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A more recent version of this article appeared on January 1, 2003
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Submitted on July 15, 2002
Revised on September 30, 2002
Accepted on October 8, 2002
1 Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas (CSIC) / Departamento de Microbiologia y Genetica, Universidad de Salamanca, Edificio Departamental, 37007 Salamanca, Spain
* Corresponding author. E-mail address: piper{at}usal.es.
Schizosaccharomyces pombe cdc42+ regulates cell morphology and polarization of the actin cytoskeleton. Scd1p/Ral1p is the only described guanine nucleotide exchange factor (GEF) for Cdc42p in S. pombe. We have identified a new GEF, named Gef1p, specifically regulating Cdc42p. Gef1p binds to inactive Cdc42p but not to other Rho GTPases in two hybrid assays. Overexpression of gef1+ increases specifically the GTP-bound Cdc42p, and Gef1p is capable of stimulating guanine nucleotide exchange of Cdc42p in vitro. Overexpression of gef1+ causes changes in cell morphology similar to those caused by overexpression of the constitutively active cdc42G12V allele.
Gef1p localises to the septum. gef1+ deletion is viable but causes a mild cell elongation and defects in bipolar growth and septum formation, suggesting a role for Gef1p in the control of cell polarity and cytokinesis. The double mutant gef1
scd1 is not viable, indicating that they share an essential function as Cdc42p activators. However, both deletion and overexpression of either gef1+ or scd1+ causes different morphological phenotypes, which suggest different functions. Genetic evidence revealed a link between Gef1p and the signalling pathway of Shk1/Orb2p and Orb6p. By contrast, no genetic interaction between Gef1p and Shk2p-Mkh1p pathway was observed.
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