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A more recent version of this article appeared on December 1, 2003
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Submitted on April 29, 2003
Revised on July 24, 2003
Accepted on August 13, 2003
1 Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242-1324
2 Department of Medical Microbiology and Immunology, College of Medicine and Public Health, Ohio State University, Columbus, Ohio 43210
* Corresponding author. E-mail address: david-soll{at}uiowa.edu.
The cell wall protein Hwp1 was originally demonstrated to be
expressed exclusively in hyphae of Candida albicans and
cross-linked to human epithelium by mammalian transglutaminase. Hwp1 is
expressed on the walls of hyphae formed by a/
,
a/a and / cells. Hence, it is expressed on
hyphae independently of mating type. However, Hwp1 is selectively
expressed on the wall of conjugation tubes formed by
a/a cells, but not / cells, in the mating
process. This was demonstrated in all possible cross-es between four
unrelated natural a/a strains and four unrelated
/ strains. In zygotes, Hwp1 is restricted to that portion of the
wall of the conjugation bridge contributed by the
a/a parent cell. Hwp1 staining further revealed
that the first daughter bud that emerges from the conjugation bridge
does so from the a/a-contributed portion. Hwp1
expression and localization during the mating process is, therefore,
mating type-specific, opaque phase-specific and -pheromone-induced.
These results indicate that the mating type-specific contributions to
the conjugation bridge during the mating process in Candida
albicans are qualitatively and functionally distinct, and that the
a/a portion of the bridge, which selectively
contains Hwp1, bears the first daughter cell in the mating process.
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