identity with a
region of the Medicine, and
§Pathology, University of Virginia, Charlottesville,
Virginia 22908
2 integrin cytoplasmic tail implicated in
regulation of integrin-mediated adhesion. Intracellular
expression of a fusion protein containing the cytoplasmic domain of the
lectin has a dominant negative effect on extracellular lectin-mediated
cell adherence. Mutation of the integrin-like sequence
abrogates the dominant negative effect. Amebae expressing the dominant
negative mutant are less virulent in an animal model of amebiasis.
These results suggest that inside-out signaling via the lectin
cytoplasmic domain may control the extracellular adhesive activity of
the amebic lectin and provide in vivo demonstration of the lectin's role in virulence.
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