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MBC in Press, published online ahead of print June 13, 2003
Mol. Biol. Cell 10.1091/mbc.E03-02-0109

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Submitted on February 25, 2003
Revised on May 2, 2003
Accepted on May 22, 2003

Ectopic expression of atRSZ33 reveals its function in splicing and causes pleiotropic changes in development

Maria Kalyna1, Sergiy Lopato1, and Andrea Barta1*

1 Institute of Medical Biochemistry, Vienna BioCenter, University of Vienna, Dr. Bohrgasse 9/3, A-1030 Vienna, Austria

* Corresponding author. E-mail address: Andrea{at}bch.univie.ac.at.

Splicing provides an additional level in the regulation of gene expression and contributes to proteome diversity. Here we report the functional characterization of a recently described plant-specific protein, atRSZ33, which has characteristic features of a serine/arginine-rich (SR) protein and the ability to interact with other splicing factors implying that this protein might be involved in constitutive and/or alternative splicing. Overexpression of atRSZ33 leads to alteration of splicing patterns of atSRp30 and atSRp34/SR1 indicating that atRSZ33 is indeed a splicing factor. Moreover, atRSZ33 is a regulator of its own expression, as splicing of its premRNA is changed in transgenic plants. Investigations by promoter-{beta}-glucuronidase (GUS) fusion and in situ hybridization revealed that atRSZ33 is expressed during embryogenesis and early stages of seedling formation, as well as in flower and root development. Ectopic expression of atRSZ33 caused pleiotropic changes in plant development resulting in increased cell expansion and changed polarization of cell elongation and division. In addition, changes in activity of an auxin-responsive promoter suggest that auxin signaling is disturbed in these transgenic plants.




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