![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on November 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on February 20, 2003
Revised on June 13, 2003
Accepted on July 3, 2003
1 Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge MA 02139
* Corresponding author. E-mail address: angelika{at}mit.edu.
In budding yeast, the protein phosphatase Cdc14 controls exit from mitosis. Its activity is regulated by a competitive inhibitor Cfi1/Net1, which binds to and sequesters Cdc14 in the nucleolus. During anaphase Cdc14 is released from its inhibitor by the action of two regulatory networks. The Cdc Fourteen Early Anaphase Release (FEAR) network initiates Cdc14 release from Cfi1/Net1 during early anaphase, and the Mitotic Exit Network (MEN) promotes Cdc14 release during late anaphase. Here we investigate the relationship among FEAR network components and propose an order in which they function to promote Cdc14 release from the nucleolus. Furthermore, we examine the role of the protein kinase Cdc5, which is a component of both the FEAR network and the MEN, in Cdc14 release from the nucleolus. We find that overexpression of CDC5 led to Cdc14 release from the nucleolus in S phase-arrested cells, which correlated with the appearance of phosphorylated forms of Cdc14 and Cfi1/Net1. Cdc5 promotes Cdc14 phosphorylation and, by stimulating the MEN, Cfi1/Net1 phosphorylation. Furthermore, we suggest that Cdc14 release from the nucleolus only occurs when Cdc14 and Cfi1/Net1 are both phosphorylated.
This article has been cited by other articles:
|
|
 |
|
  M. Jwa, J.-h. Kim, and C. S.M. Chan Regulation of Sli15/INCENP, kinetochore, and Cdc14 phosphatase functions by the ribosome biogenesis protein Utp7 J. Cell Biol., September 22, 2008; 182(6): 1099 - 1111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Movshovich, V. Fridman, A. Gerson-Gurwitz, I. Shumacher, I. Gertsberg, A. Fich, M. A. Hoyt, B. Katz, and L. Gheber Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells J. Cell Sci., August 1, 2008; 121(15): 2529 - 2539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Visintin, B. N. Tomson, R. Rahal, J. Paulson, M. Cohen, J. Taunton, A. Amon, and R. Visintin APC/C-Cdh1-mediated degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus Genes & Dev., January 1, 2008; 22(1): 79 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yuan, H. Hu, Z. Guo, G. Fu, A. P. Shaw, R. Hu, and X. Yao Phospho-regulation of HsCdc14A By Polo-like Kinase 1 Is Essential for Mitotic Progression J. Biol. Chem., September 14, 2007; 282(37): 27414 - 27423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Yabuta, N. Okada, A. Ito, T. Hosomi, S. Nishihara, Y. Sasayama, A. Fujimori, D. Okuzaki, H. Zhao, M. Ikawa, et al. Lats2 Is an Essential Mitotic Regulator Required for the Coordination of Cell Division J. Biol. Chem., June 29, 2007; 282(26): 19259 - 19271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eluere, N. Offner, I. Varlet, O. Motteux, L. Signon, A. Picard, E. Bailly, and M.-N. Simon Compartmentalization of the functions and regulation of the mitotic cyclin Clb2 in S. cerevisiae J. Cell Sci., February 15, 2007; 120(4): 702 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-T. Chen, M.-P. Peli-Gulli, V. Simanis, and D. McCollum S. pombe FEAR protein orthologs are not required for release of Clp1/Flp1 phosphatase from the nucleolus during mitosis J. Cell Sci., November 1, 2006; 119(21): 4462 - 4466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Reiser, K. E. D'Aquino, L.-S. Ee, and A. Amon The Stress-activated Mitogen-activated Protein Kinase Signaling Cascade Promotes Exit from Mitosis Mol. Biol. Cell, July 1, 2006; 17(7): 3136 - 3146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Clemente-Blanco, A. Gonzalez-Novo, F. Machin, D. Caballero-Lima, L. Aragon, M. Sanchez, C. R. V. de Aldana, J. Jimenez, and J. Correa-Bordes The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans J. Cell Sci., March 15, 2006; 119(6): 1130 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stoepel, M. A. Ottey, C. Kurischko, P. Hieter, and F. C. Luca The Mitotic Exit Network Mob1p-Dbf2p Kinase Complex Localizes to the Nucleus and Regulates Passenger Protein Localization Mol. Biol. Cell, December 1, 2005; 16(12): 5465 - 5479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. M. van de Weerdt, M. A. T. M. van Vugt, C. Lindon, J. J. W. Kauw, M. J. Rozendaal, R. Klompmaker, R. M. F. Wolthuis, and R. H. Medema Uncoupling Anaphase-Promoting Complex/Cyclosome Activity from Spindle Assembly Checkpoint Control by Deregulating Polo-Like Kinase 1 Mol. Cell. Biol., March 1, 2005; 25(5): 2031 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D'Amours and A. Amon At the interface between signaling and executing anaphase--Cdc14 and the FEAR network Genes & Dev., November 1, 2004; 18(21): 2581 - 2595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Chen, L. Calzone, A. Csikasz-Nagy, F. R. Cross, B. Novak, and J. J. Tyson Integrative Analysis of Cell Cycle Control in Budding Yeast Mol. Biol. Cell, August 1, 2004; 15(8): 3841 - 3862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Azzam, S. L. Chen, W. Shou, A. S. Mah, G. Alexandru, K. Nasmyth, R. S. Annan, S. A. Carr, and R. J. Deshaies Phosphorylation by Cyclin B-Cdk Underlies Release of Mitotic Exit Activator Cdc14 from the Nucleolus Science, July 23, 2004; 305(5683): 516 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hofken and E. Schiebel Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2 J. Cell Biol., January 19, 2004; 164(2): 219 - 231. [Abstract] [Full Text] [PDF]
|
|
