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A more recent version of this article appeared on November 1, 2003
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Submitted on March 20, 2003
Revised on June 25, 2003
Accepted on June 26, 2003
1 The Wellcome Centre for Molecular Parasitology, The University of Glasgow, Anderson College, 56 Dumbarton Rd., Glasgow, G11 6NU, UK
* Corresponding author. E-mail address: i.johnstone{at}vet.gla.ac.uk.
SEC-23 is a component of COPII coated vesicles involved in the ER
to Golgi transport pathway of eukaryotes. During postembryonic life,
Caenorhabditis elegans is surrounded by a collagenous
exoskeleton termed the cuticle. From a screen for mutants defective in
cuticle secretion, we identified and characterized a
sec-23 mutant of C. elegans. By sequence
homology, C. elegans has only the single
sec-23 gene described here. In addition to the cuticle
secretion defect, mutants fail to complete embryonic morphogenesis.
However, they progress through the earlier stages of embryogenesis,
including gastrulation, and achieve substantial morphogenesis 
before
death. We demonstrated a maternal component of SEC-23 function,
sufficient for progression through the earlier stages of embryogenesis
and explaining the limited phenotype of the zygotic mutant. By
RNA-mediated interference (RNAi), we investigated the effects of
perturbing COPII function during various postembryonic stages. During
larval stages, major defects in cuticle synthesis and molting were
observed. In the adult hermaphrodite, reduction of SEC-23 function by
RNAi caused a rapid onset of sterility, with defects in oogenesis
including early maturation of the germline nuclei, probably a result of
the observed loss of the GLP-1 receptor from the membrane surfaces
adjacent to the developing germline nuclei.
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