![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on January 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on March 31, 2003
Revised on August 27, 2003
Accepted on August 28, 2003
1 Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, MD 21210
* Corresponding author. E-mail address: zheng{at}ciwemb.edu.
The centrosome in animal cells provides a major
microtubule-nucleating site that regulates the microtubule cytoskeleton
temporally and spatially throughout the cell cycle. We report the
identification in Drosophila melanogaster of a large
coiled-coil centrosome protein that can bind to calmodulin (CaM).
Biochemical studies reveal that this novel Drosophila
centrosome protein, CP309 (Centrosome
Protein of 309 kDa), cofractionates with
the 
-tubulin ring complex (TuRC) and the centrosome complementing
activity. We show that CP309 is required for microtubule nucleation
mediated by centrosomes and that it interacts with the -tubulin
small complex (TuSC). These findings suggest that the microtubule
nucleating activity of the centrosome requires the function of CP309.
This article has been cited by other articles:
|
|
 |
|
  D. Ducat, S.-i. Kawaguchi, H. Liu, J. R. Yates III, and Y. Zheng Regulation of Microtubule Assembly and Organization in Mitosis by the AAA+ ATPase Pontin Mol. Biol. Cell, July 1, 2008; 19(7): 3097 - 3110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Turner, M. T. Levine, M. L. Eckert, and D. J. Begun Genomic Analysis of Adaptive Differentiation in Drosophila melanogaster Genetics, May 1, 2008; 179(1): 455 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wiese Distinct Dgrip84 Isoforms Correlate with Distinct {gamma}-Tubulins in Drosophila Mol. Biol. Cell, January 1, 2008; 19(1): 368 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Sillibourne, B. Delaval, S. Redick, M. Sinha, and S. J. Doxsey Chromatin Remodeling Proteins Interact with Pericentrin to Regulate Centrosome Integrity Mol. Biol. Cell, September 1, 2007; 18(9): 3667 - 3680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Minakhina, M. Druzhinina, and R. Steward Zfrp8, the Drosophila ortholog of PDCD2, functions in lymph gland development and controls cell proliferation Development, July 1, 2007; 134(13): 2387 - 2396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Mirouse, E. Formstecher, and J.-L. Couderc Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila Development, October 15, 2006; 133(20): 4005 - 4013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Vogt, I. Koch, H. Schwarz, F. Schnorrer, and C. Nusslein-Volhard The {gamma}TuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline Development, October 15, 2006; 133(20): 3963 - 3972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Haren, M.-H. Remy, I. Bazin, I. Callebaut, M. Wright, and A. Merdes NEDD1-dependent recruitment of the {gamma}-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly J. Cell Biol., February 13, 2006; 172(4): 505 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Verollet, N. Colombie, T. Daubon, H.-M. Bourbon, M. Wright, and B. Raynaud-Messina Drosophila melanogaster {gamma}-TuRC is dispensable for targeting {gamma}-tubulin to the centrosome and microtubule nucleation J. Cell Biol., February 13, 2006; 172(4): 517 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Januschke, L. Gervais, L. Gillet, G. Keryer, M. Bornens, and A. Guichet The centrosome-nucleus complex and microtubule organization in the Drosophila oocyte Development, January 1, 2006; 133(1): 129 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Colombie, C. Verollet, P. Sampaio, A. Moisand, C. Sunkel, H.-M. Bourbon, M. Wright, and B. Raynaud-Messina The Drosophila {gamma}-Tubulin Small Complex Subunit Dgrip84 Is Required for Structural and Functional Integrity of the Spindle Apparatus Mol. Biol. Cell, January 1, 2006; 17(1): 272 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Samejima, P. C. C. Lourenco, H. A. Snaith, and K. E. Sawin Fission Yeast mto2p Regulates Microtubule Nucleation by the Centrosomin-related Protein mto1p Mol. Biol. Cell, June 1, 2005; 16(6): 3040 - 3051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rapley, J. E. Baxter, J. Blot, S. L. Wattam, M. Casenghi, P. Meraldi, E. A. Nigg, and A. M. Fry Coordinate Regulation of the Mother Centriole Component Nlp by Nek2 and Plk1 Protein Kinases Mol. Cell. Biol., February 15, 2005; 25(4): 1309 - 1324. [Abstract] [Full Text] [PDF]
|
|
