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A more recent version of this article appeared on January 1, 2003
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Submitted on June 4, 2002
Revised on September 27, 2002
Accepted on October 3, 2002
1 Molecular and Cell Biology, Sunnybrook and Women's College Health Science Centre, 2075 Bayview Ave, Toronto, Ontario, Canada, M4N 3M5
2 Max Planck Institute for Biochemistry, Martinsried, 82152, Germany
* Corresponding author. E-mail address: jslingerland{at}med.miami.edu.
We show that p27 localization is cell cycle regulated and suggest that active CRM1/RanGTP-mediated nuclear export of p27 may be linked to cytoplasmic p27 proteolysis in early G1. p27 is nuclear in G0 and early G1 and appears transiently in the cytoplasm at the G1/S transition. Association of p27 with the exportin CRM1 was minimal in G0 and increased markedly during G1-to-S phase progression. Proteasome inhibition in mid G1 did not impair nuclear import of p27, but led to accumulation of p27 in the cytoplasm, suggesting that export precedes degradation for at least part of the cellular p27 pool. p27-CRM1 binding and nuclear export were inhibited by S10A mutation but not by T187A mutation. A putative NES in p27 is identified whose mutation reduced p27-CRM1 interaction, nuclear export and p27 degradation. LMB did not inhibit p27-CRM1 binding, nor did it prevent p27 export in vitro or in heterokaryon assays. Pre-binding of CRM1 to the HIV-1 Rev NES did not inhibit p27-CRM1 interaction, suggesting that p27 binds CRM1 at a non-LMB sensitive motif. LMB increased total cellular p27 and may do so indirectly though effects on other p27 regulatory proteins. These data suggest a model in which p27 undergoes active, CRM1-dependent nuclear export and cytoplasmic degradation in early G1. This would permit the incremental activation of cyclin E-Cdk2 leading to cyclin E-Cdk2 mediated T187 phosphorylation and p27 proteolysis in late G1 and S phase.
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