Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print December 10, 2003
Mol. Biol. Cell 10.1091/mbc.E03-05-0301

A more recent version of this article appeared on March 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-05-0301v1
15/3/1124    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boyer, L.
Right arrow Articles by Fabbri, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boyer, L.
Right arrow Articles by Fabbri, A.

Submitted on May 15, 2003
Revised on August 13, 2003
Accepted on October 24, 2003

Rac GTPase instructs NF-kB activation by conveying the SCF complex and IkB{alpha} to the ruffling membranes

Laurent Boyer1, Sara Travaglione2, Loredana Falzano2, Nils C. Gauthier1, Michel R. Popoff3, Emmanuel Lemichez1, Carla Fiorentini4, and Alessia Fabbri4*

1 INSERM U452, IFR50, Faculté de Médecine, 28 Avenue de Valombrose, 06107 Nice, France
2 Laboratory of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy; New affiliation: Department of Drug Research and Evaluation
3 CNR Anaerobies, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France
4 Laboratory of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy; New affiliation: Department of Drug Research and Evaluation; C. Fiorentini and A. Fabbri were principal investigators

* Corresponding author. E-mail address: alessia.fabbri{at}iss.it.

NF-{kappa}B is an ubiquitously expressed transcription factor that plays a central role in directing a vast range of cellular functions. Its activation is controlled by the Rac GTPase and relies on the coordinated cooperation of the E3-ligase complex SCF{beta}{beta}TrCP, composed by Skp-1/Cullin-1, Rbx/Roc1 and the {beta}-TrCP proteins. Recently, Cullin-1 has been reported to form a complex with the activated Rac GTPase. Here we show that the specific activation of the Rac GTPase, besides directing its own positioning, induces the relocalization of the SCF component Cullin-1 to the ruffling membranes. This occurred only if the ruffles were stimulated by the Rac GTPase and was accompanied by the repositioning to the same intracellular compartment of the SCF protein Skp-1 and the ubiquitin-like molecule Nedd-8. The SCF substrate IkB{alpha} was also directed to the ruffling membranes in a Rac-dependent way. The novelty of these findings is in respect to the demonstration that the correct positioning at the ruffling membranes is crucial for the subsequent series of events that leads to IkB{alpha} proteasomal degradation and the resultant activation of NF-{kappa}B. Consequently, this points to the role of Rac as a docking molecule in NF-{kappa}B activation.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Ozawa and H. Kobori
Crucial role of Rho-nuclear factor-{kappa}B axis in angiotensin II-induced renal injury
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F100 - F109.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. G. Miraglia, S. Travaglione, S. Meschini, L. Falzano, P. Matarrese, M. G. Quaranta, M. Viora, C. Fiorentini, and A. Fabbri
Cytotoxic Necrotizing Factor 1 Prevents Apoptosis via the Akt/I{kappa}B Kinase Pathway: Role of Nuclear Factor-{kappa}B and Bcl-2
Mol. Biol. Cell, July 1, 2007; 18(7): 2735 - 2744.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Diana, G. Valentini, S. Travaglione, L. Falzano, M. Pieri, C. Zona, S. Meschini, A. Fabbri, and C. Fiorentini
Enhancement of learning and memory after activation of cerebral Rho GTPases
PNAS, January 9, 2007; 104(2): 636 - 641.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. Falzano, P. Filippini, S. Travaglione, A. G. Miraglia, A. Fabbri, and C. Fiorentini
Escherichia coli Cytotoxic Necrotizing Factor 1 Blocks Cell Cycle G2/M Transition in Uroepithelial Cells
Infect. Immun., July 1, 2006; 74(7): 3765 - 3772.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Matos and P. Jordan
Rac1, but Not Rac1B, Stimulates RelB-mediated Gene Transcription in Colorectal Cancer Cells
J. Biol. Chem., May 12, 2006; 281(19): 13724 - 13732.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
W. Malorni and C. Fiorentini
Is the Rac GTPase-activating toxin CNF1 a smart hijacker of host cell fate?
FASEB J, April 1, 2006; 20(6): 606 - 609.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. J. Reddig, D. Xu, and R. L. Juliano
Regulation of p21-activated Kinase-independent Rac1 Signal Transduction by Nischarin
J. Biol. Chem., September 2, 2005; 280(35): 30994 - 31002.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2003 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.