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A more recent version of this article appeared on December 1, 2003
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Submitted on April 15, 2003
Revised on July 9, 2003
Accepted on July 28, 2003
1 Department of Cell Biology, Howard Hughes Medical Institute, Yale University School of Medicine, 333 Cedar Street, New Haven CT 06510
* Corresponding author. E-mail address: peter.novick{at}yale.edu.
Sec3p is a component of the exocyst complex that tethers secretory
vesicles to the plasma membrane at exocytic sites in preparation for
fusion. Unlike all other exocyst structural genes, SEC3
is not essential for growth. Cells lacking Sec3p grow and secrete
surprisingly well at 25°C, however late markers of secretion, such as
the vesicle marker Sec4p and the exocyst subunit Sec8p, localize more
diffusely within the bud. Furthermore, sec3
cells are
strikingly round relative to wild-type cells and are unable to form
pointed mating projections in response to 
factor. These phenotypes
support the proposed role of Sec3p as a spatial landmark for secretion.
We also find that cells lacking Sec3p exhibit a dramatic defect in the
inheritance of cortical ER into the bud, while the inheritance of
mitochondria and Golgi is unaffected. Overexpression of Sec3p results
in a prominent patch of the ER marker Sec61p-GFP at the bud tip.
Cortical ER inheritance in yeast has been suggested to involve the
capture of ER tubules at the bud tip. Sec3p may act in this process as
a spatial landmark for cortical ER inheritance.
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